A5020384260- Cardiomyopathy and Myosin Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- SARS-CoV-2 and COVID-19 Research
- Allergic Rhinitis and Sensitization
- Signaling Pathways in Disease
- Soybean genetics and cultivation
- Marine Invertebrate Physiology and Ecology
- Cancer-related gene regulation
- Selenium in Biological Systems
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Mitochondrial Function and Pathology
- Metal complexes synthesis and properties
- Mesenchymal stem cell research
- Venomous Animal Envenomation and Studies
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
Chinese University of Hong Kong
2014-2024
Sun Yat-sen University
2011
Sun Yat-sen University Cancer Center
2011
Agrobiotechnology Institute
2011
Victoria University of Wellington
2010
Prince of Wales Hospital
2006-2010
National Health Research Institutes
2010
University of Hong Kong
2001-2009
Hong Kong Polytechnic University
2009
University of Macau
2009
Exosomes are increasingly recognized as important mediators of cell-cell communication in cancer progression through the horizontal transfer RNAs and proteins to neighboring or distant cells. Hepatocellular carcinoma (HCC) is a highly malignant cancer, whose metastasis largely influenced by tumor microenvironment. The possible role exosomes interactions between HCC cell its surrounding hepatic milieu however unknown. In this study, we comprehensively characterized exosomal RNA proteome...
Protein methylation is one type of reversible post-translational modifications (PTMs), which plays vital roles in many cellular processes such as transcription activity, DNA repair. Experimental identification sites on proteins without prior knowledge costly and time-consuming. In silico prediction might not only provide researches with information the candidate for further determination, but also facilitate to perform downstream characterizations site-specific investigations. present study,...
The ensemble-based feature selection method presents the merit of acquisition more informative and compact features than those obtained by individual methods.
Plants that grow in extreme environments represent unique sources of stress-resistance genes and mechanisms. Ammopiptanthus mongolicus (Leguminosae) is a xerophytic evergreen broadleaf shrub native to semi-arid desert regions; however, its drought-tolerance mechanisms remain poorly understood. Here, we report the assembly reference-grade genome for A. mongolicus, describe evolutionary history within legume family, examine The assembled 843.07 Mb length, with 98.7% sequences successfully...
Increased serum haptoglobin concentration and changes in its glycosylation have been reported certain cancer types. Information for hepatocellular carcinoma (HCC) has not yet available. In this study, we aimed to carry out a systematic analysis of concentrations (Hp) glycoforms the patients with HCC noncancer only chronic liver diseases (CLD) examine their clinical values. This study was divided into two major parts, (1) measurement Hp concentration, investigation value diagnosis HCC, (2)...
Abstract Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) sources of mesenchymal stem cells (MSC) for cell therapy, with both UC‐ P‐MSC possess immunophenotypic functional characteristics similar BM‐MSC. However, their migration capacity, which is indispensable during tissue regeneration process, unclear. Under defined conditions, the capacity BM‐ was found 5.9‐ 3.2‐folds higher than that UC‐MSC, respectively. By use 2‐DE combined MS MS/MS...
Abstract Esophageal cancer (EC) persists to be a leading cancer‐related death in northern China. Clinical outcome of EC is the most dismal among many types digestive tumors because at early stage asymptomatic. The current study used 2‐DE‐based proteomics identify differentially expressed proteins between esophageal cell lines and immortal line. Fifteen were identified with differences more than five folds, comprising down‐regulation annexin A2, histone deacetylase 10 isoform beta protein...
Metabolomics is developing as an important functional genomics tool for understanding plant systems' response to genetic and environmental changes. Here, we characterized the metabolic changes of cultivated soybean C08 (Glycine max L. Merr) wild W05 soja Sieb.et Zucc.) under salt stress using MS-based metabolomics, in order reveal phenotypes their eight hybrid offspring (9H0086, 9H0124, 9H0391, 9H0736, 9H0380, 9H0400, 9H0434, 9H0590). Total small molecule extracts seedling leaves were...
Ruthenium polypyridyl complexes inhibit cancer growth by targeting TrxR and promote the intracellular ROS generation, ultimately triggering mitochondria-mediated cell apoptosis.
Thioredoxin system plays an important role in regulation of intracellular redox balance and various signaling pathways. reductase (TrxR) is overexpressed many cancer cells has been identified as a potential target anticancer drugs. Auranofin (AF) potent TrxR inhibitor with novel vitro vivo activities. Selenocystine (SeC) nutritionally available selenoamino acid selective effects through induction apoptosis. In the present study, we demonstrated synergistic underlying molecular mechanisms SeC...
