A5022826938- Plant Molecular Biology Research
- Renal cell carcinoma treatment
- Renal and related cancers
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Plant nutrient uptake and metabolism
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Nephrotoxicity and Medicinal Plants
- RNA modifications and cancer
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Power Systems and Technologies
- Protein Degradation and Inhibitors
- Photosynthetic Processes and Mechanisms
- Cancer-related gene regulation
- Evaluation and Optimization Models
- Pancreatic and Hepatic Oncology Research
- Immune Cell Function and Interaction
Duke-NUS Medical School
2017-2025
SingHealth
2022-2025
National Cancer Centre Singapore
2023-2024
Sun Yat-sen University
2023
Sun Yat-sen University Cancer Center
2023
Yale University
2018-2020
South China University of Technology
2018-2020
Institute of Molecular and Cell Biology
2020
Global Energy Interconnection Research Institute North America
2019
National University of Singapore
2013-2017
Abstract Protein-coding mutations in clear cell renal carcinoma (ccRCC) have been extensively characterized, frequently involving inactivation of the von Hippel–Lindau (VHL) tumor suppressor. Roles for noncoding cis-regulatory aberrations ccRCC tumorigenesis, however, remain unclear. Analyzing 10 primary tumor/normal pairs and 9 lines across 79 chromatin profiles, we observed pervasive enhancer malfunction ccRCC, with cognate enhancer-target genes associated tissue-specific aspects...
Cholangiocarcinomas (CCA) pose a complex challenge in oncology due to diverse etiologies, necessitating tailored therapeutic approaches. This review discusses the risk factors, molecular pathology, and current options for CCA explores emerging strategies encompassing targeted therapies, immunotherapy, novel compounds from natural sources, modulation of gut microbiota. are driven by an intricate landscape genetic mutations, epigenetic dysregulation, post-transcriptional modification, which...
Peritoneal metastasis (PM) in gastric cancer (GC) is associated with poor prognosis and significant morbidity. We sought to understand the genomic, transcriptomic, tumor microenvironment (TME) features that contribute peritoneal organotropism GC.
A decade of studies on middle cortex (MC) formation in the root endodermis Arabidopsis (Arabidopsis thaliana) have revealed a complex regulatory network that is orchestrated by several GRAS family transcription factors, including SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE3 (SCL3). However, how their functions are regulated remains obscure. Here we show mutations SEUSS (SEU) gene led to higher frequency MC formation. seu mutants had strongly reduced expression SHR, SCR, SCL3,...
Prevalent copy number alteration is the most prominent genetic characteristic associated with ovarian cancer (OV) development, but its role in immune evasion has not been fully elucidated. In this study, we identified RAD21, a key component of cohesin complex, as frequently amplified oncogene that could modulate response OV. Through interrogating RAD21-regulated transcriptional program, found RAD21 directly interacts YAP/TEAD4 corepressors and recruits NuRD complex to suppress interferon...
Abstract Background Enhancer of zeste homolog 2 (EZH2), the key catalytic subunit polycomb repressive complex (PRC2), is overexpressed and plays an oncogenic role in various cancers through catalysis-dependent or catalysis-independent pathways. However, related mechanisms contributing to ovarian cancer (OC) are not well understood. Methods The levels EZH2 H3K27me3 were evaluated 105 OC patients by immunohistochemistry (IHC) staining, these stratified based on levels. Canonical noncanonical...
Objectives Cholangiocarcinoma (CCA) is a heterogeneous malignancy with high mortality and dismal prognosis, an urgent clinical need for new therapies. Knowledge of the CCA epigenome largely limited to aberrant DNA methylation. Dysregulation enhancer activities has been identified affect carcinogenesis leveraged therapies but uninvestigated in CCA. Our aim identify potential therapeutic targets different subtypes through profiling. Design Integrative multiomics activity profiling diverse was...
Abstract Poly (ADP‐ribose) Polymerase inhibitors (PARPi) have demonstrated remarkable clinical efficacy in treating ovarian cancer (OV) with BRCA1/2 mutations. However, drug resistance inevitably limits their applications and there is an urgent need for improved therapeutic strategies to enhance the utility of PARPi, such as Olaparib. Here, compelling evidence indicates that sensitivity PARPi associated cell cycle dysfunction. Through high‐throughput screening a kinase inhibitor library,...
Point mutations in the ligand binding domain of retinoic acid receptor alpha (RARα) are linked to breast fibroepithelial tumor development, but their role solid tumorigenesis is unclear. In this study, we assessed functional effects known RARα on signaling using biochemical and cellular assays. All tested mutants exhibited reduced transcriptional activity compared wild-type showed a dominant negative effect, feature associated with developmental defects formation. X-ray crystallography...
