A5080225027- Burkholderia infections and melioidosis
- Mycobacterium research and diagnosis
- Insect symbiosis and bacterial influences
- Pharmacological Effects of Natural Compounds
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Chemical Looping and Thermochemical Processes
- Vibrio bacteria research studies
- Tuberculosis Research and Epidemiology
- Viral gastroenteritis research and epidemiology
- Legionella and Acanthamoeba research
- Brucella: diagnosis, epidemiology, treatment
- Animal Virus Infections Studies
- Ginseng Biological Effects and Applications
- Bacterial biofilms and quorum sensing
- Pancreatitis Pathology and Treatment
- SARS-CoV-2 and COVID-19 Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Receptor Mechanisms and Signaling
- Escherichia coli research studies
- Veterinary medicine and infectious diseases
- Complement system in diseases
- Yersinia bacterium, plague, ectoparasites research
- Pharmacology and Obesity Treatment
Roslin Institute
2014-2024
University of Edinburgh
2014-2024
The Pirbright Institute
2005-2010
University of Reading
2003-2008
United States Army Medical Research Institute of Infectious Diseases
2005
King's College London
2004
Summary Burkholderia pseudomallei is a Gram‐negative facultative intracellular pathogen that enters and escapes from eukaryotic cells using the power of actin polymerization. We have identified bacterial protein (BimA) required for ability B. to induce formation tails. BimA contains proline‐rich motifs WH2‐like domains shares limited homology at C‐terminus with Yersinia autosecreted adhesin YadA. located pole cell which polymerization occurs mutation bimA abolished actin‐based motility in...
ABSTRACT Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei . Here report that mallei and thailandensis are capable in J774.2 cells BimA homologs these bacteria can restore the defect B. bimA mutant. While differ their amino-terminal sequence, they interact directly with actin vitro vary ability to bind Arp3.
Legionnaires’ disease is a severe form of pneumonia caused by the environmental bacterium Legionella pneumophila. Outbreaks commonly affect people with known risk factors, but genetic and pathogenic complexity L. pneumophila within an outbreak not well understood. Here, we investigate etiology major that occurred in Edinburgh, UK, 2012, examining evolutionary history, genome content, virulence clinical isolates. Our high resolution genomic approach reveals was multiple subtypes pneumophila,...
Mycobacterium avium subspecies paratuberculosis (MAP) is the etiological agent of Johne's Disease, a chronic enteritis ruminants prevalent across world. It estimated that approximately 50% UK dairy herds are infected with MAP, but this likely an underestimate true prevalence. Infection can result in reduced milk yield, infertility and premature culling animal, leading to significant losses farming economy negatively affecting animal welfare. Understanding initial interaction between MAP host...
Integrin-linked kinase (ILK) has been implicated in the regulation of a range fundamental biological processes such as cell survival, growth, differentiation, and adhesion. In platelets ILK associates with beta1- beta3-containing integrins, which are paramount importance for function platelets. Upon stimulation this association integrins is increased activity up-regulated, suggesting that may be important coordination platelet responses. study conditional knockout mouse model was developed...
Burkholderia pseudomallei and mallei are closely related Gram-negative bacteria responsible for the infectious diseases melioidosis glanders, respectively. Autotransporters (ATs) comprise a large diverse family of secreted outer membrane proteins that includes virulence-associated invasins, adhesins, proteases, actin-nucleating factors. The B. K96243 genome contains 11 predicted ATs, eight which share homologs in ATCC 23344 genome. This review distils key findings from silico, vitro, vivo...
Species A rotavirus (RVA) vaccines based on live attenuated viruses are used worldwide in humans. The recent establishment of a reverse genetics system for rotoviruses (RVs) has opened the possibility engineering chimeric expressing heterologous peptides from other viral or microbial species order to develop polyvalent vaccines. We tested feasibility this concept by two approaches. First, we inserted short SARS-CoV-2 spike into hypervariable region simian RV SA11 strain protein (VP) 4....
ABSTRACT Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA. We report a high degree conservation bimA in 99 B. isolates from area endemicity. A geographically restricted subset harbored mallei -like allele (12.1%), confounding differential diagnostic test based on amplification species-specific regions.
Salmonella enterica serovar Typhimurium is an animal and zoonotic pathogen of worldwide importance. Intestinal colonization, induction enteritis systemic translocation by this bacterium requires type III protein secretion. Strategies that target process have the potential to control infection, pathology transmission. We defined global transcriptional response S. INP0403, a member family salicylidene acylhydrazides inhibit secretion (T3S). INP0403 treatment was associated with reduced...
Abstract Background Burkholderia pseudomallei is the causative agent of melioidosis where highest reported incidence world wide in Northeast Thailand, saline soil and water are prevalent. Moreover, recent reports indicate a potential pathogenic role for B. cystic fibrosis lung disease, an increased sodium chloride (NaCl) concentration airway surface liquid has been proposed. These observations raise possibility that high salinity may represent favorable niche . We therefore investigated...
Neutrophils play a key role in the control of Burkholderia pseudomallei, pathogen that causes melioidosis. Here, we show survival intracellular B. pseudomallei was significantly increased presence 3-methyladenine or lysosomal cathepsin inhibitors. The LC3-flux pseudomallei-infected neutrophils. Concordant with this result, confocal microscopy analyses using anti-LC3 antibodies revealed pseudomallei-containing phagosomes partially overlapped LC3-positive signal at 3 and 6 h postinfection....
Burkholderia pseudomallei is an intracellular pathogen and the causative agent of melioidosis, a severe disease humans animals. One virulence factors critical for early stages infection secretion apparatus (Bsa) Type 3 Secretion System (T3SS), molecular syringe that injects bacterial proteins, called effectors, into eukaryotic cells where they subvert cellular functions to benefit bacteria. Although Bsa T3SS itself known be important invasion, replication, virulence, only few genuine...
The intracellular pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans and various animals, is capable survival movement within cytoplasm host cells by a process known as actin-based motility. bacterial factor BimA required for motility through its direct interaction with actin, mediating actin polymerization at single pole bacterium to promote both between cells. However, little about other proteins this process. Here, we have investigated role bimC gene...
Burkholderia species use BimA for intracellular actin-based motility. Uniquely, thailandensis harbors a central and acidic (CA) domain. The CA domain was required motility, binding to the cellular Arp2/3 complex, Arp2/3-dependent polymerization of actin monomers. Our data reveal distinct strategies motility among species.
Enteropathogenic and enterohaemorrhagic Escherichia coli express a cell cycle-inhibiting factor (Cif), that is injected into host cells via Type III secretion system (T3SS) leading to arrest of division, delayed apoptosis cytoskeletal rearrangements. A homologue Cif has been identified in Burkholderia pseudomallei (CHBP; B. pseudomallei; BPSS1385), which shares catalytic activity, but its prevalence, function are ill-defined. Among 43 available genome sequences, 33 genomes (76.7%) harbor the...
Infection of ruminants such as cattle with Mycobacterium avium subsp . paratuberculosis (MAP) causes Johne’s disease, a disease characterized by chronic inflammation the small intestine and diarrhoea. MAP is acquired via faecal-to-oral route pathogen initially invades epithelial lining intestine. In this study we used an in vitro 3D mouse enteroid model to determine influence M cells infection gut epithelia MAP, comparison another bacterial intestinal veterinary importance, Salmonella...