Epidemiology and animal models suggest that dietary monosodium glutamate (MSG) may contribute to the onset of obesity metabolic syndrome.Families (n = 324) from a rural area Thailand were selected provided MSG as sole source for use in meal preparation 10 days. Three hundred forty-nine subjects aged 35-55 years completed study evaluated energy nutrient intake, physical activity, tobacco smoking. The prevalence overweight (BMI ≥ 25 kg/m2), insulin resistance (HOMA-IR >3), syndrome (ATP III...

Cervical cancer (CxCa) is the most common in women and a prominent cause of mortality worldwide. The primary CxCa human papillomavirus (HPV). Radiation therapy chemotherapy have been used as standard treatments, but they undesirable side effects for patients. It was reported that gallic acid has antioxidant, antimicrobial, anticancer activities. Gold nanoparticles are currently being medicine biosensors drug delivery agents. This study aimed to develop agent using gold conjugated with acid....

Abstract Background It has been known that hypertension is an independent risk factor for cardiovascular disease (CVD). CVD the major cause of morbidity and mortality in developed developing countries. Elevation blood pressure (BP) increases adverse effect outcomes. Prevention increased BP plays a crucial role reduction those outcomes, leading to decrease mortality. Therefore, purpose this study was investigate effects dietary black sesame meal on oxidative stress individuals with...

Abstract Background Oxidative stress, an excess of reactive oxygen species (ROS), causes lipid peroxidation resulting in cell and tissue damages. It may be associated with the development progression cancers dogs. Malondialdehyde (MDA), end product peroxidation, is commonly used as a marker oxidative stress. The objective this study was to assess stress cancer-bearing dogs by measuring serum MDA levels. All client-owned underwent physical examination at Veterinary Teaching Hospital, Faculty...

Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), needed. Gold nanoparticles (AuNPs) used delivery agents. This study aimed to conjugate characterize AuNP-GA evaluate activity. AuNP was conjugated GA, core-shell structures were characterized by small-angle X-ray scattering...

Abstract Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) formers. Whether it has pathogenic role(s) in kidney formation or only entrapped inside remains unclear. We thus evaluated differences between E. patients with (EUK) that urinary tract infection (UTI) without (EUU). From 100 formers 200 UTI patients, four pairs EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine...

Abstract: The liver fluke, Opisthorchis viverrini, is the risk factor of cholangiocarcinoma, which a major health problem in northeastern Thailand. Production reactive oxygen and nitrogen species during host's response leads to oxidative nitrosative stress contributing carcinogenesis. We investigated protective effect melatonin against O. viverrini-induced injury. Hamsters were infected with viverrini followed by oral administration various doses (5, 10, 20 mg/kg body weight) for 30 days....

The current trend worldwide is searching plant extracts towards prevention of neurodegenerative disorders. This study aimed to investigate the neuroprotective effect Alpinia galanga leaves (ALE), rhizomes (ARE), Vitis vinifera seeds (VSE), Moringa oleifera (MLE), Panax ginseng (PLE) and (PRE) ethanolic on human neuroblastoma (SHSY5Y) cells. 1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging VSE MLE were 81% 58%, respectively. Ferric-reducing antioxidant power (FRAP) ALE (33.57 ± 0.20...

Phytochemicals play an important role in the decrease risk of free radicals associated diseases. In present study, total phenolic contents, antioxidant, antibacterial and anticancer activities thirty Thai vegetables have been evaluated. The results showed that contents were highly correlated with antioxidant activities. Four strains of Spondias pinnata Kurz., Careya sphaerica Roxb., Caesalpinia mimosoides Lamk. and Cratoxylum Subsp. Pruniflorum (Kurz.) Gogel. with...

Cervical cancer (CxCa) is a major health problem globally and associated with the presence of human papillomavirus infection. Cisplatin (CDDP) platinum-based chemotherapeutic agent. Owing to its side effects drug-resistance, novel anticancer agents lower toxicity, including caffeic acid (CFC), are interest. However, CDDP CFC in combination are, best our knowledge, uninvestigated. The present study investigated effectiveness mechanism action on four cervical cell lines, which were compared...

