A5005941126- Cervical Cancer and HPV Research
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Genital Health and Disease
- MicroRNA in disease regulation
- Head and Neck Cancer Studies
- Ovarian cancer diagnosis and treatment
- Virus-based gene therapy research
- Circular RNAs in diseases
- Reproductive tract infections research
- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Plant Disease Resistance and Genetics
- Extracellular vesicles in disease
- Colorectal and Anal Carcinomas
- Cancer-related gene regulation
- Hepatitis B Virus Studies
- Urological Disorders and Treatments
- Viral-associated cancers and disorders
- Protease and Inhibitor Mechanisms
- RNA modifications and cancer
Ministry of Health of the Russian Federation
2019-2024
Ministry of Health
2024
Tomsk National Research Medical Center
2022
Russian Cancer Research Center NN Blokhin
2015-2021
National Medical Research Center of Cardiology
2019
Heidelberg University
2004-2015
German Cancer Research Center
2011-2015
Zero to Three
2015
University Hospital Heidelberg
2011-2012
In-Q-Tel
2012
Chromosomal integration of high-risk human papillomavirus (HR-HPV) genomes is believed to represent a significant event in the pathogenesis cervical cancer associated with progression from preneoplastic lesions invasive carcinomas. This hypothesis based on experimental data suggesting that integration-dependent disruption HR-HPV E2 gene functions important achieve neoplastic transformation and clinical gathered by analyzing induced (HPV) 16 18 revealed integrated viral genome copies vast...
Persistent infection with carcinogenic human papillomaviruses (HPV) causes the majority of anogenital cancers and a subset head neck cancers. The HPV genome is frequently found integrated into host invasive mechanisms how it may promote disease progression are not well understood. Thoroughly characterizing integration events can provide insights carcinogenesis. Individual studies have reported limited number sites in cell lines samples. We performed systematic review published HPV-related...
Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation squamous epithelial cells at uterine cervix. The E2 protein is a key transcriptional regulator genes. It binds to four binding sites (E2BSs 1-4) in viral upstream regulatory region (URR). Modification functions, for example, by methylation E2BSs hypothesized enhanced oncogenes. In majority HPV-transformed premalignant lesions and about half cervical carcinomas genomes persist an extra-chromosomal,...
Increasingly deregulated expression of the E6-E7 oncogenes high-risk human papillomaviruses (HR-HPVs) has been identified as major transforming factor in pathogenesis cervical dysplasia and derived cancers. The these genes epithelial stem cells first results chromosomal instability induces aneuploidy. It is speculated that this subsequently favors integration HR-HPV genomes into cellular chromosomes. This turn leads to viral fusion transcripts further enhanced oncoproteins. Chromosomal...
The p16INK4a gene product is overexpressed strongly in abnormal cervical epithelia and may serve as a valuable biomarker to identify cells smears or liquid-based cytology samples.The authors performed immunocytochemistry locate that expressed samples used nuclear scoring system based on several morphologic criteria interpret the degree of abnormality these cells.Among 108 were scored normal Papanicolaou-stained, parallel slides, any p16INK4a-positive observed 13 (12%), but only 1 (1%) was...
High risk human papillomaviruses are squamous epitheliotropic viruses that may cause cervical and other cancers. HPV replication depends on epithelial differentiation. Transformation of HPV-infected cells goes along with substantial alteration the viral gene expression profile preferentially occurs at transformation zones usually uterine cervix. Methylation genome affect regulatory features control transcription genome. Therefore, we analyzed methylation pattern HPV16 upstream region (URR)...
Dysplastic lesions of the vagina or vulva often occur in women who have a previous history cervical dysplasia. Most female lower genital tract are induced by infections with high-risk oncogenic human papillomaviruses (HR-HPVs), including HPV16 and HPV18. HR-HPV genomes frequently integrate into host cell chromosomes at random sites. We analyzed viral integration sites multiple metachronous from previously treated for HR-HPV-positive dysplasia cancer to determine whether emerged single common...
BACKGROUND. The identification of a small percentage high-grade cervical intraepithelial neoplasias (HGCIN) among patients with minor cytological abnormalities (atypical squamous cells undetermined significance [ASCUS] and/or low-grade lesions [LSIL] group) is major problem in cytology-based cancer screening. authors investigated the efficacy p16INK4a as biomarker to identify samples HGCIN those an ASCUS or LSIL result Papanicolaou cytology. METHODS. Consecutive liquid-based cytology...
