A5029228108- Enzyme Structure and Function
- Aldose Reductase and Taurine
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Heme Oxygenase-1 and Carbon Monoxide
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Prenatal Substance Exposure Effects
- Protein Structure and Dynamics
- Research in Cotton Cultivation
- Antibiotic Resistance in Bacteria
- Plant Stress Responses and Tolerance
- Fungal Plant Pathogen Control
- Cancer, Hypoxia, and Metabolism
- Plant Gene Expression Analysis
- Advanced Glycation End Products research
- Enzyme Production and Characterization
- DNA Repair Mechanisms
- Retinoids in leukemia and cellular processes
- Eicosanoids and Hypertension Pharmacology
- Synthesis and Characterization of Pyrroles
- Clostridium difficile and Clostridium perfringens research
ALBA Synchrotron (Spain)
2016-2025
Universitat Autònoma de Barcelona
2014-2020
Roslin Cells (United Kingdom)
2017
Significance The plant hormone auxin controls many aspects of growth and development. It does so by changing the activity AUXIN RESPONSE FACTOR (ARF) proteins that recognize specific DNA elements in genes switch gene expression on or off. A major question biology is how these ARF bind unique sequences thus select which are regulated auxin. Here, authors systematically study architecture required for high-affinity binding proteins, auxin-dependent regulation Arabidopsis thaliana . This work...
The signaling molecule auxin coordinates many growth and development processes in plants, mainly through modulating gene expression. Transcriptional response is mediated by the family of factors (ARF). Monomers this recognize a DNA motif can homodimerize their DNA-binding domain (DBD), enabling cooperative binding to an inverted site. Most ARFs further contain C-terminal PB1 that capable homotypic interactions mediating with Aux/IAA repressors. Given dual role domain, ability both DBD...
The Auxin Response Factors (ARFs) family of transcription factors are the central mediators auxin-triggered transcriptional regulation. Functionally different classes extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part evolutionary trajectory to present auxin response functions has been reconstructed, it is unclear how emerged, early diversification led functionally proteins. Here, we use in silico vivo analyses revisit molecular events that origin...
Antibiotic resistance is a serious global problem. genes (ARG), which are widespread in environmental bacteria, can be transferred to pathogenic bacteria via horizontal gene transfer (HGT). Gut microbiomes especially apt for the emergence and dissemination of ARG. Conjugation HGT route that predominantly responsible spread Little known about conjugative elements Gram-positive including those phylum Firmicutes, abundantly present gut microbiomes. A critical step conjugation process...
Abstract The Auxin Response Factors (ARFs) family of transcription factors are the central mediators auxin-triggered transcriptional regulation. Functionally different classes extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part evolutionary trajectory to present auxin response functions has been reconstructed, it is unclear how emerged, early diversification led functionally proteins. Here, we use in silico vivo analyses revisit molecular events that...
Human aldo-keto reductase 1B15 (AKR1B15) is a newly discovered enzyme which shares 92% amino acid sequence identity with AKR1B10. While AKR1B10 well characterized high retinaldehyde activity, involved in the development of several cancer types, enzymatic activity and physiological role AKR1B15 are still poorly known. Here, purified recombinant has been subjected to substrate specificity characterization, kinetic analysis inhibitor screening, combined structural modeling. active towards...
Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced intense beam. Several effects, such as aggregation in protein solutions global site-specific crystals, reduce data quality or even introduce artefacts that can result a biologically misguiding structure. One strategy to these negative effects is inclusion of an additive buffer solution act free radical scavenger....
Abstract The human enzymes aldose reductase (AR) and AKR1B10 have been thoroughly explored in terms of their roles diabetes, inflammatory disorders, cancer. In this study we identified two new lead compounds, 2‐(3‐(4‐chloro‐3‐nitrobenzyl)‐2,4‐dioxo‐3,4‐dihydropyrimidin‐1(2 H )‐yl)acetic acid (JF0048, 3 ) 2‐(2,4‐dioxo‐3‐(2,3,4,5‐tetrabromo‐6‐methoxybenzyl)‐3,4‐dihydropyrimidin‐1(2 (JF0049, 4 ), which selectively target these enzymes. Although share the 3‐benzyluracil‐1‐acetic scaffold, they...
Abstract Human aldose reductase (hAR, AKR1B1) has been explored as drug target since the 1980s for its implication in diabetic complications. An activated form of hAR was found cells from patients, showing a reduced sensitivity to inhibitors clinical trials, which may prevent pharmacological use. Here we report conversion native by X-ray irradiation simulating oxidative stress conditions. Upon irradiation, enzyme activity increases moderately and potency several decay before global protein...
The principal route for dissemination of antibiotic resistance genes is conjugation by which a conjugative DNA element transferred from donor to recipient cell. Conjugative elements contain that are important their establishment in the new host, instance counteracting host defense mechanisms acting against incoming foreign DNA. Little known about these and how they regulated. Here, we deciphered regulation mechanism possible plasmid p576 Gram-positive bacterium Bacillus pumilus. Unlike ssDNA...
Transcriptional regulation usually requires the action of several proteins that either repress or activate a promotor an open reading frame. These can counteract each other, thus allowing tight transcription corresponding genes, where repression is often linked to DNA looping cross-linking. Here, tetramerization domain bacterial gene repressor Rco from Bacillus subtilis plasmid pLS20 (Rco ) has been identified and its structure shown share high similarity well known p53 family human tumor...
Auxin controls numerous growth processes in land plants through a gene expression system that modulates ARF transcription factor activity 1–3 . Gene duplications families encoding auxin response components have generated tremendous complexity most plants, and neofunctionalization enabled various unique outputs during development 2–4 However, it is unclear what fundamental biochemical principles underlie this complex system. By studying the minimal Marchantia polymorpha , we derive an...
Quorum sensing allows bacterial cells to communicate through the release of soluble signaling molecules into surrounding medium. It plays a pivotal role in controlling conjugation Gram-positive cells, process that has tremendous impact on health. Intracellular regulatory proteins RRNPP family are common targets these molecules. The gene regulators bind at their C-terminal domain (CTD), but have highly divergent functionalities N-terminal effector domains (NTD). This divergence is also...
Bacterial conjugation is an important route for horizontal gene transfer. The initial step in this process involves a macromolecular protein-DNA complex called the relaxosome, which plasmids consists of origin transfer (oriT) and several proteins that prepare relaxosome protein named relaxase introduces nick one strands oriT to initiate process. Additional can exist. Recently, encoded on Bacillus subtilis plasmid pLS20 were identified, including relaxase, RelpLS20, two auxiliary DNA-binding...
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number (SSX) experiments has grown exponentially, with over 40 reported so far. In this work, we report first SSX viscous jets conducted ALBA beamline BL13-XALOC. Small crystals (15–30 µm) five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended lipidic cubic phase (LCP) delivered to X-ray beam a high-viscosity injector...
Abstract Apical hook development is an ideal model for studying differential growth in plants, and controlled by complex hormonal crosstalk, with auxin ethylene being the major players. Here, we identified a bioactive small molecule that decelerates apical opening Arabidopsis thaliana . Our genetic studies suggest this enhances or maintains maximum found inner side requires certain signaling components to modulate opening. Using biochemical approaches, then revealed WD40 repeat scaffold...