A5082583928- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Genomics and Chromatin Dynamics
- Agronomic Practices and Intercropping Systems
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant pathogens and resistance mechanisms
- RNA Research and Splicing
- Seed Germination and Physiology
- GABA and Rice Research
- Bone health and osteoporosis research
- Genomics and Phylogenetic Studies
Chinese University of Hong Kong
2015-2024
Yale University
2018-2024
Chinese University of Hong Kong, Shenzhen
2022-2023
Prince of Wales Hospital
1999-2017
Inner Mongolia Agricultural University
2007
Biogen (United States)
2007
University of California, Davis
2002
The People's Hospital of Guangxi Zhuang Autonomous Region
1999
Quadram Institute
1992
John Innes Centre
1992
Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic have been lost during the domestication process. Here we sequence and assemble draft genome of soybean construct recombinant inbred population genotyping-by-sequencing phenotypic analyses identify multiple QTLs relevant traits interest agriculture. We use combination de novo sequencing data...
Abstract Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome wild soybean accession W05, with final assembled size 1013.2 Mb and contig N50 3.3 Mb. The analytical power W05 is demonstrated by several examples. First, identify an inversion at locus determining seed coat color during domestication. Second, translocation event between chromosomes 11 13 some genotypes shown to...
Pectin is a complex polysaccharide of great importance to food, pharmaceutical and cosmetic industries. It widely used in the food industry as thickener, gelling agent an emulsifier among others. also exhibits potential anti-inflammatory immunomodulatory effects. Its functional properties are determined by characteristics such neutral sugar side chains, molecular weight degree methyl-esterification. Human beings unable digest pectin therefore pectinases catalyze degradation pectic...
To obtain a comprehensive understanding of transcriptomic reprogramming under salt stress, we performed whole-transcriptome sequencing on the leaf and root soybean seedlings subjected to treatment in time-course experiment (0, 1, 2, 4, 24, 48 hr). This time series dataset enabled us identify important hubs connections gene expressions. We highlighted analysis phytohormone signaling pathways their possible crosstalks. Differential expressions were also found among those genes involved carbon...
Abstract Background In plants, HIR ( H ypersensitive I nduced R eaction) proteins, members of the PID (Proliferation, on and D eath) superfamily, have been shown to play a part in development spontaneous hypersensitive response lesions leaves, reaction pathogen attacks. The levels proteins were correlate with localized host cell deaths defense responses maize barley. However, not much was known about rice. Since rice is an important cereal crop consumed by more than 50% populations Asia...
Abstract ZEITLUPE (ZTL), a photoreceptor with E3 ubiquitin ligase activity, communicates end-of-day light conditions to the plant circadian clock. It still remains unclear how ZTL protein accumulates in but does not destabilize target proteins before dusk. Two deubiquitylating enzymes, UBIQUITIN-SPECIFIC PROTEASE 12 and 13 (UBP12 UBP13), which regulate clock period ubiquitylation manner opposite ZTL, associate complex. Here we demonstrate that interacting partner, GIGANTEA (GI), recruits...
Eukaryotic circadian clocks utilize the ubiquitin proteasome system to precisely degrade clock proteins. In plants, F-box-type E3 ligases ZEITLUPE (ZTL), FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1), and LOV PROTEIN2 (LKP2) regulate period couple photoperiodic flowering in response end-of-day light conditions. To better understand their functions, we expressed decoy ZTL, FKF1, LKP2 proteins that associate with target but are unable ubiquitylate targets Arabidopsis (Arabidopsis thaliana)....
The anionic toxicity of plants under salt stress is mainly caused by chloride (Cl-). Thus Cl- influx, transport and their regulatory mechanisms should be one the most important aspects plant tolerance studies, but are often sidelined focus on sodium (Na+) its associated adaptations. Plant channels (CLCs) proteins for anions including nitrate (NO3-), critical nutrition uptake transport, adjustment cellular turgor, stomatal movement, signal transduction, NO3- homeostasis stress.Among eight...
Long non-coding RNAs (lncRNAs) are defined as non-protein-coding transcripts that at least 200 nucleotides long. They known to play pivotal roles in regulating gene expression, especially during stress responses plants. We used a large collection of in-house transcriptome data from various soybean (Glycine max and Glycine soja) tissues treated under different conditions perform comprehensive identification lncRNAs. also retrieved publicly available were sufficient quality sequencing depth...
Plants that have experienced certain abiotic stress may gain tolerance to a similar in subsequent exposure. This phenomenon, called priming, was observed here soybean (Glycine max) seedlings exposed salt stress. Time-course transcriptomic profiles revealed distinctively different transcriptional responses the primed from those non-primed under high salinity stress, indicating response strategy of repressing unhelpful biotic and focusing on promotion important for tolerance. To identify...
The capability of a plant to protect itself from stress-related damages is termed "adaptability" and the phenomenon showing better performance in subsequent stress "stress memory". While drought one most serious disasters result climate change, current understanding priming soybean still inadequate for effective crop improvement. To fill this gap, study, memory response was evaluated cultivated (Glycine max). determine if prior would be beneficial survival soybean, plants were divided into...
Soybean yield is largely dependent on growth period. We characterized two period quantitative trait loci, Gp11 and Gp12, from a recombinant inbred population generated cross of wild (W05) cultivated (C08) soybean. Lines carrying Gp11C08 Gp12C08 tend to have shorter higher expression GmFT2a GmFT5a. Furthermore, multiple interval mapping suggests that Gp12 may be genetically interacting with the E2 locus. This consistent observation GmFT5a are activated by at ZT4 in recessive e2 but not...
Copy number variations (CNVs) play important roles in crop domestication. However, there is only very limited information on the involvement of CNVs soybean Trailing growth and long shoots are adaptations for natural habitats but cause lodging that hampers yield cultivation. Previous studies have focused Dt1/2 affecting indeterminate/determinate habit, whereas possible role gibberellin pathway remained unclear. In present study, quantitative trait locus (QTL) mapping a recombinant inbred...
Abstract In the face of global food security crises, it is necessary to boost agricultural production. One factor hampering attempts increase production elevated soil salinity, which can be due salt that naturally present in or a consequence excessive prolonged irrigation application fertiliser. response environmental stresses, plants activate multiple molecular mechanisms, including timely activation stress‐responsive transcriptional networks. However, case stress, combined effects initial...
The three main isoforms of the 19-kDa lipid body proteins (oleosin) have been purified to homogeneity from embryos rapeseed.The secondary structures these as derived circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy were compared with predicted primary sequences.The salient feature sequence all oleosins is its division into defined structural domains: a central hydrophobic domain flanked on either side by relatively hydrophilic domains, respectively.Using variety...
Preferential accumulation of transposable elements (TEs), particularly long terminal repeat retrotransposons (LTR-RTs), in recombination-suppressed pericentromeric regions seems to be a general pattern TE distribution flowering plants. However, whether such was formed primarily by preferential insertions into or selection against euchromatin remains obscure. We recently investigated 31 resequenced wild and cultivated soybean (Glycine max) genomes detected 34,154 unique nonreference mappable...
Abstract Phosphorylation of eIF2α is an important strategy for living organisms to adapt metabolic and physiological changes that are often associated with external stimuli. GCN2 one the well‐studied kinases in yeast mammals, which responsible survival organism under amino acid starvation. Despite downstream reactions being quite divergent, AtGCN2 exhibits a high primary sequence similarity its animal counterparts. In this study, we provide experimental evidence show shares similar...