A5018593270- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Gut microbiota and health
- Helminth infection and control
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Drug Transport and Resistance Mechanisms
- Forensic Entomology and Diptera Studies
- Gallbladder and Bile Duct Disorders
- Clostridium difficile and Clostridium perfringens research
- S100 Proteins and Annexins
- Bacterial Infections and Vaccines
- Dialysis and Renal Disease Management
- Pharmacological Effects and Toxicity Studies
- Liver Disease Diagnosis and Treatment
- Trace Elements in Health
- Cancer Mechanisms and Therapy
- Parasitic Infections and Diagnostics
- Liver Disease and Transplantation
- Cannabis and Cannabinoid Research
- Insect and Pesticide Research
- Vitamin K Research Studies
- Vector-borne infectious diseases
- Insect symbiosis and bacterial influences
- Congenital heart defects research
- Parasitic Diseases Research and Treatment
Khon Kaen University
2013-2025
Centre for Research and Development
2010
Medical Diagnostic Laboratories (United States)
2010
To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance Opisthorchis viverrini metacercariae (OV MC) was investigated in fish obtained from 20 provinces northeastern Thailand between April 2011 February 2012. A cross-sectional survey conducted on 12,890 consisting 13 species randomly caught 26 rivers, 10 dams, 38 ponds/lakes. Fish, were collected each rainy winter seasons province. Fish identified, counted,...
ABSTRACT Strongyloides stercoralis infection is a neglected tropical disease with global distribution. Serodiagnosis sensitive method, but improving its performance and simplifying into point-of-care test (POCT) are needed. This study aimed to improve the diagnostic of serological tests using partially purified ratti antigen in an enzyme-linked immunosorbent assay (ELISA) immunochromatographic (ICT). Crude S. was by IgG affinity column partition flow-through, washing fraction (WF), elution...
To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC).Expression of ANXA1 protein was investigated liver tissues from patients with CCA HCC by immunohistochemistry. Its expression on differences stages tumor development hamster induced Opisthorchis viverrini N-nitrosodimethylamine. Moreover, mRNA assessed cell lines quantitative real-time polymerase chain reaction silencing gene using small interfering RNA.In human...
Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the of CBD on human cells and underlying mechanisms vitro vivo. The three cell lines (KKU-213B, KKU-100, KKU-055) were assessed using SRB assay, clonogenic extent cell-cycle arrest, 3D holotomography. Morphological changes examined transmission electron...
ABSTRACT. Human strongyloidiasis is one of the neglected tropical diseases caused by infection with soil-transmitted helminth Strongyloides stercoralis . Conventional stool examination, a method commonly used for diagnosis S. , has low sensitivity, especially in case light infections. Herein, we developed droplet digital polymerase chain reaction (ddPCR) assay to detect larvae and compared its performance real-time PCR examination techniques (formalin ethyl-acetate concentration technique...
Abstract We explored the impact of chronic Strongyloides stercoralis infection on gut microbiome and microbial activity in a longitudinal study. At baseline (time-point T0), 42 fecal samples from matched individuals (21 positive for strongyloidiasis 21 negative) were subjected to 16S-rRNA sequencing. Those at T0 (untreated then because COVID19 lockdowns) retested one year later (T1). Persistent these indicated strongyloidiasis: they treated with ivermectin four months (T2). Fecal T1 T2...
ABSTRACT. Infection with Strongyloides stercoralis is often asymptomatic but can be life-threatening in immunocompromised patients, which prevented by ivermectin (IVM) treatment. The efficacy of IVM has been reported to have lessened over time some regions as a consequence prolonged use and mass treatment campaigns. Ivermectin used Thailand for more than decade; therefore, we investigated the single dose (200 µg/kg) against strongyloidiasis northeastern Thailand. Fecal samples were collected...
