A5030379162- Telomeres, Telomerase, and Senescence
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Identification and Quantification in Food
- Immunotherapy and Immune Responses
- Bacillus and Francisella bacterial research
- Receptor Mechanisms and Signaling
- Heat shock proteins research
- Influenza Virus Research Studies
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Alkaline Phosphatase Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune Response and Inflammation
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
- Pregnancy-related medical research
- Salmonella and Campylobacter epidemiology
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- RNA regulation and disease
Sun Yat-sen University
2014-2023
Eighth Affiliated Hospital of Sun Yat-sen University
2023
Sun Yat-sen University Cancer Center
2019
National University of Defense Technology
2016
National Chung Hsing University
2000
Centre National de la Recherche Scientifique
1994
Hôpital Cochin
1994
Abstract The immunocheckpoint protein PD-1/PD-L1 is considered a promising target for cancer immunotherapeutics. However, the objective response rate using antibodies that block interaction between PD-1 and PD-L1 was less than 40%, mechanism underlying regulation of expression poorly understood. In this study, we identified miRNA let-7 posttranscriptionally suppresses expression. LIN28, an RNA binding upregulated in most cells, inhibits biogenesis let-7, thus promoting Therefore, inhibition...
The RNA component of human telomerase (hTR) localizes to Cajal bodies, and it has been proposed that bodies play a role in the assembly holoenzyme trafficking. Here, was examined Human cells deficient coilin (i.e. coilin-knockout (KO) cells), which no are detected. In coilin-KO cells, normal level activity is detected interactions between core factors preserved, indicating occurs absence bodies. Moreover, dispersed hTR aggregates forms foci specifically during S G2 phase cells....
Abstract Telomerase consists of the catalytic protein TERT and RNA TERC . Mutations in are linked to human diseases, but underlying mechanisms poorly understood. Here we report that RNA-binding HuR associates with promotes assembly /TERT complex by facilitating C106 methylation. Dyskeratosis congenita (DC)-related U100A mutation impair association , thereby reducing Two other mutations aplastic anemia autosomal dominant DC, G107U, GC107/108AG, likewise disrupt methylation at C106....
Vascular calcification (VC) predicts the morbidity and mortality in cardiovascular diseases. smooth muscle cells (VSMCs) osteogenic transdifferentiation is crucial pathological basis for VC. To date, molecular pathogenesis still largely unclear. Notably, C5a-C5aR1 contributes to development of diseases, its closely related physiological bone mineralization which similar VSMCs transdifferentiation. However, role underlying mechanisms VC remain unexplored.
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications. Typically, sgRNA constitutively expressed relying on RNA polymerase III promoters. Polymerase II promoters initiate transcription in flexible manner, but sgRNAs generated by promoter lost their nuclease activity. To express tissue-specific fashion and endow CRISPR with more versatile function, novel system was established polycistron, where miRNAs (or shRNAs) alternately emerged co-expressed under...
Lethal-7 (let-7) microRNA (miRNA) serves a pivotal role in number of physiological processes and is associated with the occurrence development multiple disorders such as cancer. The present study aimed to use newly developed stem‑loop strategy for reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) distinguish let‑7 miRNA family members that differ by little single nucleotide. For miRNAs comprising 16 identical nucleotides at 5'‑end, different RT primers were designed used...
The majority of tumor cells overcome proliferative limit by expressing telomerase. Whether or not telomerase preferentially extends the shortest telomeres is still under debate. When human cancer are cultured at neutral pH, in telomere length-independent manner. However, microenvironment slightly acidic, and it yet known how this influences action. Here, we examine length homeostasis pHe 6.8. results indicate that short telomeres, such distribution narrows become nearly uniform size. After...
Premature telomere shortening is a known factor correlated to idiopathic pulmonary fibrosis (IPF) occurrence, which chronic, progressive, age-related disease with high mortality. The etiology of IPF still unknown. Here, we found that UBQLN1 plays key role in length maintenance and potentially relevant IPF. involves DNA replication by interacting RPA1 shuttling it off from the fork. deficiency retains at fork, hinders thus causes cell cycle arrest genome instability. Especially regions...
The miR-21 has been implicated in the process of neuroinflammation as well neuropathic pain.To explore relationship between plasma and local expression with disease severity lumbar disc herniation (LDH) patients sciatic pain.Ninety-two LDH pain 25 scoliosis painless controls were enrolled current study. Samples from nucleus pulposus (NP), annulus fibrosus (AF) soft tissues around nerve root (STANR) obtained. expressions detected quantitative reverse transcription polymerase chain reaction...
<div>Abstract<p>The immunocheckpoint protein PD-1/PD-L1 is considered a promising target for cancer immunotherapeutics. However, the objective response rate using antibodies that block interaction between PD-1 and PD-L1 was less than 40%, mechanism underlying regulation of expression poorly understood. In this study, we identified miRNA <i>let-7</i> posttranscriptionally suppresses expression. LIN28, an RNA binding upregulated in most cells, inhibits biogenesis...
<p>Figure S1.Inverse correlation between let-7e and PD-L1 expression. Figure S2.Let-7 suppresses S3.LIN28 mediates expression does not directly promote transcription. S4.C1632 in mouse cancer cells. S5.C1632 this inhibitory effect is rescued by LIN28 overexpression. S6.LIN28 inhibitor cell growth a variety of lines. S7.Depletion shRNA the proliferation TUBO S8.LIN28/let-7 axis regulates THP-1 macrophages.</p>
<p>Figure S1.Inverse correlation between let-7e and PD-L1 expression. Figure S2.Let-7 suppresses S3.LIN28 mediates expression does not directly promote transcription. S4.C1632 in mouse cancer cells. S5.C1632 this inhibitory effect is rescued by LIN28 overexpression. S6.LIN28 inhibitor cell growth a variety of lines. S7.Depletion shRNA the proliferation TUBO S8.LIN28/let-7 axis regulates THP-1 macrophages.</p>
<div>Abstract<p>The immunocheckpoint protein PD-1/PD-L1 is considered a promising target for cancer immunotherapeutics. However, the objective response rate using antibodies that block interaction between PD-1 and PD-L1 was less than 40%, mechanism underlying regulation of expression poorly understood. In this study, we identified miRNA <i>let-7</i> posttranscriptionally suppresses expression. LIN28, an RNA binding upregulated in most cells, inhibits biogenesis...
Zheng Tan (Fig. 1), Yong developed a method that enabled simultaneous and quantitative measurements of multiple kinetic parameters during nucleic acid duplex formation using an optical biosensor based on surface plasmon resonance.When applied to the study human telomere G-quadruplexes, it revealed dynamic structure telomeres helped expand application biosensors (Zhao et al., 2004).After completing his postdoctoral research in analytical chemistry at College Chemistry Molecular Sciences Wuhan...