A5014235743- Metabolomics and Mass Spectrometry Studies
- Pancreatic function and diabetes
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Analytical Chemistry and Chromatography
- Diabetes and associated disorders
- Plant biochemistry and biosynthesis
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Metabolism, Diabetes, and Cancer
- Mass Spectrometry Techniques and Applications
- Bioinformatics and Genomic Networks
- Biochemical and biochemical processes
- Diet, Metabolism, and Disease
- 3D Printing in Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Coenzyme Q10 studies and effects
- Alzheimer's disease research and treatments
- Extracellular vesicles in disease
- Nutritional Studies and Diet
- Fatty Acid Research and Health
- Alcohol Consumption and Health Effects
- Liver Disease Diagnosis and Treatment
- Plant Stress Responses and Tolerance
- Spectroscopy and Chemometric Analyses
University of Wisconsin–Madison
2019-2024
William S. Middleton Memorial Veterans Hospital
2023
University of California, Davis
2017-2019
Urine metabolites are used in many clinical and biomedical studies but usually only for a few classic compounds. Metabolomics detects vastly more metabolic signals that may be to precisely define the health status of individuals. However, compounds remain unidentified, hampering biochemical conclusions. Here, we annotate all detected by two untargeted metabolomic assays, hydrophilic interaction chromatography (HILIC)-Q Exactive HF mass spectrometry charged surface hybrid (CSH)-Q...
Untargeted metabolomics by liquid chromatography–mass spectrometry generates data-rich chromatograms in the form of m/z-retention time features. Managing such datasets is a bottleneck. Many popular data processing tools, including XCMS-online and MZmine2, yield numerous false-positive peak detections. Flagging removing false peaks manually time-consuming task prone to human error. We present web application, Mass Spectral Feature List Optimizer (MS-FLO), improve quality feature lists after...
Excess copper may disturb plant photosynthesis and induce leaf senescence. The underlying toxicity mechanism is not well understood. Here, 3-week-old cucumber plants were foliar exposed to different concentrations (10, 100, 500 mg/L) for a final dose of 0.21, 2.1, 10 mg/plant, using CuSO4 as the Cu ion source 7 days, three times per day. Metabolomics quantified 149 primary 79 secondary metabolites. A number intermediates tricarboxylic acid (TCA) cycle significantly down-regulated 1.4–2.4...
Mesenchymal stem cell (MSC) based therapies are currently being evaluated as a putative therapeutic in numerous human clinical trials. Recent reports have established that exosomes mediate much of the properties MSCs. Exosomes nanovesicles which intercellular communication, transmitting signals between cells regulate diverse range biological processes. MSC-derived packaged with types proteins and RNAs, however, their metabolomic lipidomic profiles to date not been well characterized. We...
Mouse knockouts facilitate the study ofgene functions. Often, multiple abnormal phenotypes are induced when a gene is inactivated. The International Phenotyping Consortium (IMPC) has generated thousands of mouse and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples 30 unique corresponding wildtype mice IMPC. To acquire comprehensive data, we used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar lipophilic compounds...
Abstract Alzheimer’s disease (AD) is a major public health priority with large socioeconomic burden and complex etiology. The Alzheimer Disease Metabolomics Consortium (ADMC) the Neuroimaging Initiative (ADNI) aim to gain new biological insights in We report here an untargeted lipidomics of serum specimens 806 subjects within ADNI1 cohort (188 AD, 392 mild cognitive impairment 226 cognitively normal subjects) along 83 quality control samples. Lipids were detected measured using...
Alcohol‐related liver disease is a major public health burden, and the gut microbiota an important contributor to pathogenesis. The aim of present study characterize functional alterations test their performance for short‐term mortality prediction in patients with alcoholic hepatitis. We integrated shotgun metagenomics untargeted metabolomics investigate host co‐metabolism multicenter cohort Profound changes were found microbial composition, metagenome, serum, fecal metabolomes hepatitis...
Metabolomics—the endpoint of the omics cascade—is increasingly recognized as a preferred method for understanding ultimate responses biological systems to stress. Flow injection electrospray (FIE) mass spectrometry (MS) has advantages untargeted metabolic fingerprinting due its simplicity and capability high-throughput screening but requires high-resolution spectrometer resolve metabolite features. In this study, we developed validated highly reproducible metabolomics platform integrating...