Integration of human papillomavirus (HPV) DNA into the host genome is a frequent event in cervical carcinogenesis and reported to occur at randomly selected chromosomal sites. However, as databases are being up-dated continuously, knowledge based on sequenced viral integration sites also expands. In this study, viral-cellular fusion transcripts preselected group 74 carcinoma or intraepithelial neoplasia grade 3 (CIN3) biopsies harboring integrated HPV16, HPV18, HPV31, HPV33, HPV45 were...
Diffuse overexpression of p16(INK4a) in basal and parabasal cells cervical epithelium is a hallmark human papillomavirus-mediated transformation. Focal expression occasionally observed nondysplastic epithelium. In normal cells, triggers cell cycle arrest. However, undergoing transformation intraepithelial lesions actively proliferate. To prove that the different patterns , i.e., focal versus diffuse, reflect biologically entities, we hypothesized -positive epithelia displaying pattern do not...
BACKGROUND The human papillomavirus (HPV) E2 protein is a transcriptional repressor of the oncogenes E6/E7 and loss function considered key step in carcinogenesis. Integration HPV into host genome may disrupt gene. Furthermore, methylation CpG dinucleotides E2‐binding sites (E2BSs) upstream regulatory region interfere with repression E6 E7 by E2. authors hypothesized that status E2BS identifies subtypes type 16 (HPV16)‐associated oropharyngeal squamous cell cancers (OPSCC) association gene...
Chromosomal copy number alterations (CNAs) and human papillomavirus (HPV) DNA integration into the host genome are more frequent in invasive cervical cancers compared to precancers.However, relationship between CNAs viral is not well understood.We analyzed chromosomal HPV 17 biopsies from women diagnosed with intraepithelial neoplasia grade 3 (CIN3) 21 carcinoma.All samples were HPV16-positive.HPV was evaluated by sequencing of chimeric transcripts or hybrid capture reads.Chromosomal...
p16 INK4a immunohistochemical overexpression is an overall reliable surrogate marker of human papillomavirus (HPV)‐associated head and neck squamous cell carcinomas (HNSCC). However, cases ambiguous are regularly detected in the neck: expression can be observed non‐malignant tissue, such as tonsillar crypt epithelium a proportion branchial cleft cysts. Additionally, diverse patterns INK4 complicate interpretation “p16 ‐positivity”. These aspects impede unrestricted application diagnostic...
High-risk human papillomaviruses (HR-HPVs) can be detected in a proportion of non-melanoma skin cancers. Data on prevalence are inconclusive, but essential to estimate the relevance HR-HPV, particularly with regard prophylactic HPV vaccines for cancer prevention. papillomavirus DNA was investigated 140 lesions from 54 immunocompetent patients and 33 immunosuppressed renal allograft recipients. Expression p16INK4a, marker HR-HPV oncogene expression uterine cervix, p53 pRB evaluated...
High-risk human papillomaviruses (hr HPVs) may cause various cancers and associated premalignant lesions. Transformation of the host cells is triggered by overexpression viral oncogenes E6 E7 that deregulate cell cycle induce chromosomal instability. This process accompanied hypermethylation distinct CpG sites resulting in silencing tumor suppressor genes, inhibition E2 mediated control transcription as well deregulated expression microRNAs. Therefore, we hypothesized treatment with...
Abstract Tissue inhibitor of metalloproteinase‐2 (TIMP‐2) is an endogenous matrix metalloproteinases (MMPs). This multifunctional protein regulates activities MMPs and possesses growth promoting effect in cell culture, anti‐tumoral, anti‐apoptotic anti‐angiogenic effects animal model systems vivo . It has been shown that this gene downregulated cervical carcinomas. The mechanism inhibition TIMP‐2 expression remains obscure. We have examined whether aberrant DNA methylation the 5′CpG island...
Extracellular vesicles (EVs), including exosomes, are key factors of intercellular communication, performing both local and distant transfers bioactive molecules. The increasingly obvious role EVs in carcinogenesis, similarity molecular signatures with parental cells, precise selection high stability cargo molecules make exosomes a promising source liquid biopsy markers for cancer diagnosis. uterine cavity fluid, unlike blood, urine other body fluids commonly used to study EVs, is origin...