A5108339784- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant and animal studies
- Biological and pharmacological studies of plants
- Plant Virus Research Studies
- Plant Reproductive Biology
- Membrane Separation Technologies
- Plant biochemistry and biosynthesis
- Wastewater Treatment and Nitrogen Removal
- Phytochemicals and Medicinal Plants
- Plant tissue culture and regeneration
- Anaerobic Digestion and Biogas Production
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant Parasitism and Resistance
- Methane Hydrates and Related Phenomena
- Water Treatment and Disinfection
Hangzhou Normal University
2016-2025
Air Force Medical University
2024
Tianjin Chengjian University
2020-2024
Chinese People's Liberation Army
2024
Nanyang Technological University
2024
Sichuan University
2015-2023
Army Medical University
2020-2022
UNSW Sydney
2022
Materials Science & Engineering
2022
Shaanxi Normal University
2019-2020
Tanshinones are health-promoting diterpenoids found in Salvia miltiorrhiza and have wide applications. Here, SmGGPPS (geranylgeranyl diphosphate synthase) SmDXSII (1-deoxy-d-xylulose-5-phosphate were introduced into hairy roots of S. miltiorrhiza. Overexpression produces higher levels tanshinone than control single-gene transformed lines; production the double-gene line GDII10 reached 12.93 mg/g dry weight, which is highest content that has been achieved through genetic engineering....
Abstract Auxin is involved in different aspects of plant growth and development by regulating the expression auxin‐responsive family genes. As one three major families, GH3 (Gretchen Hagen3) genes participate auxin homeostasis catalyzing conjugation bounding free indole‐3‐acetic acid (IAA) to amino acids. However, how function responses abiotic stresses various hormones maize largely unknown. Here, latest updated ( Zea mays L.) reference genome sequence was used characterize analyze ZmGH3...
Auxin response factors (ARFs) bind specifically to auxin elements (AuxREs) in the promoters of down-stream target genes and play roles plant responses diverse environmental factors. Using latest updated Medicago truncatula reference genome sequence, a comprehensive characterization analysis 24 MtARF (M. ARF) were performed. To uncover basic information functions during symbiosis, we analyzed expression patterns early phase Sinorhizobium meliloti infection. The systematic indicated that...
Abstract Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. . In this study, contents alkaloids various organs were measured compared. We took advantage transcriptomes from four to explore biological mechanisms organ-specific distribution active ingredients Based on Kyoto Encyclopedia Genes Genomes...
The auxin influx carriers resistant 1/like aux 1 (AUX/LAX), efflux pin-formed (PIN) (together with PIN-like proteins) and efflux/conditional P-glycoprotein (ABCB) are major protein families involved in polar transport. However, how they function responses to exogenous abiotic stresses maize is largely unknown. In this work, the latest updated (Zea mays L.) reference genome sequence was used characterize analyze ZmLAX, ZmPIN, ZmPILS ZmABCB family genes from maize. results showed that five...
Salinity causes osmotic stress to crops and limits their productivity. To understand the mechanism underlying soybean salt tolerance, proteomics approach was used identify phosphoproteins altered by NaCl treatment. Results revealed that 412 of 4698 quantitatively analyzed phosphopeptides were significantly up-regulated on treatment, including a phosphopeptide covering serine 59 in transcription factor GmMYB173. Our data showed GmMYB173 is one three MYB proteins differentially phosphorylated...
Microorganisms in anaerobic digestion (AD) are easily affected by ammonia, especially acetoclastic methanogens. Thus, ammonia-suppressed AD systems, acetate degradation is reported to be carried out mainly the cooperation of syntrophic oxidizers and hydrogenotrophic Previous studies have revealed ammonia inhibition on microbial flora performance, but effect mechanism metabolism remains poorly understood. In this study, we constructed a mesophilic chemostat fed with as sole carbon source,...
Taxus leaves provide the raw industrial materials for taxol, a natural antineoplastic drug widely used in treatment of various cancers. However, precise distribution, biosynthesis, and transcriptional regulation taxoids other active components remain unknown. Matrix-assisted laser desorption/ionization-mass spectrometry imaging analysis was to visualize secondary metabolites leaf sections mairei, confirming tissue-specific accumulation different metabolites. Single-cell sequencing produce...
Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little known about the precise distribution of taxoids and transcriptional regulation taxoid biosynthesis across stems. Here, we MALDI-IMS analysis to visualize mairei stems single-cell RNA sequencing generate expression profiles. A T. atlas created, providing spatial pattern cells. Cells were reordered using main developmental pseudotime trajectory provided temporal...
ABSTRACT The efficacy of Taxol, a natural anticancer drug, in the treatment various types cancers has been certified globally. Fungal elicitors have reported as an impressive strategy for enhancing Taxol biosynthesis. We investigated effect twig age on biosynthesis and communities endophytic fungi. A negative correlation between content complexity fungal community twigs was predicted. Endogenous taxoids, similar to balancing valves, might specific controlling microbiota assembly Taxus twigs....
BACKGROUND: Proton (H+) and aluminium (Al3+) toxicities are major factors limiting crop production on acid soils, while γ-aminobutyric (GABA) is a non-protein amino involved in various stress tolerances plants. In this study, to determine whether exogenous GABA functional alleviating oxidative induced by H+ Al3+ toxicities, the antioxidant defence response regulated was investigated barley (Hordeum vulgare L.). RESULTS: After 24 h treatments of seedlings under H+, combined stresses with...
In inducing photoperiodic conditions, plants produce a signal dubbed "florigen" in leaves. Florigen moves through the phloem to shoot apical meristem (SAM) where it induces flowering. Arabidopsis, FLOWERING LOCUS T (FT) protein acts as component of this phloem-mobile signal. However whether transportable FT mRNA also contributes systemic florigen signalling remains be elucidated. Using non-conventional approaches that exploit virus-induced RNA silencing and exclusion virus infection, we...
Understanding molecular mechanisms underlying plant salinity tolerance provides valuable knowledgebase for effective crop improvement through genetic engineering. Current proteomic technologies, which support reliable and high-throughput analyses, have been broadly used exploring sophisticated networks in plants. In the current study, we compared phosphoproteomic changes roots of different soybean seedlings a salt-tolerant cultivar (Wenfeng07) salt-sensitive (Union85140) induced by salt...
In plants, microRNAs (miRNAs) play essential roles in growth, development, yield, stress response and interactions with pathogens. However no miRNA has been experimentally documented to be functionally involved fruit ripening although many miRNAs have profiled fruits. Here we show that SlymiR157 SlymiR156 differentially modulate softening tomato (Solanum lycopersicum). is expressed developmentally regulated normal fruits those of the Colourless non-ripening (Cnr) epimutant. It regulates...
In non-climacteric fruits, the respiratory increase is absent and no phytohormone appearing to be critical for their ripening process. They must remain on parent plant enable full picked at or near fully ripe stage obtain best eating quality. However, huge losses often occur quick post-harvest senescence. To understanding complex mechanism of fruits senescence, we constructed two small RNA libraries one degradome from strawberry fruit stored 20°C 0 24 h. A total 88 known 1224 new candidate...
Several phytohormones have been demonstrated to be involved in iron (Fe) homeostasis. We took advantage of a salicylic acid (SA) biosynthesis defective mutant phytoalexin deficient 4 (pad4: T-DNA Salk_089936) explore the possible effects endogenous SA on morphological and physiological responses Fe deprivation. The analysis was carried out between Col-0 pad4 mutant. Under an Fe-deficiency treatment, showed more severe leaf chlorosis root growth inhibition compared with soluble concentrations...
Chrysanthemum morifolium, is a well-known flowering plant worldwide, and has high commercial, floricultural, medicinal value. In this study, simple-sequence repeat (SSR) markers were generated from EST datasets applied to assess the genetic diversity among 32 cultivars. A total of 218 in silico SSR loci identified 7300 C. morifolium ESTs retrieved GenBank. Of all loci, 61.47% them (134) hexa-nucleotide repeats, followed by tri-nucleotide repeats (17.89%), di-nucleotide (12.39%),...
Abstract Jasmonic acid (JA) is an important plant hormone involved in regulation of many aspects growth and development including secondary metabolism JASMONATE ZIM-DOMAIN (JAZ) proteins are key components JA signal processes. In this study, two new JAZ genes named SmJAZ3 SmJAZ9 were cloned from S. miltiorrhiza hairy roots characterized. Expression profiles under methyl jasmonate (MJ) treatment revealed that both MJ-responsive. Subcellular localization assay showed was located nucleus while...