A5009723414- Burkholderia infections and melioidosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eosinophilic Disorders and Syndromes
- Immune cells in cancer
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Microbial metabolism and enzyme function
- Complement system in diseases
- Immunotherapy and Immune Responses
- Pancreatitis Pathology and Treatment
Mahidol University
2018-2022
Associated Medical Services
2015
Medical Diagnostic Laboratories (United States)
2015
Khon Kaen University
2010-2015
ABSTRACT Burkholderia pseudomallei is the causative pathogen of melioidosis, which a major predisposing factor diabetes mellitus. Polymorphonuclear neutrophils (PMNs) kill microbes extracellularly by release neutrophil extracellular traps (NETs). PMNs play key role in control but involvement NETs killing B. remains obscure. Here, we showed that bactericidal were released from human response to dose- and time-dependent manner. -induced NET formation required NADPH oxidase activation not...
Neutrophils play a key role in the control of Burkholderia pseudomallei, pathogen that causes melioidosis. Here, we show survival intracellular B. pseudomallei was significantly increased presence 3-methyladenine or lysosomal cathepsin inhibitors. The LC3-flux pseudomallei-infected neutrophils. Concordant with this result, confocal microscopy analyses using anti-LC3 antibodies revealed pseudomallei-containing phagosomes partially overlapped LC3-positive signal at 3 and 6 h postinfection....
Neutrophil extracellular traps (NETs) are a recently identified, web-like, structure composed of decondensed nuclear DNA and associated antimicrobial granules. NETs extruded into the environment via reactive oxygen species (ROS)-dependent cell death pathway participating in inflammation autoimmune diseases. Transketolase (TKT) is thiamine pyrophosphate (vitamin B1)-dependent enzyme that links pentose phosphate with glycolytic by feeding excess sugar phosphates main carbohydrate metabolic...
Melioidosis is a severe infectious disease caused by the saprophytic facultative intracellular pathogen Burkholderia pseudomallei. The endemic in Southeast Asia and Northern Australia, no effective vaccine exists. To describe human cell-mediated immune responses to B. pseudomallei identify candidate antigens for development, ability of antigen-pulsed monocyte-derived dendritic cells (moDCs) trigger autologous T-cell its products was tested. moDCs were prepared from healthy individuals...
Lymphostatin is a virulence factor of enteropathogenic E. coli (EPEC) and non-O157 serogroup enterohaemorrhagic . Previous studies using whole-cell lysates EPEC showed that lymphostatin inhibits the mitogen-activated proliferation bulk human peripheral blood mononuclear cells (PBMCs) production cytokines IL-2, IL-4, IL-5, IFN-γ. Here, we used highly purified PBMC-derived T to show anti-CD3/anti-CD28-activated CD4 + CD8 blocks synthesis IL-10 IFN-γ without affecting cell viability in manner...