Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation squamous epithelial cells at uterine cervix. The E2 protein is a key transcriptional regulator genes. It binds to four binding sites (E2BSs 1-4) in viral upstream regulatory region (URR). Modification functions, for example, by methylation E2BSs hypothesized enhanced oncogenes. In majority HPV-transformed premalignant lesions and about half cervical carcinomas genomes persist an extra-chromosomal,...

Dengue, a viral disease transmitted by Aedes mosquitoes, is an important public health concern throughout Thailand. Climate variables are potential predictors of dengue transmission. Associations between climate and have usually been performed on large-scale first-level national administrative divisions, i.e. provinces. Here we analyze data finer spatial resolution in one province, which often more relevant for effective control design. The objective this study was to investigate the effect...

In the early stages of human papillomavirus (HPV) infection, viral proteins elicit specific immune responses that can participate to regression ano-genital lesions. HPV E6 protein for instance reduce type I interferon (IFN) including IFN-κ is involved in evasion and persistence. To evaluate role E2 innate immunity HPV16-associated cervical lesions, genome-wide expression profiling primary keratinocytes (HPK) transduced by HPV16 was investigated using microarrays associated genes were...

Background Besides the well‐known risk factors, Epstein‐Barr virus ( EBV ) might play a significant role in oral squamous cell carcinoma OSCC ). To explore of , prevalence infection exfoliated cells cases and controls northeastern Thailand was investigated, association tumor lesion further confirmed. Methods Oral were collected from non‐cancer controls. Cells lesions taken patients for strong confirmation with . DNA detected by polymerase chain reaction PCR using primers specific polymerase....

Dengue is linked with climate change in tropical and sub-tropical countries including the Lao People's Democratic Republic (Laos) Thailand. Knowledge about these issues preventive measures can affect incidence outbreak risk of dengue. Therefore, present study was conducted to determine knowledge, attitudes, practices (KAP) among urban rural communities government officials dengue Laos Thailand.A cross-sectional KAP survey were 360 households (180 180 rural), 359 Thailand (179 20 (10 each...

Aedes aegypti is the main vector of dengue globally. The variables that influence abundance vectors are numerous and complex. This has generated a need to focus on areas at risk disease transmission, spatial-temporal distribution vectors, factors modulate abundance. To help guide improve vector-control efforts, this study identified ecological, social, other environmental affect adult female immature Ae. in households urban rural northeastern Thailand. A one-year entomological was conducted...

Mapping the spatial distribution of dengue vector Aedes (Ae.) aegypti and accurately predicting its abundance are crucial for designing effective control strategies early warning tools epidemic prevention. Socio-ecological landscape factors influence Ae. abundance. Therefore, we aimed to map female adult predict in northeastern Thailand based on socioeconomic, climate change, knowledge, attitude practices (KAP) and/or using machine learning (ML)-based system.A total 1066 females were...

MicroRNA-21 (miR-21) is recognized as an oncomir and shows up-regulation in many types of human malignancy. The aim this study was to investigate the association miR-21 expression associated with HPV infection normal abnormal cervical tissues. Cervical tissue samples different cytological or histopathological grades were investigated for by PCR programmed cell death, protein 4 (PDCD4) using quantitative real-time (qRT-PCR). Laser capture microdissection (LCM) stromal epithelial tissues situ...

MicroRNAs (miRNAs) are small non-coding RNAs that function to down-regulate gene expression involving in various cellular processes related carcinogenesis. Recently, miR-22 was identified as a tumor-suppressing miRNA many human cancers. However, the regulatory mechanism and specific of this cervical cancer remain unclear. In present study, we carried out transfection, western blot quantitative RT-PCR explore functional role cancer. We verified down-regulated tissues cell lines relative...

Cervical cancer is a serious public health problem in Thailand. We investigated possible risk factors for cervical including HPV infection, p53 polymorphism, smoking and reproductive history among women Northeast Thailand using case control study with 177 cases age-matched controls. Among the carriers, significantly increased an OR of 36.97(p<0.001) adjusted 38.07(p<0.001) were observed. Early age at first sexual exposure, multiple partners ORs ranging between 1.73-2.78(p<0.05). The interval...

Andrographolide (AD), and 14-deoxyandrographolide (DAD) isolated from Andrographis paniculata Nees, Acanthaceae, 3,19-isopropylideneandrographolide (IPAD), a semi-synthetic compound of AD, were examined for anti-HSV-1 activity in vitro. The inhibitory effects these compounds on viral entry replication steps determined using pre- post-infection assays, respectively. All the three exhibited less than 50% act against entry. In post-infection, IPAD displayed absolute inhibition, whereas AD DAD...

Abstract Background The variation of human papillomavirus (HPV) genes or HPV variants demonstrates different risks cervical cancer. Mutation in the long control region (LCR) at YY1-motifs is one mechanisms for enhancing viral oncogene expression during course cancer cell progression. In Thai women, cancers are almost always associated with HPV16 variant sub-lineage Asian (HPV16As); however, mechanism involved remains elusive. aim this study was to understand further oncogenic potential...

Arecoline, the major alkaloid of areca nut, is known to induce oral carcinogenesis, however, its mechanism still needed elucidate. This study investigated effects arecoline on cell viability and cell-cycle progression squamous carcinoma (OSCC) cells as well a relevant cellular gene expression. The results showed that low concentration (0.025 μg/ml) increased OSCC viability, proportion in G2/M phase proliferation. Simultaneously, it induced IL-6, STAT3 c-Myc Interestingly, c-myc promoter...

Background. Assessment of DNA methylation specific genes is one approach to the diagnosis cancer worldwide. Early stage detection necessary reduce mortality rate cancers, including those occurring in stomach. For this purpose, tumor cells circulating blood offer promising candidates for non-invasive diagnosis. Transcriptional inactivation suppressor genes, like PCDH10 and RASSF1A , by associated with progression gastric cancer, such can therefore be utilized as a biomarker. Methods. The...

Abstract The anticancer activity of cationic antimicrobial peptides (AMPs) has become more interesting because some AMPs have selective recognition against cancer cells. However, their antitumor properties and underlying mechanisms in cells not been clearly understood. In this study, we evaluated the effects KT2 (lysine/tryptophan‐rich AMP) on cellular uptake internalization mechanism, cell viability, surface charge membrane, membrane integrity, apoptotic death, autophagy human HCT 116 colon...

Human papillomavirus (HPV) is a major cause of cervical cancer. More than 100 HPV genotypes have been identified; however the distribution varies geographically and according to ethnicity. The purpose this study was investigate prevalence subtypes among Northeast Thai women. Subjects included 198 cases SCCA age-matched, healthy controls. HPV-DNA amplified by PCR using consensus primers GP5+/6+ system followed reverse line blot hybridization genotyping. high-risk infection 21 (10.1%) 152...

Atypical bacterial pathogens--including Mycoplasma pneumoniae, Chlamydophila and Chlamydia trachomatis--are important infectious agents of the respiratory system. Most current information pertains to adults little is known about role these organisms in lower tract infections among young children with acute bronchiolitis.This study detected pathogens nasopharyngeal secretions between 1 month 2 years age admitted bronchiolitis hospitals Khon Kaen, northeast Thailand. The M pneumoniae C were...

The Epstein-Barr virus (EBV) is a human herpesvirus associated with various cancers. number of reports that describe infection EBV in oral squamous carcinoma cells increasing. However, there no available vitro model to study the possible role development cell carcinoma. Herein, we report establishment latent well-differentiated HSC1 and poorly differentiated SCC25 cells. Viral copy numbers per EBV-infected are 2 5, respectively. Although was small, spontaneous viral replication observed...