Background: The molecular basis of the RHD gene found in serologically D– phenotype individuals differs with race/ethnicity. Therefore, we aimed to develop a single-tube multiplex PCR-sequence specific primer (multiplex PCR-SSP) detect variant alleles commonly Thai population. Study design and methods: In total, 205 blood samples were tested using PCR-SSP targeted exons 1, 4, 7, 10, c.1227G>A exon 9 combination hybrid Rhesus box, results confirmed by direct DNA sequencing. Results:...

Allele frequencies (AFs) and haplotypic associations of human leukocyte antigen (HLA) class I II were investigated in 400 unrelated, healthy, ethnic Northeast Thais. HLA-A, -B, -Cw, -DRB1 -DQB1 typed by polymerase chain reaction-sequence specific primer, -sequence oligonucleotide probe -single-strand conformation polymorphism methods. In this population, 17 26 HLA-B, 15 HLA-Cw, HLA-DRB1 13 HLA-DQB1 alleles (or groups alleles) found. AFs > 10% included A*11 (23.3%), 24 (18.8%), 0207 (14.4%),...

HLA-associated relative risks of type 1 (insulin-dependent) diabetes mellitus were analysed in population-based Swedish patients and controls aged 0-34 years. The age dependence was assessed by likelihood ratio tests regression parameters separate logistic models for each HLA category. analyses demonstrated an attenuation with increasing at onset the risk positively associated DQB1*0201-A1*0502/B1*0302-A1*0301 (DQ2/8) genotype (P = 0.02) negatively DQB1*0602-A1*0102 (DQ6.2) haplotype 0.004)....

Purpose: To evaluate the sterility and safety of 100% nonpreserved, autologous, serum eye drop treatment in patients with ocular surface diseases. Methods: A total 147 autologous bottles (294 samples) from 21 diseases were included. Seven prepared for each patient, bottle was used only 1 day, then discarded. Two samples taken (before use after 24 hours use) cultured standard media. The culture plates held at least 6 weeks or until no growth could be confirmed. monitor safety, all admitted...

Hemoglobin E-β0 thalassemia and homozygous β0 -thalassemia are the most common chronic transfusion-dependent thalassemias in Thailand. Patients with these conditions can experience clinical complications such as RBC alloimmunization. In this study we aimed to determine prevalence, alloimmunization risk factors, antigenic exposure, evaluation of antigen- (C, c, E, e, Mia ) matched transfusion.Thalassemia patients were recruited from a tertiary care hospital for 10 years 2008 2017. The medical...

MICA or PERB11.1 is a polymorphic major histocompatibility complex (MHC) class I-related gene located 46 kb centromeric of the HLA-B in HLA I region. It expressed mainly gut epithelial cells, keratinocytes, endothelial fibroblasts and monocytes, upregulated by heat stress. has been found to interact with gamma delta T alpha beta CD8(+) natural killer (NK) cells bearing NKG2D/DAP10 receptor. The displays high degree polymorphism at least 54 alleles. In present study, exons 2, 3 4 were...

Abstract Background To evaluate the distributions of human leukocyte antigen (HLA) at class I and II loci that may contribute to genetic susceptibility psoriasis patients in north‐eastern Thai population. Materials methods We analyzed allelic frequencies HLA by using polymerase chain reaction‐amplification refractory mutation system (PCR‐ARMS) technique reaction‐single stranded conformation polymorphism (PCR‐SSCP), respectively, 140 Thais with were sudivided into two groups: one age onset...

Melioidosis is a severe infectious disease caused by the saprophytic facultative intracellular pathogen Burkholderia pseudomallei. The endemic in Southeast Asia and Northern Australia, no effective vaccine exists. To describe human cell-mediated immune responses to B. pseudomallei identify candidate antigens for development, ability of antigen-pulsed monocyte-derived dendritic cells (moDCs) trigger autologous T-cell its products was tested. moDCs were prepared from healthy individuals...

Abstract We here report the first study of antigen and phenotype frequencies Rh (C, c, E, e), M, Mi a Kidd antigens in north‐east Thai blood donors. Blood transfusion services aim to ensure availability adequate safe minimize development reactions. For pre‐transfusion testing, most important group systems are ABO RhD. The ABO‐compatible otherwise unknown may result alloimmunization, especially multi‐transfused patients. Extended red cell (RBC) phenotyping selection negative for specific...