Malaria poses a significant health burden to endemic countries, necessitating the development of new antimalarials with novel mechanisms action combat emerging drug resistance. However, target identification methods for antimalarial compounds selected through phenotypic screening are currently limited, which has been major obstacle in field discovery. Here, we aim address this need using perturbation transcriptomics efficiently select targeting pathways or molecules. The method involves...
Cholangiocarcinoma (CCA) is a highly lethal hepatobiliary malignancy, with prognosis influenced by anatomical subtypes and etiological factors. This study successfully established three CCA cell lines: KKU-097, KKU-466, KKU-610, from the primary tumors of patients in liver fluke-endemic areas. These cells represent perihilar (pCCA) intrahepatic (iCCA) subtypes. Comprehensive analyses, including histopathology, molecular profiling, biomarkers, cancer phenotype characterization, drug...
Abstract Novel strategies to treat late-stage nasopharyngeal carcinoma that often develop resistance chemotherapy remains an unmet clinical demand. In this study, we identify the multi-kinase inhibitor pacritinib as capable of resensitizing response paclitaxel in acquired model. Transcriptome analysis paclitaxel-sensitive and -resistant cell lines, well chemorefractory samples, identified S100A9 top candidate gene suppressed by whose overexpression was significantly associated with poor...
The Lugol's staining method has been widely used to detect changes in the maintenance of stem cell fate columella root cap Arabidopsis roots since late 1990s. However, various limitations this demand for additional or complementary new approaches. For instance, it is unable reveal division rate cells. Here we report that, by labeling dividing cells with 5-ethynyl-2'-deoxyuridine (EdU), number and distribution their labeled progeny can be studied so that measured quantitatively addition,...
The circadian clock relies on regulated degradation of proteins to maintain rhythmicity. Despite this, we know few components that mediate protein degradation. This is due high levels functional redundancy within plant E3 ubiquitin ligase families. In order overcome this issue and discover ligases control function, generated a library transgenic Arabidopsis plants expressing dominant-negative 'decoy' ligases. We determined their effects the identified dozens new potential regulators...
Abstract Background Natural killer/T-cell lymphoma (NKTL) is a rare type of aggressive and heterogeneous non-Hodgkin's (NHL) with poor prognosis limited therapeutic options. Therefore, there an urgent need to exploit potential novel targets for the treatment NKTL. Histone deacetylase (HDAC) inhibitor chidamide was recently approved treating relapsed/refractory peripheral T-cell (PTCL) patients. However, its efficacy in NKTL remains unclear. Methods We performed phase II clinical trial...
Loss of ferroptosis contributes to the development human cancer, and restoration has been demonstrated as a potential therapeutic strategy in cancer treatment. However, mechanisms how escape ovarian (OV) are not well elucidated. Here, we show that negative regulation signatures correlated with tumorigenesis OV were associated poor prognosis, suggesting represents OV. High-throughput drug screening kinase inhibitor library identified MEK inhibitors inducers cells. We further...
TOPOISOMERASE1 (TOP1), which releases DNA torsional stress generated during replication through its relaxation activity, plays vital roles in animal and plant development. In Arabidopsis (Arabidopsis thaliana), TOP1 is encoded by two paralogous genes (TOP1α TOP1β), of TOP1α displays specific developmental functions that are critical for the maintenance shoot floral stem cells. Here, we show different populations root cells also dependent on TOP1α-specific functions, exerted distinct novel...
Acquired resistance to chemotherapy is one of the major causes mortality in advanced nasopharyngeal carcinoma (NPC). However, effective strategies are limited and underlying molecular mechanisms remain elusive. In this study, through transcriptomic profiling analysis 23 tumor tissues, we found that NOTCH3 was aberrantly highly expressed chemoresistance NPC patients, with overexpression being positively associated poor clinical outcome. Mechanistically, using an established cellular model,...
Diffuse large B-cell lymphoma (DLBCL) exhibits frequent inactivating mutations of the histone acetyltransferase CREBBP, highlighting attractiveness targeting CREBBP deficiency as a therapeutic strategy. In this study, we demonstrate that chidamide, novel deacetylase (HDAC) inhibitor, is effective in treating subgroup relapsed/refractory DLBCL patients, achieving an overall response rate (ORR) 25.0% and complete (CR) 15.0%. However, clinical to chidamide remains poor, most patients exhibit...
Abstract The ubiquitin proteasome system plays an essential role in the regulation of flowering time, a precisely timed developmental transition necessary for plant fitness. However, full impact... Targeted degradation proteins is mediated by E3 ligases and important execution many biological processes. Redundancy has prevented genetic characterization plants. Here, we performed reverse screen Arabidopsis using library dominant-negative U-box-type to identify their roles time reproductive...