A5023578012- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- SARS-CoV-2 detection and testing
- Contact Dermatitis and Allergies
- Long-Term Effects of COVID-19
- Influenza Virus Research Studies
- Abdominal Trauma and Injuries
- Respiratory viral infections research
- COVID-19 and Mental Health
- Vaccine Coverage and Hesitancy
- Cardiac Arrest and Resuscitation
- Viral gastroenteritis research and epidemiology
- Medical Imaging and Pathology Studies
- Vascular Procedures and Complications
- Insect and Pesticide Research
- COVID-19 epidemiological studies
- Case Reports on Hematomas
- Renal and Vascular Pathologies
- Mind wandering and attention
- Bacterial Infections and Vaccines
- Trauma and Emergency Care Studies
- Innovative Teaching and Learning Methods
- Educational Games and Gamification
Queen Elizabeth Hospital
2018-2025
Hospital Authority
2021-2022
Changhua Christian Hospital
2010-2021
Sunway University
2019
Abstract The SARS-CoV-2 pandemic poses the greatest global public health challenge in a century. Neutralizing antibody is correlate of protection and data on kinetics virus neutralizing responses are needed. We tested 293 sera from an observational cohort 195 reverse transcription polymerase chain reaction (RT-PCR) confirmed infections collected 0 to 209 days after onset symptoms. Of 115 ≥61 illness using plaque reduction neutralization (PRNT) assays, 99.1% remained seropositive for both 90%...
SARS-CoV-2 infection of children leads to a mild illness and the immunological differences with adults are unclear. Here, we report specific T cell responses in infected find that acute memory CD4+ structural proteins increase age, whereas CD8+ time post-infection. Infected have lower ORF1ab when compared adults, comparable polyfunctionality reduced effector memory. Compared levels antibodies β-coronaviruses, indicating differing baseline immunity. Total follicular helper increased, whilst...
The duration of immunity in SARS-CoV-2 infected people remains unclear. Neutralizing antibody responses are the best available correlate protection against re-infection. Recent studies estimated that 50% from re-infection was 20% mean convalescent neutralizing titre.We collected sera a cohort 124 individuals with RT-PCR confirmed infections Prince Wales Hospital, Princess Margaret Queen Elizabeth Hospital and Mary Hospitals Authority Hong Kong, for periods up to 386 days after symptom onset...
<h3>Importance</h3> Schools were closed intermittently across Hong Kong to control the COVID-19 outbreak, which led significant physical and psychosocial problems among children youths. <h3>Objective</h3> To compare clinical characteristics sources of infection youths with during 3 waves outbreaks in 2020. <h3>Design, Setting, Participants</h3> This cross-sectional study involved aged 18 years or younger from January 23 through December 2, Data analyzed 2020 2021. <h3>Main Outcomes...
Abstract The antibody response magnitude and kinetics may impact clinical severity, serological diagnosis long-term protection of COVID-19, which play a role in why children experience lower morbidity. We therefore tested samples from 122 Hong Kong with symptomatic ( n = 78) asymptomatic 44) SARS-CoV-2 infections up to 200 days post infection, relative 71 infected adults (symptomatic 61, 10), negative controls 48). assessed serum IgG antibodies 14-wide antigen panel structural accessory...
Clinical management of shrimp allergy is hampered by the lack accurate tests. Molecular diagnosis has been shown to more accurately reflect clinical reactivity but full spectrum allergens and their relevance are yet be established. We therefore sought comprehend allergen repertoire shrimp, investigate compare sensitization pattern diagnostic value in allergic subjects two distinct populations.Sera were collected from 85 with challenge-proven or doctor-diagnosed Hong Kong Thailand. The...
ABSTRACT Background IgE‐mediated fish allergy has long been considered an umbrella term due to the high cross‐reactivity of parvalbumin, major allergen. Yet, clinical tolerance certain highlights allergenicity differences. In this study, we sought construct a ladder and identify parvalbumin epitopes improve diagnosis allergy. Methods Reported history serum‐specific IgE (sIgE) responses 200 Chinese subjects with suspected were collected analyzed, while relative content in different was...
