A5067176456- Intraperitoneal and Appendiceal Malignancies
- Ovarian cancer diagnosis and treatment
- Sarcoma Diagnosis and Treatment
- Appendicitis Diagnosis and Management
- Vascular Tumors and Angiosarcomas
- Ferroptosis and cancer prognosis
- Cardiac tumors and thrombi
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Molecular Biology Techniques and Applications
- Hernia repair and management
- Intestinal and Peritoneal Adhesions
- Cancer Genomics and Diagnostics
- Nanoparticle-Based Drug Delivery
- Bone Tumor Diagnosis and Treatments
- Inflammatory Biomarkers in Disease Prognosis
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Colorectal and Anal Carcinomas
- Hormonal Regulation and Hypertension
- Colorectal Cancer Treatments and Studies
- Fatty Acid Research and Health
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Nonmelanoma Skin Cancer Studies
National Cancer Centre Singapore
2019-2025
Singapore General Hospital
2020-2024
National University Hospital
2023
National University of Singapore
2014
Peritoneal metastasis (PM) in gastric cancer (GC) is associated with poor prognosis and significant morbidity. We sought to understand the genomic, transcriptomic, tumor microenvironment (TME) features that contribute peritoneal organotropism GC.
218 Background: Peritoneal metastases (PM) in colorectal cancer (CRC) portend a poor prognosis. We sought to elucidate spatially resolved molecular features facilitating transcoelomic dissemination and progression. Methods: 50 PT, 116 PM 37 primary normal samples from 96 patients were retrieved profiled with digital spatial profiling (GeoMx DSP, Nanostring Technologies). Through unsupervised clustering, we characterized the microenvironment of tumor-stroma compartments studied roles stromal...
Abstract Ovarian cancer is associated with poor prognosis. Platinum resistance contributes significantly to the high rate of tumour recurrence. We aimed identify a set molecular markers for predicting platinum sensitivity. A signature cisplatin sensitivity was generated using Genomics Drug Sensitivity in Cancer and The Genome Atlas databases. Four potential biomarkers (CYTH3, GALNT3, S100A14, ERI1) were identified optimized immunohistochemistry (IHC). Validation performed on cohort patients...
Peritoneal carcinomatosis (PC) present a ubiquitous clinical conundrum in all intra-abdominal malignancies. Via functional and transcriptomic experiments of ascites-treated PC cells, we identify STAT3 as key signaling pathway. Integrative analysis publicly available databases correlation with cohorts (n = 7,359) reveal putative clinically significant activating ligands signaling. We further validate 3-biomarker prognostic panel ascites independent covariates prospective study 149)....
Abstract Generation of large amounts genomic data is now feasible and cost-effective with improvements in next generation sequencing (NGS) technology. Ribonucleic acid (RNA-Seq) becoming the preferred method for comprehensively characterising global transcriptome activity. Unique to cytoreductive surgery (CRS), multiple spatially discrete tumour specimens could be systematically harvested analysis. To facilitate such downstream analyses, laser capture microdissection (LCM) utilized obtain...
The prognostic significance of inflammatory markers in solid cancers is well-established, albeit with considerable heterogeneity. This study sought to investigate the postoperative marker trend peritoneal carcinomatosis (PC), a focus on colorectal PC (CPC), and propose optimal surveillance periods cutoffs.Data were collected from prospectively maintained database patients treated at authors' institution April 2001 March 2019. platelet-lymphocyte ratio (PLR), neutrophil-lymphocyte (NLR),...
Abstract Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) is associated with significant perioperative morbidity mortality. We aim to generate validate a biomarker set predicting sensitivity Mitomycin-C refine selection of patients colorectal peritoneal metastasis (CPM) for this treatment. A signature was generated using data from Genomics Drug Sensitivity in Cancer The Genome Atlas. Validation performed on CPM who underwent CRS-HIPEC (n = 62)...
