Abstract Background Fibroblasts play important roles in several cancers. It was hypothesized that cholangiocarcinoma (CCA)-associated fibroblasts (Cfs) differ from non-tumorigenic liver (Lfs) their gene expression profiles resulting the capability to promote cancer. Periostin (PN) is a multi-functional protein and has emerged as promising marker for tumor progression. The role of PN CCA, however, not yet been explored. Results In this study, profile Cfs comparison Lfs performed using...

Periostin (PN) is mainly produced from stromal fibroblasts in cholangiocarcinoma (CCA) and shows strong impact cancer promotion. This work aimed to investigate the mechanism that PN uses drive CCA invasion. It was found ITGα5β1 α6β4 showed high expression non-tumorigenic biliary epithelial cells almost all cell lines. had preferential binding via blocking this receptor by either neutralizing antibody or siITGα5 could attenuate PN-induced After PN-ITGα5β1 binding, intracellular pAKT...

Caffeic acid phenethyl ester (CAPE), a natural phenolic compound, has demonstrated antibacterial effects. Dental caries etiology is multifactorial, including cariogenic biofilm containing multispecies bacteria. However, the property of CAPE on unclear. The aim this study was to assess effect formation and cariogenicity in Streptococcus mutans, oralis, mitis. S. mutans (ATCC 25175), oralis 35037), mitis 49456T) were employed investigation. Each bacterial strain cultured presence CAPE,...

Type 2 diabetes mellitus is a major risk factor for melioidosis, which caused by Burkholderia pseudomallei. Our previous study has shown that polymorphonuclear neutrophils (PMNs) from diabetic subjects exhibited decreased functions in response to B. Here we investigated the mechanisms regulating cytokine secretion of PMNs patients might contribute patient susceptibility bacterial infections. Purified who had been treated with glibenclamide (an ATP-sensitive potassium channel blocker...

Background: Head and neck squamous cell carcinoma (HNSCC) has a yearly incidence of 600,000 cases worldwide with low survival rate. Ocimum sanctum L. or tenuiflorum (Holy basil; Tulsi in Hindi), is traditional medicine herb that demonstrates numerous effects including anti-oxidant, anti-microbial, anti-tumor effects. The aim this study was to evaluate the anti-invasive effect O. leaf extract on HNSCC lines. Methods: Ethanolic (EEOS) prepared phenolic compounds were identified using...

Background: Propolis, a resinous substance produced by the honeybee, has wide spectrum of potent biological activities. However, anti-cancer activity propolis obtained from Trigona sirindhornae, new species stingless bee, not yet been reported. This study concerned cytotoxicity extracts T. sirindhornae against two head and neck squamous cell carcinoma (HNSCC) lines. Materials Methods: A dichloromethane extract (DMEP) was prepared generating 3 fractions: DMEP-A, DMEP-B, DMEP-C....

Neutrophils play a key role in the control of Burkholderia pseudomallei, pathogen that causes melioidosis. Here, we show survival intracellular B. pseudomallei was significantly increased presence 3-methyladenine or lysosomal cathepsin inhibitors. The LC3-flux pseudomallei-infected neutrophils. Concordant with this result, confocal microscopy analyses using anti-LC3 antibodies revealed pseudomallei-containing phagosomes partially overlapped LC3-positive signal at 3 and 6 h postinfection....

Background: Dental caries commonly occurs during orthodontic treatment because fixed appliances can impede effective oral hygiene practices. This study investigated the effects of on dental biofilm maturity and virulence gene (gtfB, ldh, brpA, spaP, luxS, gbpB) expression. Methods: biofilms expression were determined in 24 patients before after ≥6 months. A three-tone disclosing gel was used to stain assess its by color change—pink (new biofilm), purple (mature light blue (cariogenic...

Mucin 1 (MUC1) is a transmembrane glycoprotein that contributes to the cellular response in hypoxic conditions different carcinomas. We investigated gene expression pattern of MUCs (1, 2, 4, 5AC, 5B, 6, 15, 16, and 19) isogenic primary (HN4 HN30) metastatic (HN12 HN31) head neck squamous cell carcinoma (HNSCC) lines. MUC1 was significantly up-regulated at mRNA protein levels HN12 HN31 cells, whereas, other exhibited diverse patterns between HNSCC Immunohistochemistry demonstrated exclusively...

The aim of this study was to evaluate the effects synthetic peptides on growth and biofilm formation Streptococcus mutans their cytotoxicity a human keratinocyte cell line. Four pomegranate-derived peptides; Pug-1, Pug-2, Pug-3 Pug-4 were synthesized. (ATCC 25175 DMST 41283) used. antibacterial anti-adherence activities evaluated. effect S. glucosyltransferase (Gtf) activity measured. peptides' evaluated HaCaT line using an MTT assay. bactericidal penetration depth Pug-1 into investigated...

