Abstract ZEITLUPE (ZTL), a photoreceptor with E3 ubiquitin ligase activity, communicates end-of-day light conditions to the plant circadian clock. It still remains unclear how ZTL protein accumulates in but does not destabilize target proteins before dusk. Two deubiquitylating enzymes, UBIQUITIN-SPECIFIC PROTEASE 12 and 13 (UBP12 UBP13), which regulate clock period ubiquitylation manner opposite ZTL, associate complex. Here we demonstrate that interacting partner, GIGANTEA (GI), recruits...

Regulation of chemoreceptor gene expression in response to environmental or developmental cues provides a mechanism by which animals can alter their sensory responses. Here we demonstrate role for the daf-7 TGF-beta pathway regulation subset genes Caenorhabditis elegans. We describe novel this maintaining receptor adult and show that DAF-4 type II functions cell-autonomously modulate expression. also find alteration ASI chemosensory neurons signals, such as levels constitutively produced...

The Caenorhabditis elegans class A and B synthetic multivulva (synMuv) genes redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction. We identify a synMuv mutation in the promoter of lin-3 EGF gene, establishing that is key biological target development repressive activities pathways are integrated at level expression. Using FISH with single mRNA molecule resolution, we find expression tightly restricted only few tissues wild-type animals, including germline. In double...

Rhamnose is required in Arabidopsis thaliana for synthesizing pectic polysaccharides and glycosylating flavonols. RHAMNOSE BIOSYNTHESIS1 (RHM1) encodes a UDP-l-rhamnose synthase, rhm1 mutants exhibit many developmental defects, including short root hairs, hyponastic cotyledons, left-handed helically twisted petals roots. It has been proposed that the cotyledons observed are consequence of abnormal flavonol glycosylation, while hair defect flavonol-independent. We have recently shown helical...

Abstract Many mutations cause obvious abnormalities only when combined with other mutations. Such synthetic interactions can be the result of redundant gene functions. In Caenorhabditis elegans, multivulva (synMuv) genes have been grouped into multiple classes that redundantly inhibit vulval cell fates. Animals one or more same class undergo wild-type development, whereas animals any two a phenotype. By varying temperature and genetic background, we determined in most synMuv within single...

Plant cells and organs grow into a remarkable diversity of shapes, as directed by cell walls composed primarily polysaccharides such cellulose multiple structurally distinct pectins. The properties the wall that allow for precise control morphogenesis are from those individual polysaccharide components. For example, cellulose, primary determinant morphology, is chiral macromolecule can self-assemble in vitro larger-scale structures consistent chirality, yet most plant do not display...

The restricted expression of epidermal growth factor (EGF) family ligands is important for proper development and preventing cancerous in mammals. In Caenorhabditis elegans, the class A B synthetic multivulva (synMuv) genes redundantly repress lin-3 EGF to negatively regulate Ras-mediated vulval development. synMuv encode proteins homologous components NuRD Myb-MuvB/dREAM transcriptional repressor complexes, indicating that they likely silence through chromatin remodeling. two cloned thus...

Plants consist of many different cell types with specific shapes optimized for their particular functions. For example, most flowering plants have conically shaped epidermal cells on the upper surface petals that are important pollinator attraction. The control morphology in organs such as roots and leaves has been extensively studied, but much less is known about genes promote conical expansion petal cells. We developed a technique to rapidly assay cells, we employed this method an unbiased...

Abstract Rhamnose is required in Arabidopsis thaliana for synthesizing pectic polysaccharides and glycosylating flavonols. RHAMNOSE BIOSYNTHESIS1 (RHM1) encodes a UDP-L-rhamnose synthase, rhm1 mutants exhibit many developmental defects, including short root hairs, hyponastic cotyledons, left-handed helically twisted petals roots. It has been proposed that the cotyledons observed are consequence of abnormal flavonol glycosylation, while hair defect flavonol-independent. We have recently shown...

Abstract To remain synchronous with the environment, plants constantly survey daily light conditions using an array of photoreceptors and adjust their circadian rhythms accordingly. ZEITLUPE (ZTL), a blue photoreceptor E3 ubiquitin ligase activity, communicates end-of-day to clock. function properly, ZTL protein must accumulate but not destabilize target clock transcription factors before dusk, while in dark mediates degradation proteins. It is clear how can high levels its targets stable....