A5016147403- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Light effects on plants
- Plant Stress Responses and Tolerance
- Genomics and Chromatin Dynamics
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Plant responses to water stress
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Oral microbiology and periodontitis research
- Differential Equations and Boundary Problems
- Differential Equations and Numerical Methods
- Legume Nitrogen Fixing Symbiosis
- Intramuscular injections and effects
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Agriculture and Biological Studies
- Postharvest Quality and Shelf Life Management
Radboud University Nijmegen
2020-2024
Radboud Institute for Molecular Life Sciences
2022-2023
Radboud University Medical Center
2022-2023
Institute of Cytology and Genetics
2013-2022
Siberian Branch of the Russian Academy of Sciences
2013-2022
Novosibirsk State University
2007-2021
Russian Academy of Sciences
2006-2016
The phytohormone salicylic acid (SA) is well known for its induction of pathogenesis-related proteins and systemic acquired resistance; SA also has specific effects on plant growth development. Here we analyzed the effect Arabidopsis (Arabidopsis thaliana) root We show that exogenous treatment at low (below 50 µM) high (greater than concentrations affect meristem development in two different PR1-independent ways. Low-concentration promoted adventitious roots altered architecture apical...
Theauxin efflux carrier PIN1 is a key mediator of polar auxin transport in developing plant tissues. This why factors that are supposed to be involved distribution frequently tested the regulation expression. As result, diverse aspects expression dispersed across dozens papers entirely devoted other specific topics related pathway. Integration these puzzle pieces about revealed that, along with recurring pattern, some features varied from article article. To determine if this uncertainty...
Significance The plant hormone auxin controls many aspects of growth and development. It does so by changing the activity AUXIN RESPONSE FACTOR (ARF) proteins that recognize specific DNA elements in genes switch gene expression on or off. A major question biology is how these ARF bind unique sequences thus select which are regulated auxin. Here, authors systematically study architecture required for high-affinity binding proteins, auxin-dependent regulation Arabidopsis thaliana . This work...
Significance Plants show remarkable regenerative abilities, allowing them to recover from wounds and organ loss. This capacity is controlled in part by auxin, the most promiscuous plant hormone controlling organogenesis tissue patterning. We that stem cell death diverges auxin flow, much like rocks a stream, resulting an accumulation tissues surrounding wound. demonstrate within these tissues, wound-induced expression of plant-specific transcription factor ERF115 works synergistically with...
Abstract Genome-wide gene expression maps with a high spatial resolution have substantially accelerated plant molecular science. However, the number of characterized tissues and growth stages is still small due to limited accessibility most for protoplast isolation. Here, we provide profiles mature inflorescence stem Arabidopsis thaliana covering comprehensive set distinct tissues. By combining fluorescence-activated nucleus sorting laser-capture microdissection next-generation RNA...
Abstract Background In plant roots, auxin is critical for patterning and morphogenesis. It regulates cell elongation division, the development maintenance of root apical meristems, other processes. Arabidopsis , distribution along central axis has several maxima: in tip, basal meristem at shoot/root junction. The distal maximum tip maintains stem niche. Proximal maxima may trigger lateral or adventitious initiation. Results We propose a reflected flow mechanism formation meristem. based on...
Auxin responsive elements (AuxRE) were found in upstream regions of target genes for ARFs (Auxin response factors). While Chip-seq data most are still unavailable, prediction potential AuxRE is restricted by consensus models that detect too many false positive sites. Using sequence analysis experimentally proven AuxREs, we revealed both an extended nucleotide context pattern itself and three distinct types its coupling motifs (Y-patch, AuxRE-like, ABRE-like), which together with may form the...
Recognition of composite elements consisting two transcription factor binding sites gets behind the studies tissue-, stage- and condition-specific transcription. Genome-wide data on generated with ChIP-seq method facilitate an identification elements, but existing bioinformatics tools either require datasets for both partner factors, or omit motifs overlapping. Here we present universal Motifs Co-Occurrence Tool (MCOT) that retrieves maximum information about overrepresented from a single...
Root stem cell niche functioning requires the formation and maintenance of specific "auxin-rich domain" governed by directional auxin transport local production. Auxin maximum co-localizes with WOX5 expression domain in quiescent center that separates mitotically active proximal distal root meristems. Here we unravel interconnected processes happening under overexpression combining vivo experiments mathematical modeling. We showed WOX5-induced TAA1-mediated biosynthesis is cause, whereas...
The root apical meristem (RAM) is the plant stem cell niche which provides for formation and continuous development of root. Auxin main regulator RAM functioning, auxin maxima coincide with sites initiation maintenance. gradients are formed due to local biosynthesis polar transport. PIN family transporters plays a critical role in transport, two mechanisms maximum based on PIN-mediated transport have been proposed date: reverse fountain reflected flow mechanisms. combined here silico studies...
To date CYCB1;1 marker and cortex cell lengths have been conventionally used to determine the proliferation activity of Arabidopsis root meristem. By creating a 3D map mitosis distribution we showed that these markers overlooked stele endodermis save their potency divide longer than epidermis. Cessation divisions is not random process, so mitotic within shows diarch pattern. Mitotic all tissues peaked at same distance from quiescent center (QC); however, different stopped dividing distances,...
Abstract Auxin plays a pivotal role in virtually every aspect of plant morphogenesis. It simultaneously orchestrates diverse variety processes such as cell wall biogenesis, transition through the cycle, or metabolism wide range chemical substances. The coordination principles for complex orchestration are poorly understood at systems level. Here, we perform an RNA-seq experiment to study transcriptional response auxin treatment within gene groups different biological processes, molecular...
Development of plant vascular tissues involves tissue identity specification, growth, pattern formation and cell-type differentiation. Although later developmental steps are understood in some detail, it is still largely unknown how the initially specified. We used early
ABSTRACT The auxin signaling molecule regulates a range of plant growth and developmental processes. core transcriptional machinery responsible for auxin-mediated responses is conserved across all land plants. Genetic, physiological molecular exploration in bryophyte angiosperm model species have shown both qualitative quantitative differences responses. Given the highly divergent ontogeny dominant gametophyte (bryophytes) sporophyte (angiosperms) generations, however, it unclear whether...
Abstract The auxin signalling molecule regulates a range of plant growth and developmental processes. core transcriptional machinery responsible for auxin-mediated responses is conserved across all land plants. Genetic, physiological molecular exploration in bryophyte angiosperm model species have shown both qualitative quantitative differences responses. Given the highly divergent ontogeny dominant gametophyte (bryophytes) sporophyte (angiosperms) generations, however, it unclear whether...