Nitric oxide (NO) functions have been studied in many cancer types, but rarely head and neck squamous cell carcinoma (HNSCC). This study aimed to investigate the behavior of HNSCC cells following exposure high NO (HNO).Two pairs isogenic lines (HN18/HN17, HN30/HN31) were used, treated with a donor for 72 h. Cell viability, cycle, apoptosis, invasion, MMP activity determined using MTT, flow cytometry, Matrigel gelatinase zymography assays, respectively.HNO induced HN18 HN31 cycle progression...

Propolis has demonstrated wound healing effects. Propolis' effects vary based on its composition and geographical origin. However, there are few reports the of propolis oral healing. The aim this study was to evaluate antioxidant in vitro gingival n-hexane extract (HEP), ethyl acetate (EEP), aqueous (AEP) fractions ethanol Thai propolis.The crude obtained by maceration with 95% that sequentially fractionated hexane, acetate, distilled water. chemical profiles samples were assessed thin-layer...

Glycolysis is a key energy-providing process and one of the hallmarks cancer. Nitric oxide (NO), free radical molecule, regulates glycolysis in various cancers. NO can alter cell cycle apoptosis head neck squamous carcinoma (HNSCC) cells. However, effect on HNSCC cells remains unresolved. The present study investigated effects proliferation, glucose transporter (GLUT) gene expression glycolytic indicators lines. Two pairs isogenic lines, HN18/HN17 HN30/HN31, were treated with donor,...

Periostin (PN) (also known as osteoblast‑specific factor OSF‑2) is a protein that in humans encoded by the POSTN gene and has been correlated with reduced survival of cholangiocarcinoma (CCA) patients, well‑known effect inducing epithelial‑to‑mesenchymal transition (EMT). The present study investigated PN, through integrin (ITG)α5β1, EMT‑mediated CCA aggressiveness. alterations EMT‑related expression were real‑time PCR, western blot analysis zymogram. effects PN on migration level TWIST‑2...

Propolis is a resinous product accumulated from several plant sources that possess wide range of therapeutic properties, including anti-cancer activities. However, the role honeybee-produced propolis on head and neck squamous carcinoma (HNSCC) not well understood. The aim this study was to investigate effects Apis mellifera apoptosis invasiveness in HNSCC cell lines.Ethyl acetate extract (EAEP) prepared A. beehives using liquid-liquid extraction. High-performance liquid chromatography...

Autophagy is an intracellular degradative process occurring under stressful conditions. Nitric oxide (NO), a free radical, regulates autophagy and apoptosis in several cancers. However, the effect of head neck squamous cell carcinoma (HNSCC) adaptation to high nitric (HNO) on remains unknown. We investigated apoptotic changes HNO-adapted HNSCC lines.

To evaluate the efficacy of positive-pressure nasal irrigation devices in children with acute sinusitis, addition to bacterial colonization device.We performed a randomized, prospective, controlled study 80 aged between 3 and 15 years. Participants were randomly separated into two groups, where one group was treated using squeezable bottle other syringe. All patients instructed use 1.25% buffered hypertonic solution for twice daily 2 weeks, amoxicillin-clavulanic acid. During this period,...

Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine that contributes to the progression of several cancers. MIF overexpression has been reported in head and neck squamous cell carcinoma (HNSCC) patients. However, exact role HNSCC not fully understood. Our aim was evaluate amount secreted proliferation, cycle, apoptosis lines.Genetically matched lines derived from primary (HN18 HN30) metastatic sites (HN17 HN31) same patient were used this study. The levels conditioned...

Abstract Head and neck squamous cell carcinoma (HNSCC) has a yearly incidence of 600,000 cases worldwide with low survival rate. Ocimum sanctum L. or tenuiflorum (Holy basil; Tulsi in Hindi), is traditional medicine herb that demonstrates numerous effects, including anti-oxidant, anti-microbial, anti-tumor effects. The aim this study was to evaluate the anti-invasive effect O. leaf extract on HNSCC lines. Ethanolic (EEOS) prepared phenolic compounds were identified using high-performance...

Ocimum sanctum Linn. is a medicinal herb that has cytotoxic effects by inducing oxidative stress in some carcinomas. This study aimed to examine the impact of O. leaf extract on stress, cell cycle progression, and apoptosis lines head neck squamous carcinoma (HNSCC). Isogenic primary (HN18/HN30) metastatic (HN17/HN31) HNSCC were used. Preparation ethanolic O.sanctum (EEOS) was carried out. exposed varying concentrations (0.1-0.8 mg/ml) EEOS for duration 72 h, MTT assay utilized determine...

Increased dental biofilm commonly occurs during orthodontic treatment. The aim of this study was to evaluate the effect a combined toothbrushing method on cariogenicity in patients with stainless steel (SSL) and elastomeric (EL) ligatures. At baseline (T1), 70 participants were randomized (1:1 ratio) SSL or EL group. Dental maturity evaluated using three-color-disclosing dye. instructed brush their teeth horizontal–Charters–modified Bass technique. reassessed at 4-week follow-up (T2). We...