Summary The expression of MICB, a member the major histocompatibility complex class I chain-related gene B family, is induced in response to cellular stress. It one ligands NKG2D receptor. MICB polymorphic, but distribution polymorphism north-eastern Thais and their potential associations with cancer have not yet been elucidated. In this study, polymerase chain reaction–sequence-specific primers were developed identify 15 alleles group alleles. We performed typing 100 healthy Thai females...

Psoriasis is a chronic inflammatory skin disorder. Although the aetiology and pathogenesis of psoriasis are unproven, it hypothesised that major histocompatibility complex (MHC) gene/haplotype contributes to susceptibility in many populations. MHC class I chain-related gene A (MICA), located 46-kb centromeric HLA-B, expressed on keratinocytes fibroblasts. MICA linkage disequilibrium with HLA-B involved natural killer-cell functions. To investigate relative contribution psoriasis,...

Abstract Cancer of the cervix is one common cancers among women worldwide. The primary risk factor cervical cancer high‐risk group human papillomavirus infection. Host genetic factors should also be involved. Major histocompatibility complex class I chain related A (MICA), a ligand to natural killer cell receptor (NKG)2D relevant immune surveillance, was investigated as potential candidate. MICA highly polymorphic. Although data were limited regarding functional polymorphism, it conceivable...

BACKGROUND : Platelet transfusions have been widely used in Thailand, but little is known about the phenotyping of human platelet antigens. STUDY DESIGN AND METHODS Whole blood was collected from 483 donors for preparation platelets. An improved mixed passive hemagglutination assay this study. RESULTS Frequencies demonstrated were 100 percent HPA‐1a (PlA1), 15.94 HPA‐ 2b (Siba), 60.25 HPA‐3a (Baka), 98.76 HPA‐4a (Yukb), 1.86 HPA‐4b (Yuka), 5.38 HPA‐5b (Br(a)), and 97.72 Naka. CONCLUSION...

Summary Polymorphic Alu insertions (POALINs) are known to contribute the strong polymorphic nature of Major Histocompatibility Complex (MHC). Previous population studies on MHC POALINs were limited only Australian Caucasians and Japanese. Here, we report individual insertion frequency five within class I region, their HLA‐A ‐B associations, three four locus alpha block POALIN haplotype frequencies in Northeastern (NE) Thai population. Of POALINs, lowest was 0.018 for AluyHF highest 0.292...

Psoriasis vulgaris, a common inflammatory skin disorder, is known to be associated with the HLA-Cw*06 allele. It has been recently suggested by microsatellite mapping that real susceptible gene for psoriasis resides in approximately 100-kb genomic region telomeric of HLA-C gene. In this respect, corneodesmosin (CDSN) 160-kb strong candidate because its location and functional role corneocyte cohesion desquamation. fact, significant association between CDSN polymorphism was recognized...

Anti-CD36s, developing after transfusion or during pregnancy, play an important role in immune-mediated bleeding disorders among Asian populations. Currently, little is known about the clinical relevance of anti-CD36. Here, we aimed to determine frequency CD36 deficiency Thais by analyzing expression on cell surfaces and plasma.The platelets monocytes were determined flow cytometry. Mutations gene analyzed nucleotide sequencing. Soluble (sCD36) plasma was quantified with enzyme-linked...

The phenotype and gene frequencies of HLA class II alleles were studied in the North‐eastern Thai population. Blood samples collected from 100 unrelated healthy Thais. HLA‐DRB1 DQB1 genes typed using polymerase chain reaction – sequence specific primer (PCR‐SSP) oligonucleotide probe (PCR‐SSO) techniques. Twenty‐six 11 found this DRB1 * 1202, 1502, 0405 0502/0504, 0301/0304 commonly found. Linkage disequilibrium analysis suggested existence 13 DR‐DQ haplotypes. 1502 ‐ 0501 haplotype was most...

Abstract Human leukocyte antigen (HLA)‐B*15 encompasses an increasing number of subtypes more than 150. Frequency studies and a strong genetic association between HLA susceptibility to drug hypersensitivity have been reported in different ethnic populations. To identify HLA‐B*15 Burmese using sequence‐based typing (SBT) method, we selected 65 ‐positive samples from 170 unrelated healthy who were genotyped HLA‐B * by polymerase chain reaction with the sequence‐specific primer method. The...

Abstract Human leukocyte antigens (HLA), class I, are a group of expressed on most nucleated cell surfaces. They transport endogenous peptides to the surface for recognition by T‐cell receptors. Their functions involved in immune responses. Many diseases associated with HLA alleles, especially HLA‐B*27 that is strongly ankylosing spondylitis (AS). consists 42 subtypes. Different subtypes were reported different ethnic groups AS patients. In this study, high‐resolution polymerase chain...