A5100696153- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- interferon and immune responses
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Immune cells in cancer
- Genetic Mapping and Diversity in Plants and Animals
- Cardiac Arrhythmias and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Atrial Fibrillation Management and Outcomes
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Immune Response and Inflammation
- Cardiac electrophysiology and arrhythmias
- Nanoplatforms for cancer theranostics
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- NF-κB Signaling Pathways
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Photoacoustic and Ultrasonic Imaging
Sun Yat-sen University Cancer Center
2016-2024
Sun Yat-sen University
2016-2024
Chongqing University
2023-2024
East China University of Science and Technology
2024
Peking University First Hospital
2018-2023
Peking University
2018-2023
State Key Laboratory of Oncology in South China
2021-2022
Ministry of Natural Resources
2022
Fujian Institute of Oceanography
2022
Dongguan University of Technology
2021
Significance Our study has identified a minimalist nanovaccine with C1 lipid nanoparticle (LNP) that effectively promotes mRNA delivery and antigen presentation self-adjuvant feature through activating TLR4 signaling. The LNP exhibited significant in vivo efficacy both tumor prevention therapeutic vaccine settings. Therefore, our work presents platform for developing cancer immunotherapy wide range of types.
Checkpoint blockade antibodies have been approved as immunotherapy for multiple types of cancer, but the response rate and efficacy are still limited. There few immunogenic cell death–inducing (ICD-inducing) drugs available that can kill cancer cells, enhance tumor immunogenicity, increase in vivo immune infiltration, thereby boost a to immunotherapy. So far, ICD markers identified immunostimulating characteristics dead whether presence such on cells translates into enhanced antitumor...
Abstract The immunocheckpoint protein PD-1/PD-L1 is considered a promising target for cancer immunotherapeutics. However, the objective response rate using antibodies that block interaction between PD-1 and PD-L1 was less than 40%, mechanism underlying regulation of expression poorly understood. In this study, we identified miRNA let-7 posttranscriptionally suppresses expression. LIN28, an RNA binding upregulated in most cells, inhibits biogenesis let-7, thus promoting Therefore, inhibition...
The importance of trained immunity in antitumor has been increasingly recognized, but the underlying metabolic regulation mechanisms remain incompletely understood. In this study, we find that squalene epoxidase (SQLE), a key enzyme cholesterol synthesis, is required for β-glucan-induced macrophages and ensuing activity. Unexpectedly, shunt pathway, not classical synthesis catalyzed by SQLE, induction. Specifically, 24(S),25-epoxycholesterol (24(S),25-EC), pathway metabolite, activates liver...
Abstract Cancer vaccines have shown promise as effective means of antitumor immunotherapy by inducing tumor antigen‐specific T cell immunity. In this study, a novel peptide‐based nanovaccine that boosts antigen presentation and elicits immunity is developed. The adjuvant characteristics an antimicrobial peptide‐derived core peptide, FK‐13, are investigated used it to generate fusion peptide named FK‐33 with epitopes. l ‐phenylalanine‐based poly(ester amide) (Phe‐PEA), 8p4, also identified...
Dynamic tracing of intracellular telomerase activity plays a crucial role in cancer cell recognition and correspondingly earlier diagnosis personalized precision therapy. However, due to the complexity required reaction system insufficient loading components into cells, achieving high-fidelity determination is still challenge. Herein, an Aptamer-Liposome mediated Telomerase activated poly-Molecular beacon Arborescent Nanoassembly(ALTMAN) approach was described for direct visualization...
Dysfunction of macroautophagy/autophagy has been postulated as a major cellular toxicological response to nanomaterials. It reported that excessive autophagy activation, induced by silica nanoparticles (SiNPs), contributes dysfunction, whereas little is known how SiNPs trigger activation. Here, we treated normal rat kidney (NRK) cells using 3 different sizes (16, 29, and 51 nm) observed 16-nm SiNPs, with final concentration 60 μg/mL, dramatically induce activation without reducing cell...
Prevalent copy number alteration is the most prominent genetic characteristic associated with ovarian cancer (OV) development, but its role in immune evasion has not been fully elucidated. In this study, we identified RAD21, a key component of cohesin complex, as frequently amplified oncogene that could modulate response OV. Through interrogating RAD21-regulated transcriptional program, found RAD21 directly interacts YAP/TEAD4 corepressors and recruits NuRD complex to suppress interferon...
Severe fever with thrombocytopenia syndrome (SFTS) is a novel emerging acute infectious disease caused by severe virus (SFTSV), characterized high and thrombocytopenia. It has been proved that traditional Chinese medicine (TCM) displayed definite therapeutic effects on viral hemorrhagic fever, indicating its potential to treat SFTS. In this study, SFTS-relative key targets were predicted via Gene ontology (GO) analysis Kyoto Encyclopedia of Genes Genomes (KEGG) enrichment analysis. Molecular...