Understanding molecular mechanisms underlying plant salinity tolerance provides valuable knowledgebase for effective crop improvement through genetic engineering. Current proteomic technologies, which support reliable and high-throughput analyses, have been broadly used exploring sophisticated networks in plants. In the current study, we compared phosphoproteomic changes roots of different soybean seedlings a salt-tolerant cultivar (Wenfeng07) salt-sensitive (Union85140) induced by salt...
The phylum Cnidaria represents a close outgroup to Bilateria and includes familiar animals including sea anemones, corals, hydroids, jellyfish. Here we report genome sequencing assembly for true jellyfish Sanderia malayensis Rhopilema esculentum. homeobox gene clusters are characterised by interdigitation of Hox, NK, Hox-like genes revealing an alternate pathway ANTP class dispersal intact three ParaHox cluster. mitochondrial genomes linear but, unlike in Hydra, do not detect nuclear copies,...
Long non-coding RNAs (lncRNAs) are defined as non-protein-coding transcripts that at least 200 nucleotides long. They known to play pivotal roles in regulating gene expression, especially during stress responses plants. We used a large collection of in-house transcriptome data from various soybean (Glycine max and Glycine soja) tissues treated under different conditions perform comprehensive identification lncRNAs. also retrieved publicly available were sufficient quality sequencing depth...
Abstract Whole genome duplication (WGD) has occurred in relatively few sexually reproducing invertebrates. Consequently, the WGD that common ancestor of horseshoe crabs ~135 million years ago provides a rare opportunity to decipher evolutionary consequences duplicated invertebrate genome. Here, we present high-quality assembly for mangrove crab Carcinoscorpius rotundicauda (1.7 Gb, N50 = 90.2 Mb, with 89.8% sequences anchored 16 pseudomolecules, 2 n 32), and resequenced tri-spine Tachypleus...
Clinical management of shrimp allergy is hampered by the lack accurate tests. Molecular diagnosis has been shown to more accurately reflect clinical reactivity but full spectrum allergens and their relevance are yet be established. We therefore sought comprehend allergen repertoire shrimp, investigate compare sensitization pattern diagnostic value in allergic subjects two distinct populations.Sera were collected from 85 with challenge-proven or doctor-diagnosed Hong Kong Thailand. The...
Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a new coronavirus strain, SARS-CoV. Specific proteomic patterns might be present in serum response to the infection and could useful for early detection of disease.Using surface-enhanced laser desorption/ionization (SELDI) ProteinChip technology, we profiled compared proteins 39 patients with early-stage SARS non-SARS who were suspected cases during outbreak period. Proteomic associated identified...
Lysine acetylation is a reversible post-translational modification (PTM) which has been linked to many biological and pathological implications. Hence, localization of lysine essential for deciphering the mechanism such Whereas acetylated lysines in human proteins have localized through experimental approaches wet lab, it still fails reach completion. In present study, we proposed novel feature extraction approach, bi-relative adapted binomial score Bayes (BRABSB), combined with support...
Abstract Human cyotsolic malate dehydrogenase (MDH1) is important in transporting NADH equivalents across the mitochondrial membrane, controlling tricarboxylic acid (TCA) cycle pool size and providing contractile function. Cellular localization studies indicate that MDH1 mRNA expression has a strong tissue‐specific distribution, being expressed primarily cardiac skeletal muscle brain, at intermediate levels spleen, kidney, intestine, liver, testes low lung bone marrow. The observed...
Abstract BACKGROUND. Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in world with a very poor prognosis. The majority ESCC patients present advanced metastatic disease upon diagnosis. Therefore, it important to understand molecular mechanism tumor invasion process and find new biomarkers for early diagnosis prognostic evaluation. METHODS. Differentially expressed proteins among different stages primary ESCCs their matched surrounding normal tissues were...
The Myriapoda, composed of millipedes and centipedes, is a fascinating but poorly understood branch life, including species with highly unusual body plan range unique adaptations to their environment. Here, we sequenced assembled 2 chromosomal-level genomes the Helicorthomorpha holstii (assembly size = 182 Mb; shortest scaffold/contig length needed cover 50% genome [N50] 18.11 Mb mainly on 8 pseudomolecules) Trigoniulus corallinus 449 Mb, N50 26.78 17 pseudomolecules). Unique genomic...