A5078565547- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- AI in cancer detection
- Single-cell and spatial transcriptomics
- Long-Term Effects of COVID-19
- Cell Image Analysis Techniques
- Lung Cancer Treatments and Mutations
- Pancreatic and Hepatic Oncology Research
- Animal Virus Infections Studies
- Glycosylation and Glycoproteins Research
- Renal cell carcinoma treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- COVID-19 and healthcare impacts
- Nanoplatforms for cancer theranostics
- Infection Control and Ventilation
- Intraperitoneal and Appendiceal Malignancies
- Phagocytosis and Immune Regulation
- Inflammasome and immune disorders
- Radiomics and Machine Learning in Medical Imaging
- Bioinformatics and Genomic Networks
Agency for Science, Technology and Research
2022-2025
Institute of Molecular and Cell Biology
2022-2025
Nanyang Technological University
2021
Abstract Gastric cancer (GC) is a major cause of global mortality with high levels heterogeneity. To explore geospatial interactions in tumor ecosystems, we integrated 2,138 spatial transcriptomic regions-of-interest (ROIs) 152,423 single-cell expression profiles across 226 GC samples from 121 patients. We observed pervasive expression-based intratumor heterogeneity, recapitulating progression through spatially localized and functionally ordered subgroups associated specific immune...
The World Health Organization has defined long COVID-19 (LC) as a condition that occurs in individuals with history of SARS-CoV-2 infection who exhibit persistent symptoms after its acute phase last for at least two months and cannot be explained by an alternative diagnosis. Since we had previously reported residual viral antigens tissues convalescent patients, aimed to assess the presence such COVID tissues. Here, established virus appendix, skin, breast 2 patients exhibited LC 163 426 days...
<div>Abstract<p>Gastric cancer is a major cause of global mortality. To explore geospatial interactions in gastric tumors, we integrated 2,138 spatial transcriptomic regions interest with 152,423 single-cell expression profiles across 226 samples from 121 patients. We observed pervasive expression-based intratumor heterogeneity, recapitulating tumor progression through spatially localized and functionally ordered subgroups associated specific immune microenvironments, checkpoint...
<p>(1) Supplementary Figure 1 shows morphologies of different ROI categories and their representative genes, pathways similarities with paired scRNA-seq data. (2) 2 validation findings from the discovery GeoMx DSP cohort in whole-section also TMA cohort. (3) 3 experiments validating that intratumor G1/G2 subregion expression is tumor-intrinsic using multiplexed IHC, Stereoseq spatial transcriptomics, vitro drug treatment profiling. (4) 4 individual immune checkpoints, chemokines,...
Introduction: Identification of immune cell types within bodily fluids, including blood samples, plays a critical role in early detection and disease monitoring public health initiatives. Although protein biomarkers present samples are useful for clinical assessment, the process sample preparation immunostaining is time-consuming costly. In this study, we an efficient rapid procedure affixing cells on glass slide downstream histological staining. Furthermore, using CD3+ T as paradigm, our...
Abstract Purpose: Peritoneal metastases (PM) in colorectal cancer (CRC) portend a poor prognosis. We sought to elucidate molecular features differentiating primary tumors (PTs) from PMs and actionable targets facilitating transcoelomic dissemination progression. Experimental Design: performed multi-omic profiling of 227 samples 136 patients, including 56 tumor (PT) 120 synchronous comprising 34 matched PT-PM pairs. Whole exome, bulk RNA-seq analysis was conducted identify underlying genomic...
Combination therapy with radioembolization (yttrium-90)-resin microspheres) followed by nivolumab has shown a promising response rate of 30.6% in Phase II trial (CA209-678) for advanced hepatocellular carcinoma (HCC); however, the mechanisms and relevant biomarkers remain unknown. By collecting both pretreatment on-treatment samples, we performed multimodal profiling tissue blood samples investigated molecular changes associated favorable responses 33 patients from trial. We found that...
Abstract Natural Killer (NK) cells have been shown to possess potent anti-tumor activity against multiple cancer types and consequently harnessed as a cellular immunotherapy. Furthermore, targeting of pancreatic stellate has received increasing interest in the treatment ductal adenocarcinoma (PDAC), with several clinical trials testing hypothesis that rendering activated cells/cancer associated fibroblasts (aPSC/CAF) into quiescent state (qPSC) can enhance anticancer treatment. Despite this...
