Su Ting Tay
A5090510660- RNA modifications and cancer
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Renal cell carcinoma treatment
- Single-cell and spatial transcriptomics
- Genetic factors in colorectal cancer
- Chromatin Remodeling and Cancer
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Cancer Mechanisms and Therapy
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Treatments and Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gastrointestinal Tumor Research and Treatment
- Sarcoma Diagnosis and Treatment
- MicroRNA in disease regulation
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Nephrotoxicity and Medicinal Plants
- Immune cells in cancer
- RNA and protein synthesis mechanisms
Duke-NUS Medical School
2015-2025
Weatherford College
2023
National University of Singapore
2014-2016
National Cancer Centre Singapore
2003-2013
Gastric cancer heterogeneity represents a barrier to disease management. We generated comprehensive single-cell atlas of gastric (>200,000 cells) comprising 48 samples from 31 patients across clinical stages and histologic subtypes. identified 34 distinct cell-lineage states including novel rare cell populations. Many lineage exhibited cancer-associated expression profiles, individually contributing combined tumor-wide molecular collage. observed increased plasma proportions in diffuse-type...
Genome-wide mutational signatures of the group 1 carcinogen aristolochic acid are observed in urothelial cancers and liver from Asia.
Abstract Gastric cancer (GC) is a major cause of global mortality with high levels heterogeneity. To explore geospatial interactions in tumor ecosystems, we integrated 2,138 spatial transcriptomic regions-of-interest (ROIs) 152,423 single-cell expression profiles across 226 GC samples from 121 patients. We observed pervasive expression-based intratumor heterogeneity, recapitulating progression through spatially localized and functionally ordered subgroups associated specific immune...
Abstract Regulatory enhancer elements in solid tumours remain poorly characterized. Here we apply micro-scale chromatin profiling to survey the distal landscape of primary gastric adenocarcinoma (GC), a leading cause global cancer mortality. Integrating 110 epigenomic profiles from GCs, normal tissues and cell lines, highlight 36,973 predicted enhancers 3,759 super-enhancers respectively. Cell-line-defined can be subclassified by their somatic alteration status into gain, loss unaltered...
Abstract Protein-coding mutations in clear cell renal carcinoma (ccRCC) have been extensively characterized, frequently involving inactivation of the von Hippel–Lindau (VHL) tumor suppressor. Roles for noncoding cis-regulatory aberrations ccRCC tumorigenesis, however, remain unclear. Analyzing 10 primary tumor/normal pairs and 9 lines across 79 chromatin profiles, we observed pervasive enhancer malfunction ccRCC, with cognate enhancer-target genes associated tissue-specific aspects...
Abstract Background Deregulated gene expression is a hallmark of cancer; however, most studies to date have analyzed short-read RNA sequencing data with inherent limitations. Here, we combine PacBio long-read isoform (Iso-Seq) and Illumina paired-end comprehensively survey the transcriptome gastric cancer (GC), leading cause global mortality. Results We performed full-length analysis across 10 GC cell lines covering four major molecular subtypes (chromosomal unstable, Epstein-Barr positive,...
Intestinal metaplasia (IM) is a pre-malignant condition of the gastric mucosa associated with increased cancer (GC) risk. Analyzing 1,256 samples (1,152 IMs) across 692 subjects from prospective 10-year study, we identify 26 IM driver genes in diverse pathways including chromatin regulation (ARID1A) and intestinal homeostasis (SOX9). Single-cell spatial profiles highlight changes tissue ecology lineage heterogeneity, an stem-cell dominant cellular compartment linked to early malignancy....
Peritoneal metastasis (PM) in gastric cancer (GC) is associated with poor prognosis and significant morbidity. We sought to understand the genomic, transcriptomic, tumor microenvironment (TME) features that contribute peritoneal organotropism GC.
Objective Gastric cancer (GC) comprises multiple molecular subtypes. Recent studies have highlighted mesenchymal-subtype GC (Mes-GC) as a clinically aggressive subtype with few treatment options. Combining studies, we derived and applied consensus Mes-GC classifier to define the enhancer landscape revealing disease vulnerabilities. Design Transcriptomic profiles of ~1000 primary GCs cell lines were analysed derive classifier. Clinical genomic associations performed across >1200 patients...
Objective Gastric cancer (GC) is a leading cause of mortality, with ARID1A being the second most frequently mutated driver gene in GC. We sought to decipher -specific GC regulatory networks and examine therapeutic vulnerabilities arising from loss. Design Genomic profiling patients including Singapore cohort (>200 patients) was performed derive mutational signatures inactivation across molecular subtypes. Single-cell transcriptomic profiles -mutated GCs were analysed tumour...
Testicular germ cell tumors are the most common cancer diagnosed in young men, and seminomas type of these cancers. There have been no exome-wide examinations genes mutated or overall rates nonsilent somatic mutations tumors. The objective was to analyze determine which affected mutations. Eight matched normal samples were surgically obtained from eight patients. DNA extracted tissue exome sequenced on massively parallel Illumina sequencers. Single-nucleotide polymorphism chip-based copy...
<h3>Background</h3> GI stromal tumours (GISTs) are clinically heterogenous exhibiting varying degrees of disease aggressiveness in individual patients. <h3>Objectives</h3> We sought to identify genetic alterations associated with high-risk GIST, explore their molecular consequences, and test utility as prognostic markers. <h3>Designs</h3> Exome sequencing 18 GISTs was performed (9 patients high-risk/metastatic 5 low/intermediate-risk), corresponding 11 primary 7 metastatic tumours. Candidate...
Abstract Promoter elements play important roles in isoform and cell type–specific expression. We surveyed the epigenomic promoter landscape of gastric adenocarcinoma, analyzing 110 chromatin profiles (H3K4me3, H3K4me1, H3K27ac) primary cancers, cancer lines, nonmalignant tissues. identified nearly 2,000 alterations (somatic promoters), many deregulated various epithelial malignancies mapping frequently to alternative promoters within same gene, generating potential pro-oncogenic isoforms...
Colorectal cancer with metastases limited to the liver (liver-limited mCRC) is a distinct clinical subset characterized by possible cure surgery. We performed high-depth sequencing of over 750 cancer-associated genes and copy number profiling in matched primary, metastasis normal tissues characterize genomic progression 18 patients liver-limited mCRC. High depth Illumina use three different variant callers enable comprehensive accurate identification somatic variants down 2.5% allele...
218 Background: Peritoneal metastases (PM) in colorectal cancer (CRC) portend a poor prognosis. We sought to elucidate spatially resolved molecular features facilitating transcoelomic dissemination and progression. Methods: 50 PT, 116 PM 37 primary normal samples from 96 patients were retrieved profiled with digital spatial profiling (GeoMx DSP, Nanostring Technologies). Through unsupervised clustering, we characterized the microenvironment of tumor-stroma compartments studied roles stromal...
Abstract Background Within the tumor microenvironment (TME), association of B lymphocytes (B cells) with prognosis and therapy response in gastric cancer (GC) remains poorly characterized. We investigated predictive prognostic value cells, including their spatial organization within TME, one largest multi-cohort studies to date. Methods Using CD20 immunohistochemistry, we evaluated cell density resection specimens from 977 patients resectable GC across three cohorts, randomized phase III...
<div>Abstract<p>Gastric cancer is a major cause of global mortality. To explore geospatial interactions in gastric tumors, we integrated 2,138 spatial transcriptomic regions interest with 152,423 single-cell expression profiles across 226 samples from 121 patients. We observed pervasive expression-based intratumor heterogeneity, recapitulating tumor progression through spatially localized and functionally ordered subgroups associated specific immune microenvironments, checkpoint...
<p>(1) Supplementary Figure 1 shows morphologies of different ROI categories and their representative genes, pathways similarities with paired scRNA-seq data. (2) 2 validation findings from the discovery GeoMx DSP cohort in whole-section also TMA cohort. (3) 3 experiments validating that intratumor G1/G2 subregion expression is tumor-intrinsic using multiplexed IHC, Stereoseq spatial transcriptomics, vitro drug treatment profiling. (4) 4 individual immune checkpoints, chemokines,...