A5085294805- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Metabolomics and Mass Spectrometry Studies
- Cholesterol and Lipid Metabolism
- Inflammatory mediators and NSAID effects
- Liver Disease Diagnosis and Treatment
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Bone Tissue Engineering Materials
- Circular RNAs in diseases
South China University of Technology
2020-2025
Guangzhou Medical University
2023
Guangzhou First People's Hospital
2023
Sun Yat-sen University
2023
Sun Yat-sen University Cancer Center
2023
Jinan University
2023
Red Cross Hospital
2023
State Key Laboratory of Oncology in South China
2023
Southwest Medical University
2023
University of California Davis Medical Center
2007-2017
Abstract Human embryonic stem cells (hESC) may provide a cell source for functional hepatocytes. The aim of this study is to establish viable human hepatocyte-like line from hESC that can be used cell-based therapies. differentiated were enriched by transducing with lentivirus vector containing the green fluorescent protein (GFP) gene driven α1-antitrypsin promoter; GFP expressed in committed hepatocyte progenitors and GFP+ purified laser microdissection pressure catapulting. In addition,...
Abstract Human embryonic stem cells (hESCs) may provide a cell source for functional hepatocytes clinical applications and drug development. Initially, the hESC population was enriched to be more than 85% definitive endoderm (DE) as assessed by expression of CXCR4, SOX17, FOXA2. We then successfully converted DE into hepatic progenitors with 93% being positive α-feto protein within 9 days. The percentage albumin gradually increased 90% at days 20-22 after differentiation. Moreover, our...
Ferredoxin reductase (FDXR), a target of p53, modulates p53-dependent apoptosis and is necessary for steroidogenesis biogenesis iron-sulfur clusters. To determine the biological function FDXR, we generated Fdxr-deficient mouse model found that loss Fdxr led to embryonic lethality potentially due iron overload in developing embryos. Interestingly, mice heterozygous had short life span were prone spontaneous tumors liver abnormalities, including steatosis, hepatitis, hepatocellular carcinoma....
The ability to efficiently generate integration-free induced pluripotent stem cells (iPSCs) from the most readily available source—peripheral blood—has potential expedite advances of iPSC-based therapies. We have successfully generated iPSCs cord blood (CB) CD34+ with improved oriP/EBNA1-based episomal vectors (EV) using a strong spleen focus forming virus (SFFV) long terminal repeat (LTR) promoter. Here we show that Yamanaka factors (OCT4, SOX2, MYC, and KLF4)-expressing EV can also...
Abstract Human induced pluripotent stem cells (hiPSCs) hold great potential for use in regenerative medicine, novel drug development, and disease progression/developmental studies. Here, we report highly efficient differentiation of hiPSCs toward a relatively homogeneous population functional hepatocytes. hiPSC-derived hepatocytes (hiHs) not only showed high expression hepatocyte-specific proteins liver-specific functions, but they also developed biotransformation system including phase I II...
Exosomes secreted from stem cells exerted salutary effects on the fibrotic liver. Herein, roles of exosomes derived human embryonic cell (hESC) in anti-fibrosis were extensively investigated. Compared with two-dimensional (2D) culture, clinical and biological relevance three-dimensional (3D) spheroids greater because their higher regeneration potential since they behave more like vivo. In our study, 3D monolayer hESCs collected compared therapeutic for liver vitro vitro, PKH26...
Abstract Integration of the unique advantages fields drug discovery and delivery is invaluable for advancement development. Here we propose a self-delivering o ne-component n ew-chemical-entity anomedicine (ONN) strategy to improve cancer therapy through incorporation self-assembly principle into design. A lysosomotropic detergent (MSDH) an autophagy inhibitor (Lys05) are hybridised develop bisaminoquinoline derivatives that can intrinsically form nanoassemblies. The selected BAQ12 BAQ13...
Integrin β1 (ITGB1), which acts as an extracellular matrix (ECM) receptor, has gained increasing attention a therapeutic target for the treatment of hepatocellular carcinoma (HCC). However, underpinning mechanism how ITGB1 drives HCC progression remains elusive. In this study, we first found that expression was significantly higher in tissues than normal controls by bioinformatics analysis. Furthermore, analysis revealed paxillin (PXN) and 14-3-3 protein zeta (YWHAZ) are molecules...
Mesenchymal stem cell (MSC) therapies have been brought forward as a promising treatment modality for cutaneous wound healing. However, current approaches delivery many drawbacks, such lack of targetability and loss, leading to poor efficacy therapy. To overcome these problems, in the present study, an situ electrospinning system is developed attractive approach delivery. MSCs high viability over 90% even with applied voltage 15 kV post-cell process. In addition, does not show any negative...
Lipid metabolism plays a critical role in cancer metastasis. However, the mechanisms through which metastatic genes regulate lipid remain unclear. Here, we describe new oncogenic–metabolic feedback loop between epithelial–mesenchymal transition transcription factor ZEB2 and key enzyme ACSL4 (long-chain acyl-CoA synthetase 4), resulting enhanced cellular storage fatty acid oxidation (FAO) to drive breast Functionally, depletion of or significantly reduced droplets (LDs) abundance cell...
