A5032598119- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Click Chemistry and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Boron Compounds in Chemistry
- Adipose Tissue and Metabolism
- Biosensors and Analytical Detection
- Cell Adhesion Molecules Research
- Mass Spectrometry Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Molecular Sensors and Ion Detection
- Ion-surface interactions and analysis
- CO2 Reduction Techniques and Catalysts
- Radiopharmaceutical Chemistry and Applications
- Immunotherapy and Immune Responses
- Magnetism in coordination complexes
- Cardiovascular Disease and Adiposity
University of Strathclyde
2020-2025
Robert Bosch (Germany)
2022
University of Edinburgh
2016-2021
Edinburgh Cancer Research
2016-2019
University of Nottingham
2014
Carboxylesterases (CEs) are a class of enzymes that catalyze the hydrolysis esters in variety endogenous and exogenous molecules. CEs play an important role drug metabolism, onset progression disease, can be harnessed for prodrug activation strategies. As such, regulation is clinical pharmaceutical consideration. Here, we report first ratiometric sensor CE activity using Raman spectroscopy based on bisarylbutadiyne scaffold. The was shown to highly sensitive specific detection had low...
Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation a series Raman-active labels has allowed identification highly active which have minimal perturbation on biological efficacy parent drug. Drug uptake been correlated markers cellular composition and cell cycle status, mapped across structures using dual-colour multi-modal imaging. The phototoxicity...
Hyperspectral stimulated Raman scattering (SRS) microscopy is a powerful imaging modality for the analysis of biological systems. Here, we report application k-means cluster (KMCA) multi-wavelength SRS images in high-wavenumber region spectrum as robust and reliable method segmentation cellular organelles based on intrinsic spectrum. KMCA has been applied to study endogenous lipid biochemistry prostate cancer healthy cell models, while corresponding droplet (LD) enabled direct comparison...
Statins have displayed significant, although heterogeneous, anti-tumour activity in breast cancer disease progression and recurrence. They offer promise as a class of drugs, normally used for cardiovascular control, that could significant impact on the treatment cancer. Understanding their mode action accurately assessing efficacy live cells is an important challenge. Stimulated Raman scattering (SRS) microscopy powerful, label-free imaging technique can rapidly characterise biochemical...
Abstract Arterial Vascular smooth muscle cells (VSMCs) play a central role in the onset and progression of atherosclerosis. Upon exposure to pathological stimuli, they can take on alternative phenotypes that, among others, have been described as macrophage like, or foam cells. VSMC make up >50% all arterial suggested retain an even higher proportion cell stored lipid droplets, further leading apoptosis, secondary necrosis, inflammatory response. However, mechanism formation is still...
Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked several disease states. We report the design, synthesis, application of mitokyne 1—the first small molecule sensor stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in response external stimuli inhibition both electron transport chain ATP synthase with inhibitors. In addition, 1 was also used monitor dynamics a time-resolved...
Probes based on Alkyne Tag Raman Imaging offer an entirely new platform for the sensing of pH<sub>i</sub>.
Abstract Sucrose is the main saccharide used for long‐distance transport in plants and plays an essential role energy metabolism; however, there are no analogues real‐time imaging live cells. We have optimised a synthetic approach to prepare sucrose including very small (≈50 Da or less) Raman tags fructose moiety. Spectroscopic analysis identified alkyne‐tagged compound 6 as analogue recognised by endogenous transporters cells with higher intensity than other derivatives. Herein, we...
Abstract Multiplex optical detection in live cells is challenging due to overlapping signals and poor signal‐to‐noise associated with some chemical reporters. To address this, the application of spectral phasor analysis stimulated Raman scattering (SRS) microscopy for unmixing three bioorthogonal probes within reported. Triplex a metallacarborane using B−H stretch at 2480–2650 cm −1 , together bis ‐alkyne deuterated fatty acid can be achieved cell‐silent region spectrum. When coupled imaging...
Metallacarboranes have long been the subject of attention in context medicinal chemistry because their promising characteristics and unconventional interactions with biological entities. The metal centre has shown to a significant influence on internalisation cytotoxicity compounds human cell lines. Additionally, specific nanomolar concentrations metallacarboranes demonstrated toxic effects MDA-MB-231 cells under vitro vivo conditions.
Altered differentiation of blood cell precursors and their clonal expansion occurs in various types leukaemia. One treatment strategy is to induce into the mature form, which capable undergoing apoptosis. It has been found that low doses doxorubicin (DOX) phenotypic morphological changes malignant erythroid erythrocyte-like cells. These are usually studied by time-consuming examination haemoglobinisation benzidine staining or glycophorin A expression Western blot flow cytometry. As an...
A relatively new approach to subcellular research is Raman microscopy with the application of sensors called probes. This paper describes use sensitive and specific probe, 3-O-propargyl-d-glucose (3-OPG), track metabolic changes in endothelial cells (ECs). ECs play a significant role healthy dysfunctional state, latter correlated range lifestyle diseases, particularly cardiovascular disorders. The metabolism glucose uptake may reflect physiopathological conditions cell activity energy...
Hyperspectral stimulated Raman scattering (SRS) microscopy is a robust imaging tool for the analysis of biological systems. Here, we present unique perspective, label-free spatiotemporal map mitosis, by integrating hyperspectral SRS with advanced chemometrics to assess intrinsic biomolecular properties an essential process mammalian life. The application spectral phasor multiwavelength images in high-wavenumber (HWN) region spectrum enabled segmentation subcellular organelles based on innate...
Glioblastoma multiforme (GBM) is a particularly aggressive and high-grade brain cancer, with poor prognosis life expectancy, in urgent need of novel therapies. These severe outcomes are compounded by the difficulty distinguishing between cancerous non-cancerous tissues using conventional imaging techniques. Metallic nanoparticles (NPs) advantageous due to their diverse optical physical properties, such as targeting potential. In this work, uptake, distribution, location silica coated gold...
Metabolism of endothelial cells (ECs) depends on the availability energy substrates. Since endothelium is first line defence against inflammation in cardiovascular system and its dysfunction can lead to development diseases, it important understand how glucose metabolism changes during inflammation. In this work, uptake was studied human microvascular (HMEC-1) high (HG), additionally an inflammatory state, using Raman imaging. HG state induced by incubation ECs with a deuterated analogue,...
A simple ratiometric sensor based on Raman spectroscopy enables rapid fluoride ion detection in a paper-based assay using portable spectrometer.
Antagonism of αvβ6 is emerging as a potential treatment idiopathic pulmonary fibrosis based on strong target validation. Starