A5077861576- Trace Elements in Health
- Iron Metabolism and Disorders
- Molecular Sensors and Ion Detection
- Electrochemical Analysis and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- Lanthanide and Transition Metal Complexes
- X-ray Diffraction in Crystallography
- Drug Transport and Resistance Mechanisms
- Nanoplatforms for cancer theranostics
- Hemoglobinopathies and Related Disorders
- Crystallography and molecular interactions
- Graphene and Nanomaterials Applications
- Hemoglobin structure and function
- Autophagy in Disease and Therapy
- Magnetism in coordination complexes
- Barrier Structure and Function Studies
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Electrochemical sensors and biosensors
- Mass Spectrometry Techniques and Applications
- Nanomaterials for catalytic reactions
- Intracerebral and Subarachnoid Hemorrhage Research
- Advanced Electron Microscopy Techniques and Applications
Gifu Pharmaceutical University
2016-2025
Kyoto University
2006-2012
Although labile iron plays critical roles in diverse biological processes living cells, the physiological and pathophysiological functions of have not been sufficiently explored, partially due to a lack methods for visualizing intracellular iron. In this edge article, we present novel turn-on fluorescent probe (RhoNox-1) selective detection Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations electrochemical revealed that fluorescence quenching RhoNox-1...
Copper is an essential metal nutrient that tightly regulated in the body because loss of its homeostasis connected to severe diseases such as Menkes and Wilson diseases, Alzheimer’s disease, prion disorders, amyotrophic lateral sclerosis. The complex relationships between copper status various stages health disease remain challenging elucidate, part due a lack methods for monitoring dynamic changes pools whole living organisms. Here we present synthesis, spectroscopy, vivo imaging...
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free Cd2+-bound forms, respectively, dissociation constant 0.16 nM Cd2+. CadMQ is cell permeable locates within acidic compartments cells. further show that useful tool to ratiometrically change intracellular Cd2+ levels with use two excited wavelengths.
Hypoxia and acidity provide microenvironment for selection under evolutionary pressure proliferation in cancer cells. Carbonic anhydrases (CAs) are a superfamily of metalloenzymes present all life kingdoms, equilibrating the reactions among CO2, bicarbonate H+. CA9, membrane-associated α-CA, has been drug target various cancers. Whereas iron is essential not only cells but also lives on earth, little known association hypoxia, metabolism, extracellular redox regulation. Malignant...
Oxygen-dependent fluctuation of labile Fe(<sc>ii</sc>) was visualized by a new N-oxide-based fluorescent probe for ion.
Labile heme (LH) is a complex of Fe(II) and protoporphyrin IX, an essential signaling molecule in various biological systems. Most the subcellular dynamics LH remain unclear because lack efficient chemical tools for detecting cells. Here, we report activity-based fluorescence probe that can monitor fluctuations events. H-FluNox selective fluorescent senses using biomimetic N-oxide deoxygenation to trigger fluorescence. The selectivity >100-fold against Fe(II), enabling discrimination from...
Iron metabolism is closely associated with the pathogenesis of obesity. However, mechanism iron-dependent regulation adipocyte differentiation remains unclear. Here, we show that iron essential for rewriting epigenetic marks during differentiation. supply through lysosome-mediated ferritinophagy was found to be crucial early stage differentiation, and deficiency this period suppressed subsequent terminal This demethylation both repressive histone DNA in genomic regions...
A new class of high-contrast Fe(<sc>ii</sc>) selective fluorescent probes based on spirocyclized scaffolds were developed to visualize transferrin-delivered intracellular labile iron.
Abstract Non-thermal atmospheric pressure plasma is applicable to living cells and has emerged as a novel technology for cancer therapy. Plasma recently been shown affect not only by direct irradiation, but also indirect treatments with previously prepared plasma-activated medium (PAM). Iron an indispensable element potentially toxic because it generates the hydroxyl radical (•OH) in presence of hydrogen peroxide (H 2 O ) via Fenton reaction. The aim present study was demonstrate...
