Head and neck squamous cell carcinoma (HNSCC) is a complex tissue that contains tumor cells the surrounding stroma, which populated by different types of mesenchymal extracellular matrix (ECM).Collectively, they are referred to as microenvironment (TME).Recent studies have shown TME has more profound influence on growth metastasis HNSCC than was previously appreciated.Because carcinoma-associated fibroblasts (CAFs) frequently observed in stroma tumor, this review focuses potential role...

Abstract Three heat‐cured and three autopolmerized arcylic denture bases with different mixing proportions and/or processing methods were investigated for the amount of resdidual monomer content methyl methacrylate (MMA) released into saliva after incubation during first second 24 house processing. A corresponding range concentrations mma was also used to test cell cytotoxicity using a culture human oral fibroblasts. The results showed that residual dependent not only on type polymerization...

Caffeic acid phenethyl ester (CAPE), a natural phenolic compound, has demonstrated antibacterial effects. Dental caries etiology is multifactorial, including cariogenic biofilm containing multispecies bacteria. However, the property of CAPE on unclear. The aim this study was to assess effect formation and cariogenicity in Streptococcus mutans, oralis, mitis. S. mutans (ATCC 25175), oralis 35037), mitis 49456T) were employed investigation. Each bacterial strain cultured presence CAPE,...

Background: Head and neck squamous cell carcinoma (HNSCC) has a yearly incidence of 600,000 cases worldwide with low survival rate. Ocimum sanctum L. or tenuiflorum (Holy basil; Tulsi in Hindi), is traditional medicine herb that demonstrates numerous effects including anti-oxidant, anti-microbial, anti-tumor effects. The aim this study was to evaluate the anti-invasive effect O. leaf extract on HNSCC lines. Methods: Ethanolic (EEOS) prepared phenolic compounds were identified using...

Background/Objectives: Early detection of biological changes is crucial for effective monitoring orthodontic tooth movement (OTM). Research that elucidates the connection between OTM and its proteomic profile remains limited. This study aimed to identify salivary protein biomarkers specific fixed appliances (FOA) Invisalign (IN) treatment modalities. Methods: Unstimulated saliva was collected from 17 patients treated with FOA 6 IN before (T0), at 3 (T1) (T2) months after therapy initiation....

Background: Propolis, a resinous substance produced by the honeybee, has wide spectrum of potent biological activities. However, anti-cancer activity propolis obtained from Trigona sirindhornae, new species stingless bee, not yet been reported. This study concerned cytotoxicity extracts T. sirindhornae against two head and neck squamous cell carcinoma (HNSCC) lines. Materials Methods: A dichloromethane extract (DMEP) was prepared generating 3 fractions: DMEP-A, DMEP-B, DMEP-C....

Clinacanthus nutans (C. nutans) has demonstrated anti-inflammatory activity, however, the active compound generating this activity remains unknown. The aim of study was to identify bioactive in C. responsible for its anti-inflammatory, in-vitro wound healing, and anti-biofilm activities.A pure isolated from chloroform extract (CE) leaves by chromatographic techniques bioassay-guided fractionation. This compound's structure determined spectroscopic analyses (FTIR/NMR/HRES-MS). Biological...

This study evaluates the effects of gingival fibroblasts, type I collagen and autocrine/paracrine elements on cytokine expression in paired primary metastatic human squamous cell carcinoma (HNSCC) lines. Additionally, IL-1alpha, IL-1beta, IL-6, TNF-alpha, TGF-beta HGF MMPs invasion were investigated. RT-PCR results indicated presence mRNAs for HNSCC lines but high cytokines was not a prerequisite cancer cells. mRNA detected Co-culturing cells with fibroblasts caused increases expression....

This work presents the multilayered caries model with a visuo-tactile virtual reality simulator and randomized controlled trial protocol to determine effectiveness of in training for minimally invasive removal. A three-dimensional, was reconstructed from 10 micro-computed tomography (CT) images deeply carious extracted human teeth before after The full grey scale 0-255 yielded median value 0-9, 10-18, 19-25, 26-52, 53-80 regarding dental pulp, infected dentin, affected normal enamel,...

Background: Dental caries commonly occurs during orthodontic treatment because fixed appliances can impede effective oral hygiene practices. This study investigated the effects of on dental biofilm maturity and virulence gene (gtfB, ldh, brpA, spaP, luxS, gbpB) expression. Methods: biofilms expression were determined in 24 patients before after ≥6 months. A three-tone disclosing gel was used to stain assess its by color change—pink (new biofilm), purple (mature light blue (cariogenic...

Genomic alteration in head and neck squamous cell carcinoma (HNSCC) was studied two line pairs (HN30-HN31 HN4-HN12) using conventional C-banding, multiplex fluorescence situ hybridization (M-FISH), array comparative genomic (array CGH). HN30 HN4 were derived from primary lesions the pharynx base of tongue, respectively, HN31 HN12 lymph-node metastatic belonging to same patients. Gain chromosome 1, 7, 11 shared almost all lines. Hierarchical clustering revealed that closely related HN4, which...

Mucin 1 (MUC1) is a transmembrane glycoprotein that contributes to the cellular response in hypoxic conditions different carcinomas. We investigated gene expression pattern of MUCs (1, 2, 4, 5AC, 5B, 6, 15, 16, and 19) isogenic primary (HN4 HN30) metastatic (HN12 HN31) head neck squamous cell carcinoma (HNSCC) lines. MUC1 was significantly up-regulated at mRNA protein levels HN12 HN31 cells, whereas, other exhibited diverse patterns between HNSCC Immunohistochemistry demonstrated exclusively...

Background and Objective Lipopolysaccharides ( LPS ) induce the production of proinflammatory mediators such as prostaglandins matrix metalloproteinases MMP s) in human gingival fibroblasts HGF s). Zingiber cassumunar is a medicinal plant that possesses anti‐inflammatory properties. The aim this study was to determine effects Z. extract on expression cyclooxygenase COX )‐1, ‐2 s challenged with . Material Methods HGFs were treated presence or absence extracts. levels COX‐1 , COX‐2 MMP‐2 mRNA...

House dust mite (HDM) induced matrix metalloproteinase (MMP)-9 plays a role in asthma. Zingiber cassumunar Roxb. (Phlai Thai) has been used folk medicine for asthma treatment.We investigated effects of Phlai and its constituent (E)-4-(3',4'-dimethoxyphenyl)but-3-en-1-ol (compound D) on the cleavage pro- MMP-9 by HDM. The these compounds phorbol 12-myristate 13-acetate (PMA)- gene protein expression airway epithelial cells (NCI-H292) were also investigated.Pro-MMP-9 was directly activated...

Macrolide antibiotics are effective drugs in chronic bronchiolitis and rhinosinusitis with mucus hypersecretion. However, the mechanism of action is unclear. This study was designed to investigate effect azithromycin (AZM; 15-membered) midecamycin acetate (MDM; 16-membered) on MUC5AC MUC2 gene expression secretion from human airway epithelial cells. The effects two macrolides tumor necrosis factor-α (TNF-α) release were also examined.Confluent NCI-H292 mucoepidermoid airways cells pretreated...

Zingiber cassumunar Roxb. has been used for traditional medicine, but few studies have described its potential toxicity. In this study, the acute and chronic oral toxicity of Z. extract granules were evaluated in Sprague-Dawley rats. The at a single dose 5000 mg/kg body weight did not produce treatment related signs or mortality any animals tested during 14-day observation period. However, decrease weights was observed treated males <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...