A5083110315- Autophagy in Disease and Therapy
- Intracerebral and Subarachnoid Hemorrhage Research
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Parkinson's Disease Mechanisms and Treatments
- Fermentation and Sensory Analysis
- Biofuel production and bioconversion
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Livestock and Poultry Management
- Liver Disease Diagnosis and Treatment
- Synthetic Aperture Radar (SAR) Applications and Techniques
- Ocean Waves and Remote Sensing
- Optical Systems and Laser Technology
- Cancer-related Molecular Pathways
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Acute Ischemic Stroke Management
- Hops Chemistry and Applications
- Hepatitis B Virus Studies
- Neurosurgical Procedures and Complications
- Epigenetics and DNA Methylation
- Lipid metabolism and disorders
Tongde Hospital of Zhejiang Province
2019-2024
Huazhong University of Science and Technology
2019-2021
As an important post-translational modification (PTM), protein phosphorylation is involved in the regulation of almost all biological processes eukaryotes. Due to rapid progress mass spectrometry-based phosphoproteomics, a large number sites (p-sites) have been characterized but remain be curated. Here, we briefly summarized current progresses development data resources for collection, curation, integration and annotation p-sites eukaryotic proteins. Also, designed site database (EPSD),...
Dysfunction of macroautophagy/autophagy has been postulated as a major cellular toxicological response to nanomaterials. It reported that excessive autophagy activation, induced by silica nanoparticles (SiNPs), contributes dysfunction, whereas little is known how SiNPs trigger activation. Here, we treated normal rat kidney (NRK) cells using 3 different sizes (16, 29, and 51 nm) observed 16-nm SiNPs, with final concentration 60 μg/mL, dramatically induce activation without reducing cell...
Seeing is believing. The direct observation of GFP-Atg8 vacuolar delivery under confocal microscopy one the most useful end-point measurements for monitoring yeast macroautophagy/autophagy. However, manually labelling individual cells from large-scale sets images time-consuming and labor-intensive, which has greatly hampered its extensive use in functional screens. Herein, we conducted a time-course analysis nitrogen starvation-induced autophagy wild-type knockout mutants 35...
Intracerebral hemorrhage (ICH) accounts for approximately 15% of all stroke cases. Previous studies suggested that acupuncture may improve ICH-induced neurological deficits. Therefore, we investigated the effects on deficits in an animal model ICH.Adult male Sprague-Dawley rats were injected with autologous blood (50 μL) into right caudate nucleus. Additional underwent sham surgery as controls. ICH either received (GV20 through GB7 side lesion) or (1 cm to traditional point locations). Some...
In Saccharomyces cerevisiae, Atg9 is an important autophagy-related (Atg) protein, and interacts with hundreds of other proteins. How many Atg9-interacting proteins are involved in macroautophagy/autophagy unclear. Here, we conducted a multi-omic profiling Atg9-dependent molecular landscapes during nitrogen starvation-induced autophagy, identified 290 256 genes to be markedly regulated by ATG9 transcriptional translational levels, respectively. Unexpectedly, found most known Atg autophagy...
To observe the effect of penetrative needling from "Baihui" (GV20) to "Qubin" (GB7) on neurological function and expression autophagy related protein microtubule-associated protein, light chain 3 (LC3) in rats with hemorrhagic stroke, so as explore its mechanism underlying improvement stroke.A total 120 male SD were randomly divided into sham operation, model, non-acupoint, acupuncture medication (Rapamycin) groups which further two time-point subgroups (3, 7 days after modeling,...
To observe the effect of acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage (ICH), and to explore action mechanism promoting recovery neural function ICH.Forty SPF six-week-old male SD were randomly divided into a sham operation group, model non-acupoint group an 10 each group. The intervened autologous blood injection prepare ICH model, while only but not blood. About 3 hours after establishment 7), once every 12...