ABSTRACT To date, generating viable and functional hepatocytes in large scale remains challenge. By employing 3D suspension condition with the support of low concentration Matrigel, a novel culture system was developed to generate expandable hepatoblast organoids (HB‐orgs) mature polarised hepatocyte (P‐hep‐orgs) from human embryonic stem cells (hESCs) both dishes bioreactors. scRNA‐seq assays were used characterise HB‐orgs P‐hep‐orgs. hESC‐derived could proliferate at least for 15 passages,...
Matrix stiffness is a key factor in breast cancer progression, but its impact on cell function and response to treatment not fully understood. Here, we developed stiffness-tunable hydrogel-based three-dimensional system that recapitulates the extracellular matrix physiological properties of human vitro. Adjusting ratio GelMA PEGDA hydrogel formulation enabled fine-tuning across range 7 52 kPa. Utilizing this (3D) platform for culture has precise functional evaluations. Variations resulted...
The emerging fields of tissue engineering and biomaterials have begun to provide potential treatment options for liver failure. goal the present study is investigate ability a poly L-lactic acid (PLLA) nanofiber scaffold support enhance hepatic differentiation human bone marrow-derived mesenchymal stem cells (hMSCs). A composed collagen was fabricated by electrospinning technique. After characterizing isolated hMSCs, they were seeded onto PLLA scaffolds induced differentiate into hepatocyte...
Background SK Hep-1 cells (SK cells) derived from a patient with liver adenocarcinoma have been considered human hepatoma cell line mesenchymal origin characteristics, however, do not express genes and exhibit function, thus, we hypothesized whether might contribute to primary cancers. Here, characterized its tumourigenicity. Methods Principal Findings We found that classical stem (MSC) markers were presented on cells, but endothelial marker CD31, hematopoietic CD34 CD45 negative. are...
Abstract Background Hematopoietic stem cells are able to self-renew and differentiate into all blood cell lineages. transplantation is a mainstay of life-saving therapy for hematopoietic malignancies hypoproliferative disorders. In vitro differentiation human pluripotent (hPSCs) promising approach modeling development replacement therapies. Although using hPSCs derive progenitor has achieved some successes in the past, from produce which can provide robust long-term multilineage engraftment...
Various studies have shown that lysine acetyltransferase 2A (KAT2A), E2F transcription factor 1 (E2F1), and ubiquitin conjugating enzyme E2 C (UBE2C) genes regulated the proliferation migration of tumor cells through regulating cell cycle. However, there is a lack in-depth systematic research on their mechanisms action. This study analyzed The Cancer Genome Atlas (TCGA) to screen potential candidate regulation network KAT2A E2F1 complex in pan-cancer. Quantitative real-time PCR (qRT-PCR)...
Highlights•A strategy for dynamic control of biomimetic mineralization on various interfaces•Dual role polyphenols as both capping agents and etching agents•Mineralized MPFs with rich bioactive ingredients are obtained•Gelatin-chitosan hydrogel loaded Zn-MPFs@CS exhibits wound healing effectSummaryMineralization is a natural process that constructs intricate structures at diverse interfaces, but poor controllability. Herein, we present an alternative nano-scale processes the subsequent...
Lipid metabolism plays a critical role in cancer metastasis. However, the mechanisms through which metastatic genes regulate lipid remain unclear. Here, we describe new oncogenic-metabolic feedback loop between epithelial-mesenchymal transition transcription factor ZEB2 and key enzyme ACSL4 (long-chain acyl-CoA synthetase 4), resulting enhanced cellular storage fatty acid oxidation (FAO) to drive breast Functionally, depletion of or significantly reduced droplets (LDs) abundance cell...
Background Alcohol insult triggers complex events in the liver, promoting fibrogenic/inflammatory signals and more advanced cases, aberrant matrix deposition. It is well accepted that regenerative capacity of adult liver impaired during alcohol injury. The progenitor/stem cells have been shown to play an important role regeneration -in response various chronic injuries; however, effects on stem cell differentiation are not understood. Methods We employed hepatic progenitor derived from hESCs...
A large number of cancer stem cells (CSCs) were identified and characterized; however, the origins formation CSCs remain elusive. In this study, we examined origination newly CD34(+) liver CSC (LCSC). We found that LCSC coexpressed cell myelomonocytic markers, showing a mixed phenotype, combination hepatobiliary stem/progenitor (HSPCs) cells. Moreover, human xenografts produced by LCSCs parental cells, which was isolated from, also demonstrating phenotypes. The secreted albumin their...
Objectives Normal commitment of the endoderm third pharyngeal pouch (3PP) is essential for development and differentiation thymus. The aim this study was to investigate role transcription factor HOXA3 in 3PP (3PPE) from human embryonic stem cells (hESCs). Methods 3PPE differentiated hESC-derived definitive (DE) by mimicking developmental queues with Activin A, WNT3A, retinoic acid BMP4. function assessed further differentiating into functional thymic epithelial (TECs). effect inhibition on...
CD34(+) stem cells play an important role during liver development and regeneration. Thus, we hypothesized that some human carcinomas (HLCs) might be derived from transformed cells. Here, determined a population of isolated PLC/PRF/5 hepatoma (PLC) appears to function as cancer (LCSCs) by forming HLCs in immunodeficient mice with few 100 Moreover, the PLC subpopulation had advantage over CD34(-) PLCs at initiating tumors. Three types were generated PLC: hepatocellular (HCCs);...