Silver nanoparticles (AgNPs) have a strong antimicrobial activity against variety of pathogenic bacteria. The killing mechanism AgNPs involves direct physical membrane destruction and subsequent molecular damage from both released Ag+. Burkholderia pseudomallei is the causative agent melioidosis, an endemic infectious disease primarily found in northern Australia Southeast Asia. B. intrinsically resistant to most common antibiotics. In this study, (10–20 nm) were investigated. MIC MBC for...

Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), needed. Gold nanoparticles (AuNPs) used delivery agents. This study aimed to conjugate characterize AuNP-GA evaluate activity. AuNP was conjugated GA, core-shell structures were characterized by small-angle X-ray scattering...

Melioidosis is an infectious disease caused by Gram-negative bacillus bacteria Burkholderia pseudomallei. Due to the emerging resistance of B. pseudomallei antibiotics including ceftazidime (CAZ), development novel and alternative modes treatment has become urgent issue. Here, we demonstrated ability synergistically increase efficiency through their combination with silver nanoparticles (AgNPs). Combinations four conventional CAZ, imipenem (IMI), meropenem (MER), gentamicin sulfate (GENT)...

The excessive use of antibiotics in both human and veterinary medicine has contributed to the development rapid spread drug resistance bacteria. Silver nanoparticles (AgNPs) have become a tool choice that can be used treat these resistant Several studies shown AgNPs antibacterial wound healing properties. In this study, we evaluated biological activity anisotropic develop an antimicrobial gel formulation for treating infections. We showed some (S2) effective against bacterial pathogens low...

Abstract The anticancer activity of cationic antimicrobial peptides (AMPs) has become more interesting because some AMPs have selective recognition against cancer cells. However, their antitumor properties and underlying mechanisms in cells not been clearly understood. In this study, we evaluated the effects KT2 (lysine/tryptophan‐rich AMP) on cellular uptake internalization mechanism, cell viability, surface charge membrane, membrane integrity, apoptotic death, autophagy human HCT 116 colon...

Antimicrobial resistance is a growing health concern. peptides are potential solution because they bypass conventional drug mechanisms. Previously, we isolated peptide from Crocodylus siamensis hemoglobin hydrolysate, which has antimicrobial activity and identified the main this mixture (QL17). The objective of work was to evaluate rationally modify QL17 in order to: (1) control its mechanism action through bacterial membrane disruption; (2) improve activity; (3) ensure it low cytotoxicity...

Gallic acid was isolated from Caesalpinia mimosoides Lamk and the structure s identified based on spectroscopic analysis comparison with authentic compound. In this study we compared ability of natural gallic (nGA) commercial (cGA) to inhibit proliferation cholangiocarcinoma cell lines (M213, M214) foodborne pathogenic bacteria (Salmonella spp. Plesiomonas shigelloides). Both nGA cGA had same inhibitory effects by inducing apoptosis lines. addition, inhibited growth in manner as cGA. Our...

A novel antibacterial peptide from Crocodylus siamensis haemoglobin hydrolysate (CHH) was characterized for antimicrobial activity.CHHs were hydrolysed 2 h (2 h-CHH), 4 (4h-CHH), 6 (6 h-CHH) and 8 (8 h-CHH). The h-CHH showed activity against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae Pseudomonas aeruginosa at concentrations of 20, 20 10 mg ml-1 (w/v) respectively. Fluorescent microscopy revealed that the had bactericidal E. coli P. aeruginosa. β-galactosidase assay...

Burkholderia pseudomallei is the causative pathogen of melioidosis and this bacterium resistant to several antibiotics. Silver nanoparticles (AgNPs) are an interesting agent develop solve bacterial resistance. Here, we characterize assess antimelioidosis activity AgNPs against these pathogenic bacteria. were characterized displayed a maximum absorption band at 420 nm with spherical shape, being well-monodispersed having high stability in solution. The average size 7.99 ± 1.46 nm....

Cutibacterium acnes is one of the common multifactorial causes that play an important role in pathophysiology acne vulgaris. We aimed to develop novel antimicrobial peptides for reduction hypercolonization.Six cationic were derived by de novo designation. The and cytotoxic activities investigated. peptide conformation was determined circular dichroism spectrometry. effects evaluated using scanning electron microscopy (SEM), transmission (TEM) DNA-binding ability assay.Among designed...

Aim: The objective of this study was to analyze salivary lysozyme levels and activities in Thai preschoolers with different dental caries status. Study design: Unstimulated saliva samples were collected from 64 preschoolers, divided into a free group (n = 32) severe early childhood (S-ECC) 32). Results: Both groups similar regarding gender, age, status, flow rate, protein concentrations. No differences also the caregivers′ characteristics, oral health behaviors, feeding habits. Only...

New selective, efficacious chemotherapy agents are in demand as traditional drugs display side effects and face growing resistance upon continued administration. To this end, bioactive molecules such peptides attracting interest. RT2 is a cationic peptide that was used an antimicrobial but being repurposed for targeting cancer. In work, we investigate the mechanism by which targets Caco-2 human colon cancer cells, one of most prevalent metastatic cancers. Combining label-free proteomics with...

Many antimicrobial peptides have been shown to anticancer activity against human cancer cell lines. Cationic KT2 peptide, derived from white blood extract of Crocodylus siamensis has antibacterial and antitumor cervical cells, but there are no data on the effect peptide tumor growth in vivo. The colon xenografts was investigated nude mice.Tumors mice (BALB/c -nu/nu mice) were induced by subcutaneous injection with HCT116 cells. Twelve days after xenograft, treated intratumoral...

Wasp venom is a complex mixture containing proteins, enzymes and small molecules, including some of the most dangerous allergens. The greater banded wasp (Vespa tropica) well-known for its lethal venom, whose one major components hyaluronidase (HAase). It believed that high protein proportion activity this enzyme responsible potency.In present study, cDNA cloning, sequencing 3D-structure Vespa tropica HAase were described. Anti-native antibody was used neutralization assay.Two isoforms,...