A5070091608- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Acute Myeloid Leukemia Research
- Diet and metabolism studies
- Metabolism and Genetic Disorders
- Glioma Diagnosis and Treatment
- Analytical Chemistry and Chromatography
- Asthma and respiratory diseases
- Mass Spectrometry Techniques and Applications
- Gut microbiota and health
- Mitochondrial Function and Pathology
- Extracellular vesicles in disease
- Sphingolipid Metabolism and Signaling
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Respiratory and Cough-Related Research
- Chemical Synthesis and Analysis
- Advanced MRI Techniques and Applications
- Lysosomal Storage Disorders Research
University of California, Davis
2015-2023
Metabolon (United States)
2021
Joint Center for Structural Genomics
2017
Urine metabolites are used in many clinical and biomedical studies but usually only for a few classic compounds. Metabolomics detects vastly more metabolic signals that may be to precisely define the health status of individuals. However, compounds remain unidentified, hampering biochemical conclusions. Here, we annotate all detected by two untargeted metabolomic assays, hydrophilic interaction chromatography (HILIC)-Q Exactive HF mass spectrometry charged surface hybrid (CSH)-Q...
Abstract The mammalian brain relies on neurochemistry to fulfill its functions. Yet, the complexity of metabolome and changes during diseases or aging remain poorly understood. Here, we generate a atlas wildtype mouse from 10 anatomical regions spanning adolescence old age. We combine data three assays structurally annotate 1,547 metabolites. Almost all metabolites significantly differ between age groups, but not by sex. A shift in sphingolipid patterns related myelin remodeling is...
Abstract Mitochondrial trifunctional protein deficiency, due to mutations in hydratase subunit A (HADHA), results sudden infant death syndrome with no cure. To reveal the disease etiology, we generated stem cell-derived cardiomyocytes from HADHA-deficient hiPSCs and accelerated their maturation via an engineered microRNA cocktail that upregulated epigenetic regulator, HOPX . Here report, matured HADHA mutant treated endogenous mixture of fatty acids manifest phenotype: defective calcium...
Mesenchymal stem cell (MSC) based therapies are currently being evaluated as a putative therapeutic in numerous human clinical trials. Recent reports have established that exosomes mediate much of the properties MSCs. Exosomes nanovesicles which intercellular communication, transmitting signals between cells regulate diverse range biological processes. MSC-derived packaged with types proteins and RNAs, however, their metabolomic lipidomic profiles to date not been well characterized. We...
Abstract Nonalcoholic fatty liver disease (NAFLD) in non-obese patients remains a clinical condition with unclear etiology and pathogenesis. Using metabolomics approach mouse model that recapitulates almost all the characteristic features of NAFLD, we aimed to advance mechanistic understanding this disorder. Mice fed high fat, cholesterol, cholate (HFHCC) diet for three weeks consistently developed hepatic pathology similar NAFLD nonalcoholic steatohepatitis (NASH) without changes body...
Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects are not well characterized. Since associated diseases as chronic bronchitis asthma, we designed a series of experiments to measure changes in metabolism mice fed obesogenic diets. Mice were either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), very fat (60%kcal fat/7% sucrose) for 150 days. We performed untargeted metabolomics GC-TOFMS...
Mouse knockouts facilitate the study ofgene functions. Often, multiple abnormal phenotypes are induced when a gene is inactivated. The International Phenotyping Consortium (IMPC) has generated thousands of mouse and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples 30 unique corresponding wildtype mice IMPC. To acquire comprehensive data, we used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar lipophilic compounds...
Abstract Alzheimer’s disease (AD) is a major public health priority with large socioeconomic burden and complex etiology. The Alzheimer Disease Metabolomics Consortium (ADMC) the Neuroimaging Initiative (ADNI) aim to gain new biological insights in We report here an untargeted lipidomics of serum specimens 806 subjects within ADNI1 cohort (188 AD, 392 mild cognitive impairment 226 cognitively normal subjects) along 83 quality control samples. Lipids were detected measured using...
Abstract Integration of the unique advantages fields drug discovery and delivery is invaluable for advancement development. Here we propose a self-delivering o ne-component n ew-chemical-entity anomedicine (ONN) strategy to improve cancer therapy through incorporation self-assembly principle into design. A lysosomotropic detergent (MSDH) an autophagy inhibitor (Lys05) are hybridised develop bisaminoquinoline derivatives that can intrinsically form nanoassemblies. The selected BAQ12 BAQ13...
BACKGROUND. Dysregulation of l-arginine metabolism has been proposed to occur in patients with severe asthma. The effects supplementation on metabolite profiles these are unknown. We hypothesized that individuals asthma low fractional exhaled nitric oxide (FeNO) would have fewer exacerbations the addition their standard medications compared placebo and demonstrate greatest changes profiles.
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number high-profile papers the field cancer biology. papers, which were published between 2010 and 2012, on basis citations Altmetric scores (<xref ref-type="bibr" rid="bib3">Errington et al., 2014</xref>). This Registered Report describes proposed replication plan key “The common feature...
Abstract Obesity is considered as a risk factor for COVID‐19 with insulin resistance and increased production of inflammatory cytokines likely mechanisms. Glucagon‐like peptide‐1 (GLP‐1) agonists inhaled nitric oxide are proposed therapeutic approaches to treat because their broad anti‐inflammatory effects. One approach that might augment GLP‐1 levels would be dietary supplementation L‐arginine. Beyond cytokines, multiple studies have started investigate the relationship between new‐onset...
In 2016, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (<xref ref-type="bibr" rid="bib14">Fiehn et al., 2016</xref>), that described how intended to replicate selected experiments from paper "The common feature leukemia-associated IDH1 and IDH2 mutations is neomorphic enzyme activity converting alpha-ketoglutarate 2-hydroxyglutarate" (Ward 2010). Here, report results those experiments. We found cells expressing R172K mutant did not...
Declines in mitochondrial mass are thought to be a hallmark of mammalian aging, and ketogenic diet (KD) may prevent the age-related decreases content. The objective this study was investigate impact KD on markers mass. Mice were fed an isocaloric control (CD) or from 12 months age. Tissues collected after 1 month 14 intervention, panel commonly used (mitochondrial enzyme activities levels, nuclear DNA ratio, cardiolipin content) measured. Our results showed that stimulated marker enzymes...
Ischemic stroke represents a major, worldwide health burden with increasing incidence. Patients affected by ischemic strokes currently have few clinically approved treatment options available. Most treatments for narrow therapeutic windows, severely limiting the number of patients able to be treated. Mesenchymal stem cells represent promising novel stroke. Numerous studies demonstrated that mesenchymal functionally improve outcomes in rodent models Recent also shown exosomes secreted mediate...
Abstract Mouse knockouts allow studying gene functions. Often, multiple phenotypes are impacted when a is inactivated. The International Phenotyping Consortium (IPMC) has generated thousands of mouse and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples 30 corresponding wildtype mice IMPC. To acquire comprehensive data, we used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar lipophilic compounds in an untargeted...