Abstract Background Children are less clinically affected by SARS-CoV-2 infection than adults with the majority of cases being mild or asymptomatic and differences in outcomes poorly understood. The kinetics, magnitude landscape antibody response may impact clinical severity serological diagnosis COVID-19. Thus, a comprehensive investigation children is needed. Methods We tested 254 plasma from 122 symptomatic infections Hong Kong up to 206 days post symptom onset, including 146 longitudinal...
Pediatric COVID-19 studies exploring the relationships between NPS and saliva viral loads, clinical immunological profiles are lacking.Demographics, profiles, nasopharyngeal swab (NPS), samples collected on admission, hospital length of stay (LOS) were assessed in children below 18 years with COVID-19.91 patients included March August 20 20. loads correlated (r = 0.315, p 0.01). Symptomatic had significantly higher than asymptomatic patients. Serial load measurements showed that log10...
The current diagnostics of fish allergy lack sufficient accuracy such that more reliable tests as component-resolved diagnosis (CRD) are urgently needed. This study aimed at identifying allergens salmon and grass carp evaluating the sensitization pattern in allergic subjects from two distinct populations Asia. One hundred three were recruited Hong Kong (67 subjects) Japan (46 subjects). Western blot mass spectrometry used to identify carp. Fish purified tested against 96 sera on ELISA...
Abstract SARS-CoV-2 infection of children leads to a mild illness and the immunological differences with adults remains unclear. We quantified specific T cell responses in (<13 years age) RT-PCR confirmed asymptomatic symptomatic for long-term memory, phenotype polyfunctional cytokines. Acute memory CD4 + structural proteins significantly increased age, whilst CD8 time post infection. Infected had lower ORF1ab compared infected adults. SARS-CoV-2-specific were comparable magnitude...
Real-world Sensitization and Tolerance Pattern to Seafood inFish-Allergic Individuals Fish-allergic patients N=249By history sensitization (70%) By oral food challenge (30%)Selectively tolerant ≥ one fish, N=100 Shellfish co-sensitization, N=130 IgE levels fish extracts among 249 fish-allergic individuals Cod Halibut Salmon Tuna Grouper Herring Tilapia Catfish Grass carp P a r v l b u m i n T o e c An gradient clinical tolerance pattern corresponded the of parvalbumin in fish0% 10% 20% 30%...
Fish allergy is a significant health concern, with diagnosis and management complicated by diverse fish species allergens. We conducted comprehensive RNA-seq analysis of eight to identify allergen profiles, integrating ImmunoCAP sIgE data explore associations expression diagnostic performance. Over 30 putative allergens were identified, varying sequence similarities levels, roughly classifying into two groups based on parvalbumin (PV) expression. Higher in correlated stronger relationships...
Hong Kong has a high burden of influenza hospitalisation. This study estimated vaccine effectiveness in hospitalised children aged 6 months to below years using data potentially obtainable from routine surveillance sources.This 'test-negative' case-control was conducted over two summer and one winter seasons five public hospitals during 2015 2016. Patients admitted for febrile and/or respiratory-associated illnesses who met inclusion criteria were invited participate. Case-patients...
Chan, Wai-Hung MD; Huang, Hung Je Chen, Heng-Chung Lee, Po-Chang Chien-Pin Chang, Hung-Chi Yao-Li MD Author Information
The duration of immunity in SARS-CoV-2 infected people remains unclear. Neutralizing antibody responses are the best available correlate protection against re-infection. Recent studies have estimated that 50% from re-infection was 20% mean convalescent neutralizing titre. We used sera collected a cohort 125 individuals with RT-PCR confirmed infections up to 386 days after symptom onset. In subset 65 day 151 onset, all remained positive plaque reduction neutralization tests (PRNT50). Because...
Traumatic renal artery injury is an unusual result of blunt trauma. No meaningful recommendation has been established for optimal management on account a small number cases. Early diagnosis, however, important and advocated early at present includes nephrectomy, revascularization, endovascular stenting, thrombolytic agent administration careful observation renovascular injuries.A 60 year-old male suffered from traffic accident when he drove car hit telephone pole three days before. He was...
This exploratory study investigates perception towards a social anxiety therapy system developed during the course of teaching-learning systems analysis and design subsequently, repurposing human-computer interaction to improve proposed solution/system. The architectural is component-based. enables us extend suit different needs objectives. Preliminary user feedback obtained indicates positive negative perceptions. Based on feedback, original building blocks are retained but aspects...