A5000497626- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Fibrosis and Remodeling
- Ion Transport and Channel Regulation
- Chemical Synthesis and Analysis
- Pediatric Hepatobiliary Diseases and Treatments
- Advanced Glycation End Products research
- AI in cancer detection
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Inflammatory mediators and NSAID effects
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- MicroRNA in disease regulation
- Immune cells in cancer
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Biomedical Research and Pathophysiology
Siriraj Hospital
2012-2024
Mahidol University
2012-2024
Milpark Hospital
2024
Bangkok Hospital
2020-2024
Hodges University
2020
Weatherford College
2020
National Nanotechnology Center
2017
Khon Kaen University
2002-2011
University of Wisconsin–Madison
2006
National Science and Technology Development Agency
2002
Abstract Background Fibroblasts play important roles in several cancers. It was hypothesized that cholangiocarcinoma (CCA)-associated fibroblasts (Cfs) differ from non-tumorigenic liver (Lfs) their gene expression profiles resulting the capability to promote cancer. Periostin (PN) is a multi-functional protein and has emerged as promising marker for tumor progression. The role of PN CCA, however, not yet been explored. Results In this study, profile Cfs comparison Lfs performed using...
Cancer-associated fibroblasts and high mobility group box 1 (HMGB1) protein have been suggested to mediate cancer progression chemotherapy resistance. The role of such in HMGB1 production breast is unclear. This study aimed investigate the effects cancer-associated on expression cells its chemotherapeutic response. Breast (BCFs) non-tumor-associated (NTFs) were isolated from human cancers or adjacent normal tissues established as primary cultures vitro. After confirmation activated status...
Abstract Background Carcinoma-associated fibroblasts (CAFs) play a critical role in cancer progression and immune cell modulation. In this study, it was aimed to evaluate the roles of CAFs-derived IL-6 doxorubicin (Dox) resistance PD-L1-mediated chimeric antigenic receptor (CAR)-T breast (BCA). Methods CAF conditioned-media (CM) were collected, level measured by ELISA. CAF-CM treated MDA-MB-231 HCC70 TNBC lines si (IL-6R) knocked down (KD) cells determine effect CAF-derived on Dox flow...
Periostin (PN) is mainly produced from stromal fibroblasts in cholangiocarcinoma (CCA) and shows strong impact cancer promotion. This work aimed to investigate the mechanism that PN uses drive CCA invasion. It was found ITGα5β1 α6β4 showed high expression non-tumorigenic biliary epithelial cells almost all cell lines. had preferential binding via blocking this receptor by either neutralizing antibody or siITGα5 could attenuate PN-induced After PN-ITGα5β1 binding, intracellular pAKT...
High-mobility group box 1 (HMGB1) is increased in breast cancer cells as the result of exposure to secreted substances from cancer-associated fibroblasts and plays a crucial role progression drug resistance. Its effect, however, on expression programmed death ligand (PD-L1) has not been investigated. This study aimed investigate mechanism HMGB1 through receptors for advanced glycation end products (RAGE) cell migration/invasion PD-L1 cells.A 3-dimensional (3-D) migration invasion assay...
The treatment of breast cancer (BC) remains a formidable challenge due to the emergence drug resistance, necessitating exploration innovative strategies. Chimeric antigen receptor (CAR)-T cell therapy, groundbreaking approach in hematologic malignancies, is actively under investigation for its potential application solid tumors, including BC. Trophoblast surface 2 (Trop2) has emerged as promising immunotherapeutic target various cancers and notably overexpressed To enhance therapeutic...
Abstract Breast cancer stands as a formidable global health challenge for women. While neoantigens exhibit efficacy in activating T cells specific to and instigating anti-tumor immune responses, the accuracy of neoantigen prediction remains suboptimal. In this study, we identified from patient-derived breast cells, PC-B-142CA PC-B-148CA utilizing whole-genome RNA sequencing. The pVAC-Seq pipeline was employed, with minor modification incorporating criteria (1) binding affinity mutant (MT)...
Infection by Opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. However, the mechanism which parasite involved in carcinogenesis not clear. In addition to direct damage of bile duct epithelium via contact with O. viverrini, excretory/secretory (ES) product(s) released from parasites may play important roles this process. We therefore investigated responses fibroblast cell line, NIH-3T3, ES using non-contact co-culture technique. culture system, upper chamber had no...
Cholangiocarcinoma (CCA) has an abundance of tumor stroma which plays important role in cancer progression via tumor-promoting signals. This study aims to explore the microRNA (miRNA) profile CCA-associated fibroblasts (CCFs) and roles any identified miRNAs CCA progression.miRNA expression profiles CCFs normal skin were compared by microarray. Identified downregulated their target genes confirmed real-time PCR. Their binding was a luciferase reporter assay. The effects conditioned-media (CM)...
Dendritic cell (DC)-based T-cell activation is an alternative immunotherapy in breast cancer. The anti-programmed death ligand 1 (PD-L1) can enhance function. Nucleolin (NCL) overexpressed triple-negative cancer (TNBC). regulation of PD-L1 expression through autophagy and the anti-PD-L1 peptide to help sensitize T cells for NCL-positive TNBC killing has not been evaluated. Results showed worst clinical outcome patients with high NCL PD-L1. Self-differentiated myeloid-derived...
Paclitaxel (PTX) is a potent anti-cancer drug commonly used for the treatment of advanced breast cancer (BCA) and melanoma. Toll-like receptor 4 (TLR4) promotes production pro-inflammatory cytokines associated with chemoresistance. This study aims to explore effect TLR4 in PTX resistance triple-negative BCA melanoma compound A (CpdA) attenuate this resistance.BCA cell lines were checked response by cytotoxic assay. The TLR4-transient knockdown cells siRNA transfection was evaluated compared...
Abstract Colorectal cancer (CRC) is one of the most fatal cancers with highly invasive properties. The progression CRC determined by driving force periostin (PN) from cancer‐associated fibroblasts (CAFs) in tumour microenvironment. This present work aims to investigate autophagy‐mediated invasion via receptor integrin (ITG) PN. level PN 410 clinical tissues was found increased and an independent poor prognosis marker (HR = 2.578, 95% CI 1.218‐5.457, P ‐value .013) a significant correlation...
The tumor microenvironment composed of a mixture stromal cells and their secretions has marked impact on cancer progression. In particular, soluble factors metabolites contribute to malignancy through the dysregulation autophagy in cells. present study investigated effects ovarian cancer‑associated fibroblasts (OVCAFs) with secretory substances migration conditioned‑medium (CM) OVCAFs isolated from fresh human tissues was analyzed for levels 27 common cytokines/chemokines using cytokine...
Abstract Background Chemoresistance is one of the main problems in treatment cancer. Periostin (PN) a stromal protein which mostly secreted from cancer associated fibroblasts tumor microenvironment and can promote progression including cell survival, metastasis, chemoresistance. The objective this study was to develop an anti-PN peptide bacteriophage library overcome PN effects breast (BCA) cells. Methods A twelve amino acids display used for biopanning against active site. selected clone...
IntroductionThe influence of cancer-associated fibroblasts (CAFs) and high mobility group box 1 (HMGB1) has been recognized in several cancers, although their roles breast cancer are unclear. The present study aimed to determine the levels prognostic significance α-smooth muscle actin-positive (ASMA+) CAFs, plus HMGB1 receptor for advanced glycation end products (RAGE) cells.Materials MethodsA total 127 samples, including 96 malignant 31 benign, were examined ASMA, HMGB1, RAGE by...