Abstract The anticancer activity of cationic antimicrobial peptides (AMPs) has become more interesting because some AMPs have selective recognition against cancer cells. However, their antitumor properties and underlying mechanisms in cells not been clearly understood. In this study, we evaluated the effects KT2 (lysine/tryptophan‐rich AMP) on cellular uptake internalization mechanism, cell viability, surface charge membrane, membrane integrity, apoptotic death, autophagy human HCT 116 colon...

Although cholangiocarcinoma (CCA) has a low incidence globally, this is extremely high in Northeast Thailand. The lack of both early detection measures and effective therapeutic drugs the major problem for poor prognosis CCA patients. Based on regional knowledge, it would be advantageous to search natural phyto-products treatment CCA. Cardiospermum halicacabum L., Gomphrena celosioides Mart. Scoparia dulcis very well-known medicinal herbs Asian countries, were selected investigation...

Chitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407) as gelling agents for oral drug delivery. The performance interaction mucin of chitosan-composed gel (F1) PVP-composed (F2) was compared using attenuated total reflectance–Fourier-transform infrared (ATR-FTIR) spectroscopy at controlled temperatures 25 37 °C 1 5 min. F1 containing niosome-entrapped melatonin its derivatives investigated mucoadhesive on mucosa by...

The human liver fluke Opisthorchis viverrini infection and N-nitrosodimethylamine (NDMA) administration induce cholangiocarcinoma (CCA) injury in hamsters. Melatonin protects against reduces the alteration of mitochondrial structure, membrane potential, pro- anti-apoptotic pathways various cancer types. To investigate chemopreventive effect melatonin on CCA genesis injury, hamsters were treated with a combination O. NDMA concurrently administered (10 mg/kg 50 mg/kg) for 120 days. treatment...

Extracts of Caesalpinia mimosoides Lamk has been reported to possess anticancer effects, but the active ingredients and anti-cancer mechanisms are still unknown.The effects a C extract on cell proliferation apoptosis induction in human cervical carcinoma lines, namely HeLa, SiHa, C33A, as well normal Vero cells, were investigated.Treatment with 5 fractions (F17-F21) methanol extracts inhibited viability dose- time- dependent manner. Neutral red assays indicated that treatment F21...

Cholangiocarcinoma (CCA) is a fatal cancer with poor prognosis and less than 10% of CCA patients can be offered surgical cure.Conventional chemotherapy results in unfavorable outcomes.At present, plant-derived compounds are gaining interest as potential therapeutics, particularly for treatment-refractory cancers.In this study, antitumor activity tiliacorinine, the major alkaloid isolated from tropical plant, on was first demonstrated.Antiproliferative effects tiliacorinine human cell lines...

Antidesma thwaitesianum Müll. Arg. or mao is widely used as commercial products of juice and wine in Thailand. As a result, waste from the plant, such seeds (MS) marcs (MM), are plentiful. We aimed to purify analyze polyphenolic content both MS MM investigate radical scavenging activities these polyphenolics against 1,1-diphenyl-2-picryl-hydrazyl (DPPH) 2,2'-Azinobis (3-ethylbenzothiazoline 6-sulphonate) (ABTS) radicals thiobarbituric acid reactive (TBARP). The results showed be an abundant...

Abstract Background Phytochemical products have a critical role in the drug discovery process. This promising possibility, however, necessitates need to confirm their scientific verification before use. Hence, this study aims evaluate (1) antioxidant activity, (2) cytotoxicity potential, and (3) effect on ultrastructural alteration gastric cancer cell lines through exposure fractions of three local Northeastern Thai edible plants. Methods Plants, Syzygium gratum, Justicia gangetica...

Cervical cancer is the third highest cause of death in developing countries and most commonly results from high‑risk human papillomavirus (HR‑HPV) infection. Among HR‑HPV genotypes, HPV16 HPV18 are prevalent cervical cancers. Therefore, present study aimed to develop a detection assay for infection using loop‑mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) tests. This simplified, user‑friendly method visual HPV genotypes. DNA was extracted clinical tissue samples,...

Gallic acid was isolated from Caesalpinia mimosoides Lamk and the structure s identified based on spectroscopic analysis comparison with authentic compound. In this study we compared ability of natural gallic (nGA) commercial (cGA) to inhibit proliferation cholangiocarcinoma cell lines (M213, M214) foodborne pathogenic bacteria (Salmonella spp. Plesiomonas shigelloides). Both nGA cGA had same inhibitory effects by inducing apoptosis lines. addition, inhibited growth in manner as cGA. Our...