A5102003507- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- RNA modifications and cancer
- Probiotics and Fermented Foods
- Graphene and Nanomaterials Applications
- Digestive system and related health
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Gut microbiota and health
- Medicinal Plant Pharmacodynamics Research
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Advanced oxidation water treatment
- PI3K/AKT/mTOR signaling in cancer
- Polymer Surface Interaction Studies
- Chronic Lymphocytic Leukemia Research
- Cancer-related gene regulation
- Seaweed-derived Bioactive Compounds
- Cancer Mechanisms and Therapy
Shanghai Jiao Tong University
2020-2025
Shanghai Sixth People's Hospital
2024-2025
Jilin Medical University
2025
Stomatology Hospital
2024
Jilin University
2024
Osaka University
2003-2024
XinHua Hospital
2024
China University of Mining and Technology
2024
Shanghai Ninth People's Hospital
2020-2023
Hangzhou Medical College
2018-2023
Previous studies have found that probiotic fermented camel milk has anti-diabetic effect by inducing (glucagon-like peptide-1) GLP-1 secretion. Probiotics are valuable in prevention and treatment of diabetes. As a result, our team islolated 14 probiotics from milk. These beneficial characteristics, but the possible mechanisms remains unclear. The present study aimed to explore possoble probiotics. C57BL/Ks mice were normal group. db/db randomized into five groups: model group, metformin...
Critical-sized bone defect repair in patients with diabetes mellitus remains a challenge clinical treatment because of dysfunction macrophage polarization and the inflammatory microenvironment region. Three-dimensional (3D) bioprinted scaffolds loaded live cells bioactive factors can improve cell viability further accelerating repair. Here, we used modified bioinks comprising gelatin, gelatin methacryloyl (GelMA), 4-arm poly (ethylene glycol) acrylate (PEG) to fabricate 3D containing BMSCs,...
The favorable microstructure and bioactivity of tissue-engineered bone scaffolds are closely associated with the regenerative efficacy defects. For treatment large defects, however, most them fail to meet requirements such as adequate mechanical strength, highly porous structure, excellent angiogenic osteogenic activities. Herein, inspired by characteristics a "flowerbed", we construct short nanofiber aggregates-enriched dual-factor delivery scaffold via 3D printing electrospinning...
Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal must provide physical guidance recruit cells and immune regulation improve regenerative microenvironment. This study presents a novel scaffold composed dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue...
Diagnostic tools for neurodegenerative diseases such as Alzheimer's disease (AD) currently involve subjective neuropsychological testing and specialized brain imaging techniques. While definitive diagnosis requires a pathological evaluation at autopsy, changes are believed to begin years before the clinical presentation of cognitive decline. Therefore, there is an essential need reliable biomarkers aid in early detection order implement preventative strategies. microRNAs (miRNA) small...
TFEB (transcription factor EB) is a master regulator of lysosomal biogenesis, function and autophagy. The transcriptional activity mainly controlled by its phosphorylation status mediated the MTOR (mechanistic target rapamycin [serine/threonine kinase]) complex 1 (MTORC1). At present, little known whether other forms posttranslational modifications (PTMs) such as acetylation also affects activity. In this study, we first observed that well-established histone deacetylase inhibitor...
Crosstalk between nerves and bone is essential for repair, which Schwann cells (SCs) are crucial in the regulation of microenvironment. Considering that exosomes critical paracrine mediators intercellular communication exert important effects tissue aim this study to confirm function molecular mechanisms cell-derived (SC-exos) on regeneration propose engineered constructs simulate SC-mediated nerve–bone crosstalk. SCs promoted proliferation differentiation marrow mesenchymal stem (BMSCs)...
Abstract Epithelial to mesenchymal transition (EMT) plays a crucial role in cancer metastasis, accompanied with vast epigenetic changes. AMP-activated protein kinase (AMPK), cellular energy sensor, regulatory roles multiple biological processes. Although few studies have shed light on AMPK regulating the inside mechanisms remain unknown. Herein we show that activation by metformin relieves repressive H3K9me2-mediated silencing of epithelial genes (e.g., CDH1) during EMT processes and...
Abstract The periosteum is a connective tissue membrane adhering to the surface of bone that primarily provides nutrients and regulates osteogenesis during development injury healing. However, building an artificial with good adhesion properties satisfactory for defect repair remains challenge, especially using three-dimensional (3D) bioprinting. In this study, dopamine was first grafted onto molecular chain gelatin N -(3-dimethylaminopropyl)- ′-ethylcarbodiimide hydrochloride...
Abstract Precise fabrication of microscale vasculatures (MSVs) has long been an unresolved challenge in tissue engineering. Currently, light‐assisted printing is the most common approach. However, this approach often associated with intricate process, high cost, and a requirement for specific photoresponsive materials. Here, thermoresponsive hydrogels are employed to induce volume shrinkage at 37 °C, which allows MSV engineering without complex protocols. The hydrogel consists...
Abstract The integration of three-dimensional (3D) bioprinted scaffold’s structure and function for critical-size bone defect repair is immense significance. Inspired by the basic component innate cortical tissue—osteons, many studies focus on biomimetic strategy. However, complexity hierarchical microchannels in osteon, requirement mechanical strength bone, biological angiogenesis osteogenesis remain challenges fabrication osteon-mimetic scaffolds. Therefore, we successfully built mimetic...
Limited infiltration and activity of natural killer (NK) T cells within the tumor microenvironment (TME) correlate with poor immunotherapy responses. Here, we examined role endonuclease Regnase-1 on NK cell anti-tumor activity. cell-specific deletion (Reg1ΔNK) augmented cytolytic interferon-gamma (IFN-γ) production in vitro increased intra-tumoral accumulation Reg1ΔNK-NK vivo, reducing growth dependent IFN-γ. Transcriptional changes included elevated IFN-γ expression, effectors, chemokine...
In the Kazakh community of Xinjiang, China, fermented camel milk has been traditionally used to manage diabetes. This study evaluates effects composite probiotics derived from (CPCM) on metabolic disturbances in a rat model Type 2 diabetes (T2DM). T2DM was induced Wistar rats using streptozotocin. Experimental groups included diabetic control, Metformin, and low- high-dose CPCM. Measurements over 6 weeks body weight (BW), fasting blood glucose (FBG), oral tolerance test (OGTT), glycated...
Leukocyte infiltration during inflammation is mediated by the sequential actions of adhesion molecules and chemokines. By using a rat ureteral obstruction model, we showed previously that L-selectin plays an important role in leukocyte into kidney. Here report purification, identification, characterization L-selectin-binding heparan sulfate proteoglycan (HSPG) expressed Partial amino acid sequencing Western blotting analyses HSPG collagen XVIII, basement membrane HSPG. The binding to...
Significance Idiopathic inflammatory bowel disease (IBD) is associated with the gut microbiota and immune system of host; however, precise pathogenesis IBD poorly defined. We show that specific deletion endoribonuclease Regnase-1 in intestinal epithelial cells relieves symptoms experimental colitis during acute inflammation. deficiency potentiates mTOR signaling purine metabolism colon epithelium. These data provide insight into role IBD.
Efficient healing of bone defect is closely associated with the structured and functional characters tissue engineered scaffolds. However, development implants rapid ingrowth favorable osteoinductive properties remains a challenge. Herein, we fabricated polyelectrolytes modified-biomimetic scaffold macroporous nanofibrous structures as well simultaneous delivery BMP-2 protein trace element strontium. The hierarchically incorporated strontium-substituted hydroxyapatite (SrHA) was coated...