A5027417160- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Berberine and alkaloids research
- Galectins and Cancer Biology
- Biochemical and Molecular Research
- NF-κB Signaling Pathways
- Oral and gingival health research
- Cancer-related Molecular Pathways
- Renal cell carcinoma treatment
- Natural product bioactivities and synthesis
- Carbohydrate Chemistry and Synthesis
- Neuroendocrine Tumor Research Advances
- Spectroscopy Techniques in Biomedical and Chemical Research
- Synthesis of Organic Compounds
- Metabolomics and Mass Spectrometry Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Ferroptosis and cancer prognosis
Khon Kaen University
2016-2025
Baylor College of Medicine
2018
Abstract Increased glucose utilization is a feature of cancer cells to support cell survival, proliferation, and metastasis. An association between diabetes mellitus progression was previously demonstrated in cancers including cholangiocarcinoma (CCA). This study aimed determine the effects high on protein O-GlcNAcylation metastatic potentials CCA cells. Two pairs each parental low highly sublines were cultured normal (5.6 mM) or (25 media. The migration invasion abilities determined...
Abstract Epidemiological studies have indicated diabetes mellitus (DM) as a risk of cholangiocarcinoma (CCA), however, the effects and mechanisms high glucose on progression CCA remain unclear. This study reports for first time enhancing aggressive phenotypes cells via STAT3 activation. cultured in media exerted significantly higher rates cell proliferation, adhesion, migration invasion than those normal glucose. The phosphokinase array revealed dominant signal activated response to...
To establish and characterize the gemcitabine-resistant cholangiocarcinoma (CCA) cell lines, CCA KKU‑M139 KKU‑M214 lines were exposed stepwisely to increasing gemcitabine (GEM). The resultant drug-resistant KKU‑M139/GEM KKU‑M214/GEM, retained resistant phenotype in drug-free medium at least for 2 months. Sulforhodamine B assay demonstrated that KKU‑M214/GEM 25.88- 62.31-fold more than their parental cells. Both cross-resistant 5-fluorouracil (5-FU), doxorubicin paclitaxel indicating...
Cholangiocarcinoma (CCA) is a major cause of cancer deaths in northeast Thailand. It aggressive, highly metastatic, and responds poorly to traditional chemotherapy. We demonstrated the potential for Cepharanthine (CEP), biscoclaurine alkaloid extracted from Stephania cepharantha , treat CCA. CEP significantly inhibited growth human CCA cell lines dose‐ time‐dependent manner, regardless histologic type tumor origin. Increasing apoptosis via caspase‐3 capase‐9 activation was CEP‐treated cells....
Cholangiocarcinoma (CCA) is an aggressive cancer for which standard treatments are still ineffective. This study demonstrated the antiproliferative and anti-metastatic activity of metformin, anti-diabetic drug, in CCA cells.Cell proliferation, migration/invasion anoikis resistance were determined. The underlying mechanisms identified using western blotting immunocytofluorescence.Metformin significantly suppressed proliferation cells a dose- time-dependent manner, regardless glucose present...
Abstract O-GlcNAcylation, an O-linked protein glycosylation with a single molecule of N-acetylglucosamine (GlcNAc), is reversibly controlled by O-GlcNAc transferase (OGT) and N-acetyl D-glucosaminidase (OGA). Aberrant O-GlcNAcylation contributes important role in initiation progression many human cancers. Elevation tumor tissues poor prognosis cholangiocarcinoma (CCA) patients have been reported. In this study, the promoting was further investigated CCA cell lines. Suppression using small...
Clear cell renal carcinoma (ccRCC) is the most common subtype of (RCC). Due to lack symptoms until advanced stages, early diagnosis ccRCC challenging. Therefore, identification novel secreted biomarkers for detection urgently needed. This study aimed identify diagnosing using bioinformatics and machine learning techniques based on transcriptomics data. Differentially expressed genes (DEGs) in compared normal kidney tissues were identified 3 datasets (GSE53757, GSE40435 GSE11151) from Gene...
O‐GlcNAcylation is an important biological process in regulating the function of many nucleocytoplasmic proteins cells. Enhancement was associated with cancer development and progression. Here, we demonstrated involvement melanoma metastasis. Using data from GEO database, found that its related enzymes, including glutamine fructose‐6‐phosphate amidotransferase (GFAT), O‐GlcNAc transferase (OGT), O‐GlcNAcase (OGA); were elevated metastatic compared primary tumors normal tissues. Functional...
