A5010529715- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Immunodeficiency and Autoimmune Disorders
- Ginger and Zingiberaceae research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Gut microbiota and health
- Folate and B Vitamins Research
- Epigenetics and DNA Methylation
- Barrier Structure and Function Studies
- DNA Repair Mechanisms
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
Kumamoto University
2013-2024
Osaka International University
2016-2023
Osaka University
2016-2023
Toxicologie, Pharmacologie et Signalisation Cellulaire
2022
Significance Idiopathic inflammatory bowel disease (IBD) is associated with the gut microbiota and immune system of host; however, precise pathogenesis IBD poorly defined. We show that specific deletion endoribonuclease Regnase-1 in intestinal epithelial cells relieves symptoms experimental colitis during acute inflammation. deficiency potentiates mTOR signaling purine metabolism colon epithelium. These data provide insight into role IBD.
Accumulating evidence suggests that high levels of Fusobacterium nucleatum in colorectal tumor tissues can be associated with poor prognosis patients cancer (CRC); however, data regarding distinct prognostic subgroups F. nucleatum–positive CRC remain limited. Herein, we demonstrate high-iron status was a worse nucleatum. Patients presenting elevated serum transferrin saturation exhibited preferential iron deposition macrophages the microenvironment. In addition, induced CCL8 expression via...
Accumulating evidence suggests an association between iron metabolism and lung cancer progression. In biological systems, is present in either reduced (Fe2+ ; ferrous) or oxidized (Fe3+ ferric) states. However, ferrous ferric exhibit distinct chemical properties, the role of cell growth has not been clearly distinguished. this study, we manipulated balance cellular status by inducing gene mutations involving FBXL5-IRP2 axis, a ubiquitin-dependent regulatory system for homeostasis, determined...
Adjuvants are essential substances for vaccines and immunotherapies that enhance antigen-specific immune responses. Single-stranded oligodeoxynucleotides containing an unmethylated CpG motif (CpG ODNs) agonistic ligands toll-like receptor 9 initiate innate response. They represent promising adjuvants antiviral antitumor immunotherapies; however, ODNs have some limitations, such as poor nuclease resistance low cell membrane permeability. Therefore, effective formulation is needed to improve...
Cancer cells adeptly manipulate the tumor microenvironment (TME) to evade host antitumor immunity. However, role of cancer cell-intrinsic signaling in shaping immunosuppressive TME remains unclear. Here, we found that Hippo pathway orchestrates by influencing composition cancer-associated fibroblasts (CAFs). In a 4T1 mouse breast model, kinases, large suppressor 1 and 2 (LATS1/2), promoted formation neural cell adhesion molecule (NCAM1)
Abstract APOBEC3s (A3s) are single-stranded DNA cytosine deaminases that provide innate immune defences against retroviruses and mobile elements. A3s specific to eutherian mammals because no direct homologs exist at the syntenic genomic locus in metatherian (marsupial) or prototherian (monotreme) mammals. However, these species have likely evolutionary precursors, antibody gene deaminase AID RNA/DNA editing enzyme APOBEC1 (A1). Here, we used cell culture-based assays determine whether...
RNA export factor germinal center-associated nuclear protein (GANP) interacts with activation-induced cytidine deaminase (AID) and shepherds it from the cytoplasm to nucleus toward IgV region loci in B cells. In this study, we demonstrate a role for GANP repair of AID-initiated DNA damage chicken DT40 cells generate diversity by gene conversion somatic hypermutation. plays positive diversification nonhomologous end joining-proficient state. DNA-PKcs physically GANP, interaction is...
Abstract The ssDNA-dependent deoxycytidine deaminase apolipoprotein B mRNA–editing, enzyme-catalytic, polypeptide-like 3G (A3G) is a potent restrictive factor against HIV-1 virus lacking viral-encoded infectivity (Vif) in CD4+ T cells. A3G antiretroviral activity requires its encapsulation into virions. In this study, we show that germinal center–associated nuclear protein (GANP) induced activated cells and physically interacts with A3G. Overexpression of GANP augments the encapsidation...
Immunoglobulin affinity maturation depends on somatic hypermutation (SHM) in immunoglobulin variable (IgV) regions initiated by activation-induced cytidine deaminase (AID). AID induces transition mutations C→U deamination both strands, causing C:G→T:A. Error-prone repairs of U base excision and mismatch (MMRs) create transversion at C/G A/T sites. In Neuberger's model, it remained to be clarified how transition/transversion repair is regulated. We investigate the role AID-interacting GANP...
<title>Abstract</title> Tumor cells adeptly manipulate the tumor microenvironment (TME) to evade host antitumor immunity. However, role of cell-intrinsic signaling in shaping immunosuppressive TME remains unclear. Here, we found that Hippo pathway orchestrated by influencing composition cancer-associated fibroblasts (CAFs). In a 4T1 mouse breast cancer model, kinases, large suppressor 1 and 2 (LATS1/2), promoted formation neural cell adhesion molecule (NCAM1)+alpha-smooth muscle actin...
<title>Abstract</title> Cancer immunotherapy, particularly using immune checkpoint inhibitors, has revolutionized cancer treatment; however, its efficacy remains limited to a subset of patients. Nanoparticles have potential in treatment because they offer advantages such as biocompatibility, greater stability, and precise targeting capabilities. In this study, we investigated the an integrated glyco-nanovaccine (iGN) comprising gold nanoparticles conjugated with synthetic Toll-like receptor...
The RNA-binding protein (RBP) Regnase-1 is an endonuclease that regulates immune responses by modulating target mRNA stability. degrades a group of inflammation-associated mRNAs, which contributes to balanced response and helps prevent autoimmune diseases. also cleaves its own binding stem-loop (SL) RNA structures in 3'UTR. To understand how this autoregulation important for responses, we generated mice with 2-bp genome deletion the SL 3'-untranslated region (3'UTR). Deletion these...
Abstract Epigenetic modification at IgV-region gene is the key base to generate somatic hypermutation (SHM) mediated by activation-induced cytidine deaminase (AID). However, details how AID-targeting rearranged locus have remained unclear. As a player, we demonstrate that histone-acetyl transferase activity of germinal center-associated protein GANP necessary for efficient AID-accession. upregulated in center B-cells upon T-cell dependent antigen responses. forms complex with AID and...