Pancreatic ductal adenocarcinoma (PDAC) remains one of medicine's most urgent areas unmet need. With 5-year survival rates ∼11%, PDAC is set to become the second leading cause cancer related deaths by 2040 [1]. The complex tumour microenvironment (TME) in PDAC, responsible for poor prognosis, comprised extracellular matrix (ECM) proteins and multiple cell types; with pancreatic stellate cells (PSCs), which activated associated fibroblasts (CAFs), being regarded as key orchestrators TME. We...
Abstract The World Health Organization has defined long COVID-19 (LC) as a condition where patients exhibit persistent symptoms over time after its acute phase, which cannot be explained by alternative diagnosis. Since we have previously reported residual viral antigens in tissues of convalescent patients, now aim to assess the presence such post-convalescent tissues. Here, established virus within appendix and breast tissue 2 who exhibited LC symptoms, 175 462 days upon positive diagnosis,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected half a billion people, including vulnerable populations such as cancer patients. While increasing evidence supports the persistence of SARS-CoV-2 months after negative nasopharyngeal swab test, effects on long-term immune memory and treatment are unclear. In this report, we examined post-COVID-19 tissue-localized responses in hepatocellular carcinoma (HCC) patient colorectal (CRC) patient. Using spatial...
Abstract The World Health Organization has defined long COVID-19 (LC) as a condition where patients exhibit persistent symptoms over time after its acute phase, which cannot be explained by alternative diagnosis. Since we have previously reported residual viral antigens in tissues of convalescent patients, now aim to assess the presence such post-convalescent tissues. Here, established virus within appendix and breast tissue 2 who exhibited LC symptoms, 175 462 days upon positive diagnosis,...
Abstract Introduction The increased use of tissue-based multiplex immunohistochemistry (mIHC) in cancer research has significantly enhanced the generation spatial hyper-plex images, crucial for understanding complex tumor-immune microenvironments. This advancement led to a growing demand data analysis tools that enable quick and insightful evaluation. While existing software like Steinbock SPIAT offer foundational support, effectively managing high-dimensional voluminous cellular derive...
e14657 Background: Spatial multi-omics approaches, including spatial transcriptomics (ST) and proteomics (SP), that elucidate the complex tissue microenvironment (TME) are gaining traction in cancer research studies. However, combining these approaches onto one section remains challenging. ST methods visualize, locate quantify RNA targets, evaluating transcriptome of TME at subcellular level while SP stain visualize protein biomarkers cellular level. Here we show a pipeline combines with...
Inflammasome is linked to many inflammatory diseases, including COVID-19 and autoimmune liver diseases. While severe was reported exacerbate failure, we report a fatal acute-on-chronic failure (ACLF) in stable primary biliary cholangitis-autoimmune hepatitis overlap syndrome patient triggered by mild infection. Postmortem biopsy showed sparse SARS-CoV-2-infected macrophages with extensive ASC (apoptosis-associated speck-like protein containing CARD) speck-positive hepatocytes, correlating...
Recent advances in clinical and research pathology are fueled by new histomorphological molecular techniques, moving beyond traditional methods like Hematoxylin Eosin (H&E) immunohistochemical (IHC), which offer limited information have high observer variability. Advanced spatial omics provide detailed mapping but costly complex. Artificial intelligence (AI) has emerged as a promising solution to these challenges digitally predicting cellular biomarkers from H&E-stained images. This...
Abstract Triple-negative breast cancer (TNBC) is a highly aggressive subtype that generally associated with poor prognosis in affected patients. Unfortunately, recently approved immune checkpoint inhibitors are efficacious only subset of A surrogate marker for tumor-specific CD8 + T cells urgently required to clarify the mechanisms antitumor responses and help prognosticate TNBC Here, we conducted comparative interrogation evaluate potential CD39 as cells. We first used flow cytometry study...
<title>Abstract</title> Mutations in the PI3K pathway, particularly of <italic>PIK3CA</italic>, were reported to be intimately associated with triple negative breast cancer (TNBC) progression and development treatment resistance. We profiled <italic>PIK3CA</italic> other genes on 166 early-stage TNBC tumors from Singapore, for comparison publicly available cohorts. These transcriptionally using a Nanostring panel immune multiplex immunohistochemistry, then manually scored PD-L1-positivity...