A5053594078- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Metabolomics and Mass Spectrometry Studies
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- Neurotransmitter Receptor Influence on Behavior
- Cardiac Structural Anomalies and Repair
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
University of Wisconsin–Madison
2021-2025
Brown University
2020
University of Rhode Island
2019
Global bottom-up mass spectrometry (MS)-based proteomics is widely used for protein identification and quantification to achieve a comprehensive understanding of the composition, structure, function proteome. However, traditional sample preparation methods are time-consuming, typically including overnight tryptic digestion, extensive cleanup remove MS-incompatible surfactants, offline fractionation reduce proteome complexity prior online liquid chromatography-tandem (LC-MS/MS) analysis....
Ischemic cardiomyopathy (ICM) is a prominent form of heart failure, but the molecular mechanisms underlying ICM remain relatively understudied due to marked phenotypic heterogeneity. Alterations in post-translational modifications (PTMs) and isoform switches sarcomeric proteins play important roles cardiac pathophysiology. Thus, it essential define proteoform landscape better understand ICM. Herein, we have implemented top-down liquid chromatography (LC)–mass spectrometry (MS)-based...
Dynamic tyrosine phosphorylation is fundamental to a myriad of cellular processes. However, the inherently low abundance in proteome and inefficient enrichment phosphotyrosine(pTyr)-containing peptides has led poor pTyr peptide identification quantitation, critically hindering researchers' ability elucidate signaling pathways regulated by systems where material limited. The most popular approaches wide-scale characterization phosphoproteome use with pan-specific, anti-pTyr antibodies from...
Protein biomarkers in human serum provide critical insights into various physiological conditions and diseases, enabling early diagnosis, prognosis, personalized treatment. However, detecting low-abundance protein is challenging due to the presence of highly abundant proteins that make up ∼99% plasma proteome. Here, we report use situ metal–organic framework (MOF) growth effectively deplete for integrated proteomic analysis. Through biomolecule-mediated nucleation a zeolitic imidazolate...
Exosomes are small extracellular vesicles (EVs) secreted by all cells and found in biological fluids, which can serve as minimally invasive liquid biopsies with extremely high therapeutic diagnostic potential. Mass spectrometry (MS)-based proteomics is a powerful technique to profile quantify the protein content exosomes, but current methods require laborious time-consuming multistep sample preparation that significantly limit throughput. Herein, we report one-pot exosome method enabled...
While Lck has been widely recognized to play a pivotal role in the initiation of T cell receptor (TCR) signaling pathway, an understanding precise regulation cells upon TCR activation remains elusive. Investigation protein–protein interaction (PPI) using proximity labeling techniques such as TurboID potential provide valuable molecular insights into regulatory networks. By expressing Lck-TurboID Jurkat cells, we have uncovered dynamic, short-range protein network 30 min stimulation. In this...
Human pluripotent stem-cell-derived cardiomyocytes (hPSC-CMs) show immense promise for patient-specific disease modeling, cardiotoxicity screening, and regenerative therapy development. However, thus far, hPSC-CMs in culture have not recapitulated the structural or functional properties of adult CMs vivo. To gain global insight into hPSC-CM biology, we established a multiomics method analyzing metabolome proteome from same cell culture, creating multidimensional profiles hPSC-CMs....
Dilated cardiomyopathy (DCM) is a major risk factor for developing heart failure and often associated with an increased life-threatening arrhythmia. Although numerous causal genes DCM have been identified, RNA binding motif protein 20 (Rbm20) remains one of the few splicing factors that, when mutated or genetically ablated, leads to development DCM. In this study we sought identify changes in cardiac proteome Rbm20 knockout (KO) rat hearts using global quantitative proteomics gain insight...
Three-dimensional engineered cardiac tissue (ECT) using purified human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has emerged as an appealing model system for the study of biology and disease. A recent reported widely used metabolic (lactate) purification monolayer hiPSC-CM cultures results in ischemic cardiomyopathy-like phenotype compared with magnetic antibody-based cell sorting (MACS) purification, complicating interpretation studies lactate-purified hiPSC-CMs....
The metabolic switch from glycolysis to fatty acid oxidation in postnatal cardiomyocytes contributes the loss of cardiac regenerative potential mammalian heart. However, mechanisms that regulate this remain unclear. protein kinase complex mechanistic target rapamycin 1 (mTORC1) is a central signaling hub regulates cellular metabolism and synthesis, yet its role during heart regeneration maturation undefined. Here, we use immunoblotting, treatment, myocardial infarction, global proteomics...
ABSTRACT Adult mammalian cardiomyocytes have limited proliferative potential, and after myocardial infarction (MI), injured cardiac tissue is replaced with fibrotic scar rather than functioning myocardium. In contrast, the neonatal mouse heart possesses a regenerative capacity governed by cardiomyocyte proliferation; however, metabolic switch from glycolysis to fatty acid oxidation during postnatal development results in loss of this capacity. Interestingly, sarcomere isoform also takes...
Abstract Global bottom-up mass spectrometry (MS)-based proteomics is widely used for protein identification and quantification to achieve a comprehensive understanding of the composition, structure, function proteome. However, traditional sample preparation methods are time-consuming, typically including overnight tryptic digestion, extensive clean-up remove MS-incompatible surfactants, offline fractionation reduce proteome complexity prior online liquid chromatography-tandem (LC-MS/MS)...
Myosin functions as the "molecular motor" of sarcomere and generates contractile force necessary for cardiac muscle contraction. light chains 1 2 (MLC-1 -2) play important functional roles in regulating structure hexameric myosin molecule. Each these has an "atrial" "ventricular" isoform, so called because they are believed to exhibit chamber-restricted expression heart. However, recently chamber-specific MLC isoforms human heart been questioned. Herein, we analyzed MLC-1 -2 atrial...
Three-dimensional engineered cardiac tissue (ECT) using purified human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has emerged as an appealing model system for the study of biology and disease. A recent reported widely-used metabolic (lactate) purification monolayer hiPSC-CM cultures results in ischemic cardiomyopathy-like phenotype compared to magnetic antibody-based cell sorting (MACS) purification, complicating interpretation studies lactate-purified hiPSC-CMs....
Abstract Exosomes are small extracellular vesicles (EVs) secreted by all cells and found in biological fluids, which can serve as minimally invasive liquid biopsies with high therapeutic diagnostic potential. Mass spectrometry (MS)-based proteomics is a powerful technique to profile quantify the protein content of exosomes but current methods require laborious time-consuming multi-step sample preparation that significantly limit throughput. Herein, we report one-pot exosome method enabled...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) show immense promise for patient-specific disease modeling, cardiotoxicity screening, and regenerative therapy development. However, hPSC-CMs in culture have not recapitulated the structural or functional properties of adult CMs vivo thus far. To gain global insight into hPSC-CM biology, we established a multi-omics method analyzing metabolome proteome from same cell culture, creating multi-dimensional profiles hPSC-CMs....