A proteomic-based approach was used to search for potential markers in the plasma of hamsters which cholangiocarcinoma (CCA) induced by Opisthorchis viverrini infection and N-nitrosodimethylamine treatment. The proteins CCA-induced were resolved 1-D PAGE, digested trypsin, analyzed LC-MS/MS. From criteria protein ID scores >15 an overexpression at least three times across all time points, 37 selected. These overexpressed largely consisted signal transduction, structural, transport,...
Strongyloides stercoralis infection typically causes severe symptoms in immunocompromised patients. This can also alter the gut microbiota and is often found areas where chronic kidney disease (CKD) common. However, relationship between S. microbiome not understood fully. Recent studies have shown that dysbiosis plays an important role progression of CKD. Hence, this study aims to investigate association CKD patients.Among 838 volunteers from Khon Kaen Province, northeastern Thailand, 40...
Understanding gut bacterial composition and proteome changes in patients with early-stage chronic kidney disease (CKD) could lead to better methods of controlling the progression. Here, we investigated microbiome microbial functions S. stercoralis infection (strongyloidiasis) CKD. Thirty-five early stages (1-3) CKD were placed two groups matched for population characteristics biochemical parameters, 12 strongyloidiasis one group 23 uninfected other. From every individual, a sample their...
Detection of Strogyloides -specific IgG antibodies in urine and serum has been used diagnostic epidemiological studies on strongyloidiasis. However, the usefulness these assays assessing responses to anthelmintic treatment is unclear. Thus, we evaluated performance temporal profiles Strongyloides a cohort participants at baseline post-treatment. The were prospectively screened for parasitic infections by fecal examination [agar plate culture technique (APCT) formalin-ethyl acetate...
Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for and treatment CCA. Monitoring such could lead to discovery targets CCA treatment. Curcumin exerts anti-inflammatory anti-CCA activities partly through its protein-modulatory ability. To support potential use curcumin discover novel target molecules treatment, we used quantitative proteomic approach investigate effects on in an...
We investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (PBMCs) cocultured with adult liver flukes (Opisthorchis viverrini) for 6 to 24 h. PBMC-derived proteins were identified by two-dimensional electrophoresis mass spectrometry, cytokines/chemokines in supernatant assessed using a cytokine array. Exposure O. viverrini induced increases secretion proinflammatory cytokines, costimulating protein, adhesion molecules,...
Detection of parasite-specific IgG in urine is a sensitive method for diagnosis strongyloidiasis and gives similar accuracy to serum IgG. However, there are no data concerning detection subclass urine. To further explore the utility from samples, we evaluated diagnostic performance IgG4 compared with parasitological other immunological methods.The sera included proven (group 1, n = 93), parasitic infections 2, 40) parasite negatives 3, 93). The Strongyloides-specific using fecal examinations...
Intestinal parasitic infections can change gut microbiota and short-chain fatty acids (SCFAs). We aimed to study the interaction among Strongyloides stercoralis, human microbiota, serum SCFAs in a community. Fifty-two subjects Donchang sub-district, Khon Kaen Province, northeastern Thailand, were included based on specific inclusion exclusion criteria. Characteristics of participants matched between those positive for S. stercoralis infection alone (no other intestinal parasites; Ss+, n=26)...
Canine monocytic ehrlichiosis caused by Ehrlichia canis infection is a life-threatening vector-borne disease in dogs worldwide. Routine blood smear has very low sensitivity and cannot accurately provide quantitative result. Conventional PCR (cPCR) real-time (qPCR) are widely used as molecular methods for E. detection. qPCR but relies on standard curves of known samples. To overcome this difficulty, study developed new detection method, using droplet digital polymerase chain reaction (ddPCR)....
ABSTRACT. Ivermectin (IVM) is a widely used anthelmintic. However, with widespread use comes the risk of emergence IVM resistance, particularly in strongyloidiasis. Adenosine triphosphate (ATP)-binding cassette (ABC) transporter genes play an important role IVM-resistance mechanism. Here, we aimed to establish animal experimental model resistance by frequent treatment Strongyloides ratti subtherapeutic doses IVM, being evaluated expression levels ABC genes. Rats infected S. were placed...