Elevated islet production of prostaglandin E2 (PGE2), an arachidonic acid metabolite, and expression receptor subtype EP3 (EP3) are well-known contributors to the β-cell dysfunction type 2 diabetes (T2D). Yet, many same pathophysiological conditions exist in obesity, little is known about how PGE2 signaling pathway influences nondiabetic function. In this work, plasma metabolite levels were quantified a cohort T2D human subjects identify their relationship with glycemic control, systemic...
Human pluripotent stem-cell-derived cardiomyocytes (hPSC-CMs) show immense promise for patient-specific disease modeling, cardiotoxicity screening, and regenerative therapy development. However, thus far, hPSC-CMs in culture have not recapitulated the structural or functional properties of adult CMs vivo. To gain global insight into hPSC-CM biology, we established a multiomics method analyzing metabolome proteome from same cell culture, creating multidimensional profiles hPSC-CMs....
Type 2 diabetes (T2D) is a rising pandemic worldwide. Diet and lifestyle changes are typically the first intervention for T2D. When this fails, biguanide metformin most common pharmaceutical therapy. Yet its full mechanisms of action remain unknown. In work, we applied an ultrahigh resolution, mass spectrometry-based platform untargeted plasma metabolomics to human samples from case-control observational study nondiabetic well-controlled T2D subjects, latter treated conservatively with or...
The heart contracts incessantly and requires a constant supply of energy, utilizing numerous metabolic substrates, such as fatty acids, carbohydrates, lipids, amino to its high energy demands. Therefore, comprehensive analysis various metabolites is urgently needed for understanding cardiac metabolism; however, complete metabolome analyses remain challenging due the broad range metabolite polarities, which makes extraction detection difficult. Herein, we implemented parallel extractions...
Scope High‐sodium and low‐potassium (HNaLK) content in Western diets increases the risk of hypertension cardiovascular disease (CVD). It is investigated if dietary minerals interact with gut bacteria to modulate circulating levels biogenic amines, which are implicated various pathologies, including CVD. Methods results Using a metabolomic approach target effects depletion HNaLK intake on amines rats examined. Forty‐five metabolites whose plasma significantly altered by ( p < 0.05) found,...
Abstract Mouse knockouts allow studying gene functions. Often, multiple phenotypes are impacted when a is inactivated. The International Phenotyping Consortium (IPMC) has generated thousands of mouse and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples 30 corresponding wildtype mice IMPC. To acquire comprehensive data, we used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar lipophilic compounds in an untargeted...
Multiomic studies are increasingly performed to gain a deeper understanding of molecular processes occurring in biological system, such as the complex microbial communities (i.e., microbiota) that reside distal gut. While combination metabolomics and proteomics is more commonly used, multiomics including peptidomcis characterization less frequently undertaken. Here, we investigated three different extraction methods, chosen for their previous use extracting metabolites, peptides, proteins,...
Abstract The heart contracts incessantly and requires a constant supply of energy, utilizing numerous metabolic substrates such as fatty acids, carbohydrates, lipids, amino acids to its high energy demands. Therefore, comprehensive analysis various metabolites is urgently needed for understanding cardiac metabolism; however, complete metabolome analyses remain challenging due the broad range metabolite polarities which makes extraction detection difficult. Herein, we implemented parallel...
Alzheimer's disease (AD) associated dementia is a global health problem for the aging population. It will be major and economic burden on society by 2050. There are few treatment options available AD but they have poor efficacy. An active research ongoing to identify biological entities which can implicated in etiology. A range of studies show that altered lipid metabolism with AD. We analyzed serum lipidome using liquid chromatography mass spectrometry cross-sectional study 200 cognitively...
Abstract Elevated islet production of prostaglandin E 2 (PGE ), an arachidonic acid metabolite, and expression Prostaglandin Receptor subtype EP3 (EP3) are well-known contributors to the β-cell dysfunction type diabetes (T2D). Yet, many same pathophysiological conditions exist in obesity, little is known about how PGE signaling pathway influences non-diabetic beta-cell function. In this work, plasma metabolite levels were quantified a cohort T2D human subjects identify their relationship...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) show immense promise for patient-specific disease modeling, cardiotoxicity screening, and regenerative therapy development. However, hPSC-CMs in culture have not recapitulated the structural or functional properties of adult CMs vivo thus far. To gain global insight into hPSC-CM biology, we established a multi-omics method analyzing metabolome proteome from same cell culture, creating multi-dimensional profiles hPSC-CMs....