A5051004823- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Cardiovascular Effects of Exercise
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Polymer Surface Interaction Studies
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Genetic Neurodegenerative Diseases
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Skin and Cellular Biology Research
- Genetics and Physical Performance
- Tissue Engineering and Regenerative Medicine
- Advanced Electron Microscopy Techniques and Applications
UNSW Sydney
2015-2024
Kensington Health
2024
Cancer Institute of New South Wales
2021
University College London
2016
Royal National Orthopaedic Hospital
2016
Neuroscience Research Australia
2014
Children's Hospital at Westmead
2003-2013
The University of Sydney
2001-2013
Monash University
1976-2013
Translational Research Institute
2013
cDNA clones encoding three classes of human actins have been isolated and characterized. The first two (gamma beta, cytoplasmic actins) were obtained from a library constructed simian virus 40-transformed fibroblast mRNA, the third class (alpha, muscle actin) was adult mRNA. A new approach developed to enrich for full-length cDNAs. plasmid linearized with restriction enzymes that did not cut inserts interest; it then size-fractionated on gels, chimeric molecules optimal length selected...
We report the complete nucleotide sequence of a human β actin cDNA. Both 5′ and 3′ untranslated regions are similar (>80%) to analogous rat β-actin gene reported by Nudel et al (1983) 1 . When segment region is used as radiolabelled probe, strong hybridization chick mRNA seen. This conservation sequences suggests that selective pressures operate on non-translated segments mRNA.
Metacognition, the ability to reflect upon, and evaluate our own beliefs, can help us avoid making decisions based on unreliable evidence. Here, we provide empirical tests of importance human metacognition during COVID-19 pandemic. ...Metacognition, manifests itself in confidence have these helps guide behavior complex uncertain environments. ...
cDNA clones encoding three classes of human actins have been isolated and characterized. The first two (gamma beta, cytoplasmic actins) were obtained from a library constructed simian virus 40-transformed fibroblast mRNA, the third class (alpha, muscle actin) was adult mRNA. A new approach developed to enrich for full-length cDNAs. plasmid linearized with restriction enzymes that did not cut inserts interest; it then size-fractionated on gels, chimeric molecules optimal length selected...
We have constructed isotype-specific subclones from the 3' untranslated regions of alpha-skeletal, alpha-cardiac, beta-cytoskeletal, and gamma-cytoskeletal actin cDNAs. These clones been used as hybridization probes to assay number organization these isotypes in human genome. Hybridization genomic (Engel et al., Proc. Natl. Acad. Sci. U.S.A. 78:4674-4678, 1981; Engel Mol. Cell. Biol. 2:674-684, 1982) has allowed unambiguous assignment isotypically defined subfamilies. In addition, only one...
We have assigned six members of the human beta-actin multigene family to specific chromosomes. The functional gene, ACTB, is located on chromosome 7, and other beta-actin-related sequences are dispersed over at least four different chromosomes including one locus X chromosome. Using intervening sequence probes, we showed that gene single copy all related recently generated in evolution probably processed pseudogenes. entire nucleotide has been determined identical cDNA clones coding 5'...
A common nonsense polymorphism (R577X) in the ACTN3 gene results complete deficiency of fast skeletal muscle fiber protein α-actinin-3 an estimated one billion humans worldwide. The XX null genotype is under-represented elite sprint athletes, associated with reduced strength and performance non-athletes, over-represented endurance suggesting that increases at cost power generation. Here we report from Actn3 knockout mice displays force generation, consistent human association studies....
Apoptosis is an important biological process required for the removal of unwanted or damaged cells. Mounting evidence implicates actin cytoskeleton as both a sensor and mediator apoptosis. Studies also suggest that binding proteins (ABPs) significantly contribute to apoptosis dynamics play key role in regulating signaling. Changes organization has been attributed malignant transformation it hypothesized remodeling may enable tumor cells evade normal apoptotic This review aims illuminate by...
The specific functions of greater than 40 vertebrate nonmuscle tropomyosins (Tms) are poorly understood. In this article we have tested the ability two Tm isoforms, TmBr3 and human homologue Tm5 (hTM5 NM1 ), to regulate actin filament function. We found that these Tms can differentially alter organization, cell size, shape. hTm5 was able recruit myosin II into stress fibers, which resulted in decreased lamellipodia cellular migration. contrast, transfection induced lamellipodial formation,...
When a clonal line of rat pheochromocytoma (PC12) was exposed to beta-nerve growth factor (beta NGF), N6, O2-dibutyryl adenosine 3':5' cyclic monophosphate (Bt2cAMP), or combination the two, 10, 26, 70% cell clumps, respectively, displayed neurites after 1.d. Increases in cellular RNA concentration were also found be additive greater when both agents present. Neurites induced by Bt2cAMP alone not maintained replacement with beta NGF. The degree potentiated neurite outgrowth function time...
We determined the actin isotypes encoded by 30 cDNA clones previously isolated from an adult human muscle library. Using 3' untranslated region probes derived alpha-skeletal, beta- and gamma-actin cDNAs alpha-cardiac genomic clone, we showed that 28 of correspond to alpha-skeletal transcripts. Unexpectedly, however, remaining two proved derive mRNA. Sequence analysis confirmed skeletal are transcripts cloned gene. Direct measurements isotype mRNA expression in is expressed at 5% level actin....
Frontotemporal dementia (FTD) is characterized by cognitive and behavioral changes and, in a significant subset of patients, Parkinsonism. Histopathologically, FTD frequently presents with tau-containing lesions, which familial cases result from mutations the MAPT gene encoding tau. Here we present novel transgenic mouse strain (K3) that expresses human tau carrying mutation K369I. K3 mice develop progressive histopathology reminiscent K369I mutation. In addition, show early-onset memory...
We have assigned six members of the human beta-actin multigene family to specific chromosomes. The functional gene, ACTB, is located on chromosome 7, and other beta-actin-related sequences are dispersed over at least four different chromosomes including one locus X chromosome. Using intervening sequence probes, we showed that gene single copy all related recently generated in evolution probably processed pseudogenes. entire nucleotide has been determined identical cDNA clones coding 5'...
Two distinct, but iso-coding. γ non-muscle actin cDNAs were isolated from an SV40-transformed human fibroblast library. The complete nucleotide sequence of the eDNAs indicates that they may have arisen polymorphic alleles. By using genomic DNA and cellular RNA transfer blots, we demonstrate 3′ untranslated region (UTR) mRNA consists evolutionanly conserved 5′ more divergent segments. In fact, segment UTR detects a single- copy in chicken genome 20S transcript tissues. coding regions these...
The accumulation of the cytoskeletal beta- and gamma-actin mRNAs was determined in a variety mouse tissues organs. beta-isoform is always expressed excess gamma-isoform. However, molar ratio to mRNA varies from 1.7 kidney testis 12 sarcomeric muscle 114 liver. We conclude that, whereas gamma-actins are truly coexpressed, their levels subject differential regulation between different cell types. human gene has been cloned sequenced, its chromosome location determined. located on 17, unlike...
Abstract The actin cytoskeleton is a potentially vulnerable property of cancer cells, yet chemotherapeutic targeting attempts have been hampered by unacceptable toxicity. In this study, we shown that it possible to disrupt specific filament populations isoforms tropomyosin, core component filaments, are selectively upregulated in cancers. A novel class anti-tropomyosin compounds has developed preferentially disrupts the tumor impairing both cell motility and viability. Our lead compound,...