A5085903523- Reproductive Biology and Fertility
- Connective Tissue Growth Factor Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Glycosylation and Glycoproteins Research
- Peroxisome Proliferator-Activated Receptors
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Kruppel-like factors research
- Erythrocyte Function and Pathophysiology
- Analytical Chemistry and Chromatography
- Alzheimer's disease research and treatments
- Sphingolipid Metabolism and Signaling
- Chromatography in Natural Products
- Ovarian function and disorders
- Hops Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Endometriosis Research and Treatment
- Immune cells in cancer
University of California, Davis
2023-2024
Sonoma State University
2019
Washington State University
2010-2011
The current study was designed to investigate the actions of Anti-Müllerian Hormone (AMH) on primordial follicle assembly. Ovarian follicles develop from breakdown oocyte nests during fetal development for human and immediately after birth in rodents. AMH found inhibit assembly decrease initial pool size a rat ovarian organ culture. expression be primarily stromal tissue ovaries at this period development, suggesting stromal-epithelial cell interaction promote alterations transcriptome with...
glycoPATH integrates RNAseq transcriptomic and LC-MS/MS glycomic data, providing a platform to identify genes implicated in diseases involving N -glycan biosynthesis enabling the development of targeted therapeutics for these pathways.
Abstract Microglia are the immune cells in central nervous system (CNS) and become pro-inflammatory/activated Alzheimer’s disease (AD). Cell surface glycosylation plays an important role cells; however, N-glycosylation glycosphingolipid (GSL) signatures of activated microglia poorly understood. Here, we study comprehensively combined transcriptomic glycomic profiles using human induced pluripotent stem cells-derived (hiMG). Distinct changes patterns amyloid-β oligomer (AβO) LPS-treated hiMG...
Abstract Glycosphingolipids are a unique class of bioactive lipids responsible for lateral membrane organization and signaling found in high abundance the central nervous system. Using nanoflow MEA Chip Q/ToF mass spectrometry, we profiled intact glycosphingolipids elderly human brain region-specific manner. By chromatographic separation glycan ceramide isomers, determined gangliosides to be highest source heterogeneity between regions with expression a- b-series structures. Investigation...
Sphingolipids are an essential subset of bioactive lipids found in most eukaryotic cells that contribute to membrane biophysical properties and involved cellular differentiation, recognition, mediating interactions. The described nanoHPLC-ESI-Q/ToF methodology utilizes known biosynthetic pathways, accurate mass detection, optimized collision-induced disassociation, a robust nanoflow chromatographic separation for the analysis intact sphingolipids human tissue, cells, serum. was developed...
Primordial follicle assembly is a process that occurs when oocyte nests break down to form individual primordial follicles. The size of this initial pool follicles in part determines the reproductive lifespan female. Connective tissue growth factor (CTGF) was identified as potential regulatory candidate for previous microarray analysis development. current study examines effects CTGF and associated transforming beta 1 (TGFβ-1) on assembly. Ovaries were removed from newborn rat pups placed an...
Ovarian primordial follicles are critical for female reproduction and comprise a finite pool of gametes arrested in development. A systems biology approach was used to identify regulatory gene networks essential follicle Transcriptional responses eight different growth factors known influence were construct bionetwork genes involved rat Over 1,500 found be regulated by the various network analysis identified modules number signaling pathways cellular processes. set 55 as potential regulators...
Altered N-glycosylation of proteins on the cell membrane is associated with several neurodegenerative diseases. Microglia are an ideal model for studying glycosylation and neuroinflammation, but whether aberrant in microglia can be restored by diet remains unknown. Herein, we profiled N-glycome, proteome, glycoproteome human following lipopolysaccharide (LPS) induction to probe impact dietary gut microbe-derived fatty acids-oleic acid, lauric palmitic valeric butyric isobutyric propionic...
This publication describes a method for the quantification by high-performance liquid chromatography (HPLC) of resinous compounds known as α-acids found in freshly harvested, unprocessed hops. provides consistent, efficient, and accurate results well theories rationale involved HPLC development. A system quality checks was utilized validation numerous developmental variables. By starting with theoretical approach preparation, extraction, instrumental techniques then further developing these...
The current study was designed to investigate the actions of Anti-Mullerian Hormone (AMH) on primordial follicle assembly. Ovarian follicles develop from breakdown oocyte nests during fetal development for human and immediately after birth in rodents. AMH found inhibit assembly decrease initial pool size a rat ovarian organ culture. expression be primarily stromal tissue ovaries at this period development, suggesting stromal-epithelial cell interaction promote alterations transcriptome with...
Microglia are the immune cells in central nervous system (CNS) and become pro-inflammatory/activated Alzheimer's disease (AD). Cell surface glycosylation plays an important role cells; however, N-glycosylation glycosphingolipid (GSL) signatures of activated microglia poorly understood. Here, we study comprehensive combined transcriptomic glycomic profiles using human induced pluripotent stem cells-derived (hiMG). Distinct changes patterns amyloid-β oligomer (AβO) LPS-treated hiMG were...
Abstract Background A previous RNA‐seq study of human Alzheimer’s disease (AD) brain samples revealed significant upregulation FUT8 coding for α1‐6 fucosyltransferase in parahippocampal gyrus, suggesting a role and the core fucosylation it catalyzes early AD. Despite well‐recognized roles immune regulation, its significance microglial function, especially neurodegenerative conditions such as AD, has rarely been studied. Method Human microglia derived from induced pluripotent stem cells (iMG)...
Ovarian primordial follicles are critical for female reproduction and comprise a finite pool of gametes arrested in development. A systems biology approach was used to identify regulatory gene networks essential follicle Transcriptional responses eight different growth factors known influence were construct bionetwork genes involved Over 1500 found be regulated by the various network analysis identified modules number signaling pathways cellular processes. set 55 as potential regulators...