Smad4, a key mediator of the transforming growth factor-β signaling, is mutated or deleted in 20% pancreatic ductal adenocarcinoma (PDAC) cancers and significantly affects cancer development. However, effect Smad4 loss on immunogenicity tumor immune microenvironment PDAC still unclear. Here, surprising function suppressing mouse identified. Although deletion cells enhances proliferation vitro, vivo Smad4-deficient inhibited immunocompetent C57BL/6 (B6) mice, but not immunodeficient mice CD8
Abstract The F-box proteins were originally identified as the key component of SKP1-Cullin1-F-box E3 ligase complexes that control stability their specific downstream substrates essential for cell growth and survival. However, involvement these in type I IFN (IFN-I) signaling during innate immunity has not been investigated. In this study we report protein FBXO17 negatively regulates IFN-I triggered by double-strand DNA, RNA, or viral infection. We found specifically interacts with...
Significance Reactive oxygen species (ROS) can promote tumorigenesis or kill cancer cells. How different cancer-associated genetic alterations regulate ROS balance and outcome is of great importance for the design rational treatments, many which affect metabolism sensing. Kras activation induces a defense system cell senescence, counteract its oncogenic activity. KRAS -activating mutations are accompanied by IKKα loss that result in elevated NOX2 but decreased expression NRF2 system. Thus,...
Induction of type I IFN (IFN-I) is essential for host antiviral immune responses. However, IFN-I also plays divergent roles in antibacterial immunity, persistent viral infections, autoimmune diseases, and tumorigenesis. regulatory factor 3 (IRF3) the master transcription that controls production via phosphorylation-dependent dimerization most cell types response to infections various innate stimuli by pathogen-associated molecular patterns (PAMPs). To monitor dynamic process IRF3 activation,...
CSGRqtl is a comparative genomic database that facilitates the cross-utilization of information among members Saccharinae clade grasses, and between other taxa. developed as specific data mining resource for crops, weeds, models, complementing supplementing another database, Gramene (Ware et al., 2002), includes variety mapping broad spectrum grass To facilitate comparisons, plant trait ontology defined by applied to categorize QTLs. Using Sorghum bicolor (L.) Moench (S.bicolor) genome...
Background Dendritic cells (DCs) play a critical role in antitumor immunity, but the therapeutic efficacy of DC-mediated cancer vaccine remains low, partly due to unsustainable DC function tumor antigen presentation. Thus, identifying drugs that could enhance DC-based immunity and uncovering underlying mechanism may provide new options for immunotherapy. Methods In vitro presentation assay was used DC-modulating drug screening. The T measured by flow cytometry, ELISA, or qPCR. B16, MC38,...
Abstract Hyperactive mevalonate (MVA) metabolic activity is often observed in cancer cells, and blockade of this pathway inhibits tumor cell lipid synthesis growth enhances immunogenicity. How MVA promotes antitumor immune responses, however, remains unclear. Here we show that inhibition the cells elicits type 1 classical dendritic (cDC1)–mediated recognition antigen cross-presentation for immunity. Mechanistically, disrupted prenylation small GTPase Rac1 induced actin filament exposure,...
The evolutionarily conserved F-box family of proteins are well known for their role as the key component SKP1-Cullin1-F-box (SCF) E3 ligase in controlling cell cycle, proliferation and death, carcinogenesis, cancer metastasis. However, thus far, there is only limited investigation on involvement antiviral immunity. In contrast to canonical function FBXO6 associated with SCF complex, we report, this study, that can also potently regulate activation IFN-I signaling during host response viral...
ABSTRACT Influenza virus RNA-dependent RNA polymerase consists of three viral protein subunits: PA, PB1, and PB2. Protein-protein interactions (PPIs) these subunits play pivotal roles in assembling the functional complex, which is essential for replication transcription influenza RNA. Here we developed a highly specific robust bimolecular luminescence complementation (BiLC) reporter system to facilitate investigation complex formation. Furthermore, by combining computational modeling BiLC...
Abstract Introduction Individual variability of rivaroxaban was observed in clinical application. This study aimed to identify genetic variants associated with the pharmacodynamics and bleeding risk rivaroxbaban patients nonvalvular atrial fibrillation (NVAF). Materials Methods From June 2017, July 2019, this enrolled 257 NVAF receiving rivaroxaban. Pharmacodynamics assessed by determining anti‐Factor Xa (anti‐FXa) level 3 h after administration as peak concentration. Whole‐exome sequencing...
A Gram-stain-positive, motile and rod-shaped bacterium, designated strain LZ2T, was isolated from a sample of orchard soil Laizhou city, Shandong province, PR China. On the basis 16S rRNA gene sequence analysis, LZ2T closely related to members genus Sporosarcina, sharing highest levels similarity with Sporosarcina pasteurii NCIMB 8841T (98.8 %), soli I80T (95.9 %). The value for DNA-DNA relatedness between 39.8±1.7 %. Growth occurred at 10-44 °C (optimum, 30-35 °C), pH 5.0-11.0 (optimum...
<p indent=0mm>Synthetic biology applies engineering principles for the deliberate design, engineering, and <italic>de novo</italic> creation of artificial biological systems with certain functions. Due to complexity living lack rational design principles, iterative trial-and-error experiments are often necessary, but dependence on human researchers limits throughput, efficiency, consistency such endeavor. To overcome these limitations, biofoundries developed as an integrated infrastructure...