Iron is involved in numerous physiologically essential processes our body. However, excessive iron a pathogenic factor neurodegenerative diseases, causing aberrant oxidative stress. Divalent metal transporter 1 (DMT1) acts as primary of Fe(ii) ions. The intracellular delivery DMT1 toward the cellular membrane via trans-Golgi network during endocytotic process partially regulated by retromer-mediated protein-sorting system comprising vacuolar proteins (VPSs). Thus, together with DMT1,...
Abstract An efficient and simple method for the synthesis of various [1, 2, 3]triazolo[1, 5‐ a ]pyridines has been established. The involves copper(II)‐catalyzed oxidative NN bond formation that uses atmospheric oxygen as terminal oxidant following hydrazonation in one pot. use ethyl acetate solvent dramatically promotes bond‐formation reaction enables application cyclization one‐pot reaction. A mechanism was proposed on basis results spectroscopic study.
Abstract After intracranial hemorrhage (ICH), iron is released from the hematoma and induces secondary brain injury. However, detail effect of on blood-brain barrier (BBB) function still unknown. We investigated whether hemoglobin (Hb), ferrous ammonium sulfate (FAS) or hemin which contains have detrimental both human microvascular endothelial cells pericytes by cellular analysis in vitro . developed an (Fe 2+ )-detectable probe, Si-RhoNox-1, to investigate intracellular Fe accumulation...
Transition metals serve as an important class of micronutrients that are indispensable for bacterial physiology but cytotoxic when they in excess. Bacteria have developed exquisite homeostatic systems to control the uptake, storage, and efflux each biological maintain a thermodynamically balanced metal quota. However, whether pathways homeostasis different cross-talk render cross-resistance or sensitivity host-pathogen interface remains largely unknown. Here, we report zinc (Zn) excess...
Ferroptosis is an emerging type of cell death mode that dependent on iron. Unfortunately, the detailed analysis function organelle labile Fe(ii) in oxidative damage and lethality cells has not been demonstrated so far, mainly due to lack efficient methods visualize at targeted organelles. We have recently reported a series Fe(ii)-selective fluorescent probes, i.e., Ac-MtFluNox, Lyso-RhoNox, ER-SiRhoNox, which can detect specifically mitochondria, lysosomes, endoplasmic reticulum (ER),...
High-throughput methods for monitoring subcellular labile Fe(II) are important conducting studies on iron homeostasis and the discovery of potential drug candidates treatment deficiency or overload. Herein, a highly sensitive robust fluorescent probe detection intracellular is described. The was designed through rational optimization reactivity responsiveness an Fe(II)-induced fluorogenic reaction based deoxygenation N-oxide, which developed in-house. ready to use 96-well-plate-based...
Development of a turn-on and selective fluorescent probe for mitochondrial labile Fe(<sc>ii</sc>).
Iron is an essential transition metal species for all living organisms and plays various physiologically important roles on the basis of its redox activity; accordingly, disruption iron homeostasis triggers oxidative stress cellular damage. Therefore, cells have developed sophisticated iron-uptake machinery to acquire while protecting from uncontrolled damage during uptake process. To examine detailed mechanism controlling status, it necessary develop useful methods with state selectivity,...
Oral cancer accounts for ~2% of all cancers worldwide, and therapeutic intervention is closely associated with quality life. Here, we evaluated the effects non-thermal plasma on oral squamous cell carcinoma cells special reference to catalytic Fe(II). Non-thermal exerted a specific killing effect in comparison fibroblasts. Furthermore, was dependent amounts Fe(II), present especially lysosomes. After application, lipid peroxidation occurred peroxides mitochondrial superoxide were generated....
Ovarian endometriosis is a recognized risk for infertility and epithelial ovarian cancer, presumably due to iron overload resulting from repeated hemorrhage. To find clue early detection prevention of endometriosis-associated it mandatory evaluate catalytic (labile) ferrous (catalytic Fe(II)) study manipulation in endometriotic lesions. By the use tissues women as well endometrial tissue with without endometriosis, we first time performed histological analysis cellular Fe(II) specific...