Although labile iron plays critical roles in diverse biological processes living cells, the physiological and pathophysiological functions of have not been sufficiently explored, partially due to a lack methods for visualizing intracellular iron. In this edge article, we present novel turn-on fluorescent probe (RhoNox-1) selective detection Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations electrochemical revealed that fluorescence quenching RhoNox-1...

The synthesis of an extracellular ribbon cellulose in the bacterium Acetobacter xylinum takes place from linearly arranged, membrane-localized, cellulose-synthesizing and extrusion complexes that direct coupled steps polymerization crystallization. To identify different components involved this process, we isolated (acs) operon bacterium. Analysis DNA sequence shows presence three genes acs operon, which first gene (acsAB) codes for a polypeptide with molecular mass 168 kDa, was identified...

Cariogenic bacteria and periodontopathic are present in dental plaque as biofilms. In this study, we investigated the antibacterial effects of essential oils on following oral bacteria: Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Streptococcus mutans, sobrinus. We tested manuka oil, tea tree eucalyptus lavandula romarinus oil determined their minimum inhibitory concentration bactericidal concentration. The inhibited growth tested, being most...

This study assesses the diagnostic value of Lipiodol (iodized oil) and computed tomography (CT) in detecting hepatocellular carcinoma (HCC). Twenty-four patients who were suspected having HCC received injections a small amount Lipiodol, along with an antitumor agent, hepatic artery following routine celiac angiography. CT scans obtained 7-10 days after administration demonstrated distinct contrast to surrounding noncancerous parenchyma. In particular, CT-Lipiodol procedure disclosed many...

A DNA vaccine constructed with the CMV promoter conjugated to env gp160 and rev genes has been shown induce an effective Th1-type immune response when inoculated via intramuscular route. In present study, we obtained high levels of both humoral cell-mediated activity by intranasal administration this vaccine. The production mucosal IgA Ab in feces vaginal fluid was stimulated significantly administration. This route resulted a significant level HIV-1-neutralizing Abs serum. Cytokine assays...

The state excited from the ground singlet of ${\mathrm{Co}}^{3+}$ in ${\mathrm{LaCoO}}_{3}$ was investigated by submillimeter-wave electron spin resonance (ESR) measurements. Clear ESR signals with zero-field splitting were observed above 20 K for first time. activation energy is estimated to be $\ensuremath{\Delta}\ensuremath{\approx}140\mathrm{K},$ temperature dependence signal intensity and resonance-width. frequency angular successfully analyzed an effective spin-Hamiltonian uniaxial...

Magnetic resonance (MR) images of the liver were used to study 43 patients with relatively small hepatocellular carcinomas (HCCs) and 36 other hepatic mass lesions. In 27 HCC patients, histologic findings available. All focal lesions detectable by CT without contrast media delineated greater MR imaging. The rate detection depended on tumor size, being 97.5% for HCCs than 2 cm in longest axis 33.3% those less cm. imaging demonstrated ring sign characteristic encapsulated twice as frequently...

We developed a novel near-infrared (NIR) fluorescent probe, GPU-167, for in vivo imaging of tumor hypoxia. GPU-167 comprises tricarbocyanine dye as an NIR fluorophore and two 2-nitroimidazole moieties exogenous hypoxia markers that undergo bioreductive activation then selective entrapment hypoxic cells. After treatment with cells contained significantly higher levels fluorescence than normoxia. In specifically detected tumors 24 h after administration. Ex analysis revealed showed strong...

Oxygen-dependent fluctuation of labile Fe(<sc>ii</sc>) was visualized by a new N-oxide-based fluorescent probe for ion.

Cranberry juice is known to inhibit bacterial adhesion. We examined the inhibitory effect of cranberry on adhesion oral streptococci strains labeled with [ 3 H]‐thymidine saliva‐coated hydroxyapatite beads (s‐HA). When cells were momentarily exposed juice, their adherence s‐HA decreased significantly compared control ( P &lt; 0.01). Their hydrophobicity also dependently concentration juice. evaluated biofilm formation. By using a microplate system, we found that high molecular mass...

The accuracy of the duplex Doppler ultrasound system in measurement blood flow splenic artery and superior mesenteric was evaluated seven anesthetized dogs by comparing recordings obtained simultaneously with electromagnetic flowmeter, those combination B-mode M-mode scanning. Various rates were produced infusion dopamine. Splenic measured exhibited significant correlations corresponding values measurements (r = .93 r .93, respectively). Changes splanchnic circulation progression chronic...

Serum and gingival crevicular fluid from normal healthy adults patients with periodontitis were screened for immunoglobulin G antibodies to antigens Bacteroides gingivalis 381, intermedius 24, loescheii ATCC 15930, Fusobacterium nucleatum 25586, Eikenella corrodens 1073, Actinobacillus actinomycetemcomitans 29522, Capnocytophaga sp. strain M-12. Immunoglobulin antibody titers the measured by an enzyme-linked immunosorbent assay. The levels B. in serum significantly higher samples than...

The hemagglutinating factor (hemagglutinin) of Bacteroides gingivalis was prepared from the supernatant a 5-day diffusate broth culture by ammonium sulfate precipitation and column chromatography with hydrophobic Phenyl-Sepharose CL-4B, DEAE-Sephadex A-50, Sephadex G-100 gel filtration. activity preparation 53.3 times higher than that precipitate. In electron microphotographs, hemagglutinin appears to have vesicle or tubelike structure. intact cells completely destroyed heating at 100...

Microorganisms are able to survive and induce persistent infection in periapical tissues. The aim of this study was investigate the composition microflora apical periodontitis lesions.Twenty lesion samples were obtained from 20 patients with chronic by root end surgery processed using aerobic or anaerobic culture techniques. All isolated strains identified 16S ribosomal DNA sequence analysis.Seventy-four isolated, belonging 31 bacterial species lesions that isolated. majority facultative...

A new class of high-contrast Fe(<sc>ii</sc>) selective fluorescent probes based on spirocyclized scaffolds were developed to visualize transferrin-delivered intracellular labile iron.

Abstract An efficient and simple method for the synthesis of various [1, 2, 3]triazolo[1, 5‐ a ]pyridines has been established. The involves copper(II)‐catalyzed oxidative NN bond formation that uses atmospheric oxygen as terminal oxidant following hydrazonation in one pot. use ethyl acetate solvent dramatically promotes bond‐formation reaction enables application cyclization one‐pot reaction. A mechanism was proposed on basis results spectroscopic study.

Summary Localization of Porphyromonas gingivalis and Treponema denticola in different areas subgingival plaque from advanced adult periodontitis patients was studied immunohistochemically using sensitive immunogold‐silver staining immunoelectron microscopy. Fourteen periodontally diseased teeth were extracted without damaging the plaque, fixed, embedded. The samples sectioned according to four pocket depths (0–2 mm, 2–4 4–6 mm ≤6 mm). Serial thin sections stained specific antisera P. or T....

A new species, Porphyromonas gulae sp. nov., is proposed to include strains isolated from the gingival sulcus of various animal hosts which are distinct related gingivalis human origin. This bacterium exhibits following characteristics: black-pigmented colonies; asaccharolytic, obligate anaerobic growth; and Gram-negative, non-motile non-spore-forming, rod-shaped cells. Colonies do not fluoresce under UV light. Vitamin K1 haemin required for growth. Cells haemagglutinate sheep erythrocytes....

T. Hamano, K. Okuda, Mashino, M. Hirobe, Arakane, A. Ryu, S. Mashiko and Nagano, Chem. Commun., 1997, 21 DOI: 10.1039/A606335G