A5078886672- Cancer-related Molecular Pathways
- Cervical Cancer and HPV Research
- Cancer Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Colorectal Cancer Treatments and Studies
- Molecular Biology Techniques and Applications
- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Reproductive tract infections research
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- FOXO transcription factor regulation
- Single-cell and spatial transcriptomics
- Circadian rhythm and melatonin
- Cancer Mechanisms and Therapy
- Gastrointestinal Tumor Research and Treatment
- Urinary and Genital Oncology Studies
Shandong Provincial Hospital
2025
Shandong First Medical University
2025
Agency for Science, Technology and Research
2012-2024
Institute of Molecular and Cell Biology
2022-2024
Shuguang Hospital
2024
Shanghai University of Traditional Chinese Medicine
2024
A*STAR Graduate Academy
2015-2022
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2020
Wenzhou Medical University
2020
Singapore Polytechnic
2019
The viral E2 gene product plays a crucial role in the human papillomavirus (HPV) vegetative cycle by regulating both transcription and replication of genome. is transcriptional repressor E6 E7 oncogenes for HPV types 16 18, which are involved cervical cancers. Using new polyclonal antibodies against HPV16 protein, we showed that expressed at various precursor stages carcinoma immunohistochemistry on paraffin-embedded clinical samples. was found to be highly nuclei cytoplasm cells forming...
The large number of mutations identified across all cancers represents an untapped reservoir targets that can be useful for therapeutic targeting if highly selective, mutation-specific reagents are available. We report here our attempt to generate such reagents: monoclonal antibodies against the most common R175H, R248Q, and R273H hotspot mutants tumor suppressor p53. These recognize their intended specific alterations without any cross-reactivity wild-type (WT) p53 or other mutants,...
The productive human papillomavirus (HPV) life cycle is tightly linked to the differentiation and cycling of keratinocytes. Deregulation these processes stimulation cell proliferation by action viral oncoproteins host factors underlies HPV-mediated carcinogenesis. Severe HPV infections characterize wart, hypogammaglobulinemia, infection, myelokathexis (WHIM) immunodeficiency syndrome, which caused gain-of-function mutations in CXCR4 receptor for CXCL12 chemokine, one CXCR41013. We...
The diagnosis of ovotesticular disorders sex development can only be confirmed when both testicular and ovarian tissues are present simultaneously in the same individual, regardless patient's karyotype. This report aims to discuss treatment a rare case complicated by dysgerminoma. patient, 24-year-old female, was admitted hospital due clitoral hypertrophy. Chromosomal analysis revealed chimeric karyotype 46, XX/46, XY. Biopsies ovaries indicated presence dysgerminoma right tissue....
Integration of the viral DNA in cellular genome has been suggested to be critical carcinogenic progression HPV-associated cervical neoplasia. This event can accompanied by disruption open reading frame (ORF) encoding E2 repressor, thus leading transcriptional up-regulation E6 and E7 oncogenes. At this stage, it is unclear whether ORF mandatory for progression. We measured RNA protein expression clinical samples various grades HPV16-associated neoplasia compared with status genome. extracted...
p53 protein, activated and stabilized by posttranslational modifications, performs its major functions inducing DNA repair, cell-cycle arrest, or apoptosis through transcriptional activation. Here, we determined the ability of protein via proteasome inhibition to perform similar as induced stresses such damage. Treating mice with inhibitor bortezomib in stem/progenitor cells intestine stomach, other proliferating tissues, intestinal tumors. Robust basal mRNA levels were observed same...
The tumour suppressor p53 is regulated primarily at the protein level. In normal tissues its levels are maintained a very low level by action of specific E3 ligases and ubiquitin proteosome pathway. mutant contributes to transformation, metastasis drug resistance. High can be found in tumours accumulation has previously been reported pathologically cells human skin. We show for first time that similarly elevated detected apparently knock-in mouse model. fact, small intestine, spontaneously...
Staple composition can influence target binding and bioactivity of peptides. We present strategies to modulate<italic>E</italic>/<italic>Z</italic>ratios access saturated analogues.
Objective: To Explore the relationship between HPV infection and bacterial vaginosis(BV), vulvovaginal candidiasis(VVC), trichomonal vaginitis(TV). Methods: Clinical data from 1 261 gynecologic outpatients who underwent vaginal microecology type detection during June 2015 to December were collected analyzed in First Affiliated Hospital of Xi'an Jiaotong University. Results: In patients, 328 cases infected with HPV, rate was 26.01%(328/1 261); infectious disease infections 219 cases(66.8%,...
Cervical cancer is the second most common cause of death from among women worldwide. Human papillomavirus (HPV) plays a central role in etiology cervical cancer. It important to describe prevalence HPV infection different types lesions and explore relation between viral load severity lesions.To age groups, we retrospectively investigated 6405 cases who were organized by their units take health-examination. They given Hybrid Capture II tests January 2005 December 2006. The correlation...
We have previously shown that proteasome inhibitor bortezomib stabilizes p53 in stem and progenitor cells within gastrointestinal tissues. Here, we characterize the effect of treatment on primary secondary lymphoid tissues mice. find significant fractions hematopoietic bone marrow, including common myeloid progenitors, granulocyte-monocyte dendritic cell progenitors. The stabilization is also observed multipotent progenitors cells, albeit at lower frequencies. In thymus, CD4-CD8- T cells....
// Yuezhen Xue 1, 5 , Shen Yon Toh 1 Pingping He Thimothy Lim 2 Diana 3 Chai Ling Pang 4 Jean-Pierre Abastado Françoise Thierry Institute of Medical Biology, A*STAR, Singapore Department Gynaecological Oncology, KK Women's and Children's Hospital, Pathology, National University Immunology Network, Current address: p53 Laboratory, Correspondence to: Xue, e-mail: yzxue@p53lab.a-star.edu.sg Keywords: HPV16-E2, DNA damage response, cell cycle, prophase, cervical intraepithelial neoplasia...
Overexpression of mutant p53 is a common finding in most cancers but testicular tumours accumulate wild-type (wtp53). In contrast to the accepted concept that homozygous mice do not normal cells, our study on mouse model Li-Fraumeni syndrome harbouring hot-spot p53R172H mutation described an elevated level non-cancerous tissues. Here we use detailed immunohistochemical analysis document expression testis. developing and adult testes, was expressed gonocytes, type A, Int, B spermatogonia as...
<h3>Background</h3> Tumor Immuno-MicroEnvironment (TIME) is characterized by a heterogeneous interplay of cellular and molecular components. For the TIME cancers caused virus infection, comparison immune cells close to far from viral crucial for understanding localized response developing targeted therapies, has not been possible investigate until advent spatial transcriptomic techniques. Here, we proposed methodology localize infection sites using SpaTial Enhanced REsolution...