A5103045472- Sarcoma Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
- Cardiac tumors and thrombi
- Cancer Genomics and Diagnostics
- Cancer and Skin Lesions
- Lymphoma Diagnosis and Treatment
- Soft tissue tumor case studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Breast Lesions and Carcinomas
- Viral-associated cancers and disorders
- Pancreatic and Hepatic Oncology Research
- Genetic factors in colorectal cancer
- Single-cell and spatial transcriptomics
- Tumors and Oncological Cases
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Salivary Gland Tumors Diagnosis and Treatment
- Gene Regulatory Network Analysis
- Hedgehog Signaling Pathway Studies
- Polyomavirus and related diseases
- Galectins and Cancer Biology
- Geriatric Care and Nursing Homes
- Genetic and Kidney Cyst Diseases
- Nonmelanoma Skin Cancer Studies
- COVID-19 Clinical Research Studies
National Cancer Centre Singapore
2019-2025
Changi General Hospital
2023-2025
Duke-NUS Medical School
2024-2025
Singapore General Hospital
2022
Introduction Recent epidemiological data suggests a rising incidence of breast angiosarcoma (AS-B) in the Western population, with over two-thirds related to irradiation or chronic lymphedema. However, unlike head and neck (AS-HN), AS-B disease characteristics Asia remain unclear. Methods We examined clinical patterns patients (n = 176) seen an Asiantertiary cancer center from 1999 2021, specifically investigated molecular immune features comparison AS-HN. Data whole genome sequencing (WGS),...
Objective This study aimed to identify barriers and facilitators surrounding the implementation of TDOCS from Community Health Workers (CHW)'s perspective before implementation. Methods A descriptive qualitative was conducted through semistructured interviews with a purposive sampling CHWs partner nursing homes home care teams. French framework outlining asynchronous oral teleconsultation adoption used develop topic guide for this study. Then, Consolidated Framework Implementation research...
Abstract Angiosarcomas are rare malignant tumors of the endothelium, arising commonly from head and neck region (AS-HN) recently associated with ultraviolet (UV) exposure human herpesvirus-7 infection. We examined 81 cases angiosarcomas, including 47 AS-HN, integrating information whole genome sequencing, gene expression profiling spatial transcriptomics (10X Visium). In AS-HN cohort, we observed recurrent somatic mutations in CSMD3 (18%), LRP1B MUC16 POT1 (16%) TP53 (16%). UV-positive...
Abstract Idiopathic multicentric Castleman disease (iMCD) is a rare cytokine-driven disorder characterized by systemic inflammation, generalized lymphadenopathy, and organ dysfunction. Here, we present an unusual occurrence of iMCD in identical twins examined the immune milieu within affected lymphoid organs host circulation using multiomic high-dimensional profiling. Using spatial enhanced resolution omics sequencing (Stereo-seq) transcriptomic profiling, performed unsupervised spatially...
Fibroepithelial lesions (FELs) are a heterogeneous group of tumours comprising fibroadenomas (FAs) and phyllodes (PTs). Here we used 16-gene panel that was previously discovered to be implicated in pathogenesis progression, characterise large international cohort FELs via targeted sequencing. The study comprised 303 (38%) FAs 493 (62%) PTs which were contributed by the International Consortium. There 659 (83%) Asian 109 (14%) non-Asian FELs, while ethnicity rest unknown. Genetic aberrations...
Objectives Cholangiocarcinoma (CCA) is a heterogeneous malignancy with high mortality and dismal prognosis, an urgent clinical need for new therapies. Knowledge of the CCA epigenome largely limited to aberrant DNA methylation. Dysregulation enhancer activities has been identified affect carcinogenesis leveraged therapies but uninvestigated in CCA. Our aim identify potential therapeutic targets different subtypes through profiling. Design Integrative multiomics activity profiling diverse was...
Abstract Introduction: While traditionally informed by tumor tissue origin and histopathology, advancements in next-generation sequencing (NGS) technology have catalyzed tumor-agnostic approaches which prioritize molecular alterations for personalized treatment. Here, we demonstrate the clinical utility of molecularly guided precision medicine an Asian cohort, leveraging Asian-centric NGS panel tailored to address unique genetic landscape region. Methods: 1,167 formalin-fixed...
Peritoneal carcinomatosis (PC) present a ubiquitous clinical conundrum in all intra-abdominal malignancies. Via functional and transcriptomic experiments of ascites-treated PC cells, we identify STAT3 as key signaling pathway. Integrative analysis publicly available databases correlation with cohorts (n = 7,359) reveal putative clinically significant activating ligands signaling. We further validate 3-biomarker prognostic panel ascites independent covariates prospective study 149)....
Abstract Angiosarcomas are a rare subtype of soft-tissue sarcomas which exhibit aggressive clinical phenotypes with limited treatment options and poor outcomes. In this study, we investigated the relevance peripheral blood neutrophil-to-lymphocyte ratio (NLR) as marker systemic immune response, well its correlation intra-tumoral profiles in subgroup cases ( n = 35) using NanoString PanCancer IO360 panel multiplex immunohistochemistry. overall cohort 150), angiosarcomas head neck (AS-HN)...
Giant cells (GC) are a poorly understood subset of tumor that have been increasingly recognized as potential contributor to heterogeneity and treatment resistance. We aimed characterize the biological clinical significance GC in angiosarcoma, an aggressive rare cancer endothelial origin. Archival angiosarcoma samples were examined for presence compared with clinicopathological well NanoString gene expression data. cell lines MOLAS ISOHAS using conventional electron microscopy, single whole...
Abstract Recent epidemiological data suggests a rising incidence of breast angiosarcoma (AS-B) in the Western population, with over two-thirds related to irradiation or chronic lymphedema. However, unlike head and neck (AS-HN), AS-B disease characteristics Asia remains unclear. We examined clinical patterns patients (n = 176) seen an Asian tertiary cancer centre from 1999 2021, specifically investigated molecular immune features comparison AS-HN. Data whole genome sequencing (WGS) (AS-HN, n...
Abstract Malignant phyllodes tumors (PT) are rare aggressive fibroepithelial neoplasms with high metastatic potential and lack effective therapy. We established a patient-derived xenograft (PDX) cell line model (designated MPT-S1) of malignant PT which demonstrated clinical response to pazopanib. Whole exome sequencing identified somatic mutations in TP53 , RB1 MED12, KMT2D . Immunohistochemistry genomic profiles the tumor, PDX were concordant. In keeping observation, pazopanib reduced...
Abstract Background Extramammary Paget’s disease (EMPD) is a rare cancer that occurs within the epithelium of skin, arising predominantly in areas with high apocrine gland concentration such as vulva, scrotum, penis and perianal regions. Here, we aim to integrate clinicopathological data genomic analysis aggressive, rapidly-progressing de novo metastatic EMPD responding HER2-directed treatment combination other agents, attain more comprehensive understanding landscape. Methods...
Introduction: A well-validated diagnostic assay with curated biomarkers complements clinicopathological factors to facilitate early diagnosis and ensure timely treatment delivery. This study focuses on an Asian-centric cancer designed thoroughly validated against commercially available standard references a cohort of over 200 clinical specimens spanning 12 diverse types. Methods: The uses hybrid-capture probes capable profiling DNA aberrations from 572 cancer-related genes 91 RNA fusion...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected half a billion people, including vulnerable populations such as cancer patients. While increasing evidence supports the persistence of SARS-CoV-2 months after negative nasopharyngeal swab test, effects on long-term immune memory and treatment are unclear. In this report, we examined post-COVID-19 tissue-localized responses in hepatocellular carcinoma (HCC) patient colorectal (CRC) patient. Using spatial...
Abstract Introduction: Comprehensive molecular profiling with combined DNA-RNA next-generation sequencing (NGS) on tumor tissue is now increasingly being used as standard-of-care for sequence therapy selection to improve cancer survival. Combined NGS also considered central nervous system (CNS) classification. Despite the genetic heterogeneity and diversity of cancers, most commercial DNA/RNA assays are validated in Western populations, resulting knowledge gaps patient outcomes. We report...
Abstract Introduction: Brincidofovir (BCV) is a biologically-active acyclic nucleoside phosphonate and clinically-approved anti-viral agent. Recently, BCV has been demonstrated to display potent anti-proliferative immunogenic properties in NK/T cell lymphoma (NKTCL). However, its effect on other types remain unclear. Methods: We investigated an expanded cohort of B-cell cell-lines (diffuse large [DLBCL], n = 16; Burkitt lymphoma, 2; Hodgkin 1), comparison with NKTCL lines (n 11). Mouse...
Abstract Objectives: Cholangiocarcinoma (CCA) is a heterogeneous malignancy with high mortality and dismal prognosis, an urgent clinical need for new therapies. Knowledge of the CCA epigenome largely limited to aberrant DNA methylation. Dysregulation enhancer activities has been identified affect carcinogenesis leveraged therapies but uninvestigated in CCA. Our aim identify potential therapeutic targets different subtypes through profiling. Design: Integrative multi-omics activity profiling...