Alteration of glycosylation in cancer cells leads to the expression tumor-associated glycans, which can be used as biomarkers for diagnosis and prognostic prediction diseases. In this study, we nano-LC-QToF identify serum N-glycan detection brain tumors. We observed an increase sialylated N-glycans a decrease fucosylated patients with glioblastoma (GBM) meningioma (MG) compared healthy individuals. GBM, combination increased (6_4_0_2 compound) decreased (4_4_1_0 was identified most...
Up-regulation and association of nuclear factor kappa B (NF-κB) with carcinogenesis tumor progression has been reported in several malignancies. In the current study, expression NF-κB cholangiocarcinoma (CCA) patient tissues its clinical significance were determined. The possibility using as therapeutic target CCA was demonstrated.Expression determined immunohistochemistry. Dehydroxymethylepoxyquinomicin (DHMEQ), a specific inhibitor, used to inhibit action. Cell growth an MTT assay, cell...
Lactate dehydrogenase A (LDHA), a key metabolic enzyme, plays crucial role in the final step of anaerobic glycolysis. Overexpression LDHA is observed many human malignancies association with tumor progression. The purpose this study was to investigate expression pattern during carcinogenesis, its clinico-pathological association, and evaluate prognostic value CCA patients. investigated using immunohistochemistry technique both hamster- (n=60) human-CCA tissues (n=82). Plasma LDH from healthy...
Berberine is a natural compound found in several herbs. Anticancer activity of berberine was reported cancers, however, little known regarding the effects against cholangiocarcinoma (CCA). In this study, growth inhibitory on CCA cell lines and its molecular mechanisms were explored. Cell cycle distribution examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay flow cytometry. The expression levels regulatory proteins determined Western blot analysis....
Pyruvate kinase M2 (PKM2) is an enzyme that predominantly overexpressed in various types of cancer. The role PKM2 liver fluke-associated cholangiocarcinoma (CCA) remains unclear. This study aimed to investigate the antitumor activity shikonin, a inhibitor, CCA cells.Immunohistochemistry and immunoblotting were used determine expression tissues cells. Antiproliferative effects shikonin evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony-formation trypan blue...
Renal cell carcinoma (RCC), accounting for 90% of all kidney cancer, is categorized into clear RCC (ccRCC) and non-clear (non-ccRCC) treatment based on the current NCCN Guidelines. Thus, classification will be associated with therapeutic implications. This study aims to identify novel biomarkers differentiate ccRCC from non-ccRCC using bioinformatics machine learning. The gene expression profiles subtypes (including papillary (pRCC) chromophobe (chRCC)), were obtained TCGA. Differential...
Up-regulation of vitamin D receptor (VDR) expression has been shown in several tumors and is thought to represent an important endogenous response tumor progression. The authors aimed verify the VDR its clinical significance histologically proven cholangiocarcinoma (CCA).The antiproliferative activity D3 on CCA cell lines was explored. immunohistochemistry 111 paraffin-embedded tissues showed that gradually increased during development. Normal bile duct epithelium rarely expresses VDR,...
Cholangiocarcinoma (CCA) is a dismal cancer. At present, there no effective chemotherapeutic regimen for CCA. This may be due to the marked resistance of CCA chemotherapy drugs, which mechanism remains unknown. Nuclear factor-κB (NF-κB) constitutively activated in variety cancer cells, including It has been shown play roles growth, metastasis, and chemoresistance In present study, we examined whether NF-κB involved dehydroxymethylepoxyquinomicin (DHMEQ), an inhibitor, can overcome drug Two...
AIM:To determine whether expression of certain enzymes related to 5-fluorouracil (5-FU) metabolism pre-dicts 5-FU chemosensitivity in cholangiocarcinoma (CCA). METHODS:The histoculture drug response assay (HDRA) was performed using surgically resected CCA tissues.Tumor cell viability determined morphologically with hematoxylin and eosin-and terminal deoxynucleotide transferase-mediated dUTP nick-end labeling-stained tissues.The mRNA thymidine phosphorylase (TP), orotate phosphoribosyl...