We have previously identified and validated a panel of molecular prognostic markers (ATP13A3, SSR3, ANO1) for Head Neck Squamous Cell Carcinoma (HNSCC). The aim this study was to investigate the consequence ATP13A3 dysregulation on signaling pathways, aid in formulating therapeutic strategy targeting ATP13A3-overexpressing HNSCC. Gene Set Enrichment Analysis (GSEA) performed HNSCC microarray expression data (Internal local dataset [n = 92], TCGA 232], EMBL 81]) identify pathways associated...
Abstract Background: The poor prognosis of peritoneal metastasis (PM) in colorectal cancer (CRC) is typically attributed to, and characterized by, resistance to systemic chemotherapy immunotherapy. evolutionarily conserved plasma-peritoneal barrier as well reduced tumor tissue vascularity contributes poorer responses systemically administered antineoplastic therapy. Beyond these, we sought understand transcriptomic variations important therapeutic targets implicated organotropism by...
Background Peritoneal sarcomatosis (PS) is a difficult entity to treat with limited options and guarded prognosis. We aimed determine if the addition of hyperthermic intraperitoneal chemotherapy (HIPEC) could offer superior local recurrence-free survival in patients retroperitoneal sarcoma at high risk developing PS as opposed extended resection alone. Methods This single arm, phase II intervention study where all recurrent localized considered were for enrolment (ClinicalTrials.gov...
Field cancerization is suggested to arise from imbalanced differentiation in individual basal progenitor cells leading clonal expansion of mutant that eventually replace the epithelium, although without evidence. We performed deep sequencing analyses characterize genomic and transcriptomic landscapes field change two patients with synchronous aerodigestive tract tumors. Our data support emergence numerous genetic alterations cancer-associated genes but refutes hypothesis founder mutation(s)...
Angiosarcomas of the breast (ASB) are rare, making up to less than 8% all angiosarcomas. The surgical management for this disease continues vary throughout centres worldwide due current limited evidence. We aim examine necessity axillary lymph node dissection in pathology through a retrospective study metastasis and recurrence patterns patients treated at our institution. A review prospectively-maintained database was performed. All adult with histologically confirmed diagnosis ASB seen...
Retroperitoneal sarcoma represents 15% of sarcomas. The mainstay treatment is surgery where a majority patients require multi-visceral resections that may significantly impact their quality life (QOL) following surgery. Studies in other cancers have shown QOL not be impacted after radical or extensive However, there are limited studies examining the specifically with retroperitoneal sarcoma. In this pilot study, we retrospectively evaluated sarcoma.32 out 90 who underwent surgical...
ABSTRACT Silver nanoparticles (AgNPs) are among the most commonly used nanomaterials, but thus far, little is known about ways to mitigate against potential toxic effects of exposure. In this study, we examined AgNPs on mitochondrial function and cellular ATP levels, whether these could be prevented by treatment with docosahexaenoic acid (DHA) l ‐carnitine (LC). Acute exposure for 1 h SH‐SY5Y cells resulted in decreased membrane potential, ADP indicating damage reduced production ATP....
Abstract Peritoneal metastases (PM) in gastric cancer (GC) portend a poor prognosis, yet our understanding of tumor microenvironmental (TME) characteristics associated with GCPM remain limited. Here, we analyzed intrinsic genomic alterations and transcriptomic programs predictive prospective cohort 248 patients, identifying CDH1 , PIGR ELF3 mutations as predictors. By inspecting the spatial dynamics TME, find that compartment infiltration pro-tumorigenic cell types such inflammatory...
Peritoneal metastasis (PM), the regional progression of intra-abdominal malignancies, is a common sequelae colorectal cancer (CRC). Immunotherapy slated to be effective in generating long-lasting anti-tumour response as it utilizes specificity and memory immune system. In tumour microenvironment, associated macrophages (TAMs) are posited create an anti-inflammatory pro-tumorigenic environment. this paper, we aimed identify immunomodulatory factors with PM (CPM). A publicly available single...