A5039983973- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Growth Hormone and Insulin-like Growth Factors
- Asthma and respiratory diseases
- Central Venous Catheters and Hemodialysis
- Metabolism, Diabetes, and Cancer
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer, Hypoxia, and Metabolism
- Vascular Procedures and Complications
- Ubiquitin and proteasome pathways
- Transgenic Plants and Applications
- Insect and Pesticide Research
- TGF-β signaling in diseases
- Phagocytosis and Immune Regulation
- Pancreatic function and diabetes
- Lipid metabolism and disorders
- Bone Metabolism and Diseases
- Monoclonal and Polyclonal Antibodies Research
- Identification and Quantification in Food
- Blood Coagulation and Thrombosis Mechanisms
- Nitric Oxide and Endothelin Effects
- Protein Hydrolysis and Bioactive Peptides
- Atherosclerosis and Cardiovascular Diseases
University of North Carolina at Chapel Hill
2011-2025
Chinese University of Hong Kong
2015-2025
Prince of Wales Hospital
2018-2025
University of North Carolina System
2022
Shatin Hospital
2017
University of North Carolina Health Care
2014
Indiana University School of Medicine
2013
Insulin-like growth factor I (IGF-I) is a mitogen for vascular smooth muscle cells (VSMC) and has been implicated in the development progression of atherosclerosis. IGF binding proteins (IGFBPs) modify IGF-I actions independently binding, but receptor-based mechanism by which they function not elucidated. We investigated role IGFBP-2 receptor protein tyrosine phosphatase β (RPTPβ) regulating signaling cellular proliferation. bound RPTPβ, led to its dimerization inactivation. This enhanced...
Summary Background Shellfish allergy is one of the most common food hypersensitivities worldwide but allergen‐specific immunotherapy for shellfish not yet available. We believe that T cell peptide‐based holds potential modulating allergic responses without IgE cross‐linking. Objective sought to identify immunodominant epitopes tropomyosin, major shrimp allergen Metapenaeus ensis (Met e 1), and evaluate their therapeutic effects in a Balb/c mouse model Met 1 hypersensitivity. Methods were...
Clinical management of shrimp allergy is hampered by the lack accurate tests. Molecular diagnosis has been shown to more accurately reflect clinical reactivity but full spectrum allergens and their relevance are yet be established. We therefore sought comprehend allergen repertoire shrimp, investigate compare sensitization pattern diagnostic value in allergic subjects two distinct populations.Sera were collected from 85 with challenge-proven or doctor-diagnosed Hong Kong Thailand. The...
Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin (Met e 1) has long been identified the major shellfish allergen, no immunotherapy is currently available. In this study, we aim at identifying Met 1 IgE epitopes construction hypoallergens and to determine inhibitory capacity hypoallergens. were defined by three online computational models, ELISA dot-blot using sera from allergy patients....
ABSTRACT Insulin-like growth factor binding protein 2 (IGFBP-2) is important for acquisition of normal bone mass in mice; however, the mechanism by which IGFBP-2 functions not defined. These studies investigated role stimulating osteoblast differentiation. MC-3T3 preosteoblasts expressed IGFBP-2, and knockdown resulted a substantial delay differentiation, reduced osteocalcin expression Alizarin red staining. findings were replicated primary calvarial osteoblasts obtained from IGFBP-2−/−...
IGF-I–stimulated sarcoma viral oncogene (Src) activation during hyperglycemia is required for propagating downstream signaling. The aim of the current study was to determine mechanism by which enhances Src and role NADPH oxidase 4 (Nox4) protein kinase C ζ (PKCζ) in mediating this response vascular smooth muscle cells (VSMCs). Nox4 expression analyzed VSMCs exposed hyperglycemia. Nox4-derived reactive oxygen species (ROS) investigated via knockdown Nox4. Different isoforms PKC were screened...
Arteriovenous fistulae (AVF) are the preferred mode of dialysis vascular access but have a maturation failure rate over 50%. Venous segment stenosis is one main reasons. However, reasons for prevalence in venous segment, as opposed to arterial remain unknown. We hypothesize that differences protein expression profiles and activation key signaling pathways smooth muscle cells (VSMCs) these two segments may contribute this difference. In study, porcine VSMCs (ApSMCs) (VpSMCs) were isolated...
Abstract In this study, we investigated the hemodynamic characteristics of arteriovenous fistulae (AVF) in murine models using micro-CT based computational fluid dynamics (CFD). By combining high-resolution imaging with ultrasound flow measurements, our methodology offers a cost-effective and efficient alternative to traditional MRI-based approaches. CFD simulations performed at 7 21 days post-surgery revealed significant temporal changes both geometry hemodynamics. Geometric analysis showed...
ABSTRACT Background IgE‐mediated fish allergy has long been considered an umbrella term due to the high cross‐reactivity of parvalbumin, major allergen. Yet, clinical tolerance certain highlights allergenicity differences. In this study, we sought construct a ladder and identify parvalbumin epitopes improve diagnosis allergy. Methods Reported history serum‐specific IgE (sIgE) responses 200 Chinese subjects with suspected were collected analyzed, while relative content in different was...
Abstract Global deletion of the Igfbp2 gene results in suppression bone turnover. To investigate role insulin-like growth factor-binding protein-2 (IGFBP-2) regulating osteoclast differentiation, we cultured Igfbp2−/− marrow cells and found a reduction number osteoclasts impaired resorption. Addition full-length IGFBP-2 restored fusion, determine molecular domains that were required for this effect to be manifest, transfected with constructs which heparin-binding (HBD) or IGF-binding...
Abstract Integrin-associated protein (IAP/CD47) has been implicated in macrophage-macrophage fusion. To understand the actions of CD47 on skeletal remodeling, we compared Cd47−/− mice with Cd47+/+ controls. weighed less and had decreased areal bone mineral density femurs were shorter length thinner cortices exhibited lower trabecular volume owing to number thickness. Histomorphometry revealed reduced bone-formation apposition rates, accompanied by osteoblast numbers. No differences...
IGF-binding protein (IGFBP)-2 overexpression confers resistance to high-fat feeding and inhibits the differentiation of preadipocytes in vitro. However, whether administration IGFBP-2 can regulate adipogenesis vivo domains that mediate this response have not been defined. contains 2 heparin-binding (HBD), which are localized linker region (HBD1) C-terminal (HBD2) IGFBP-2. To determine relative importance these domains, we used synthetic peptides as well mutagenesis. Both HBD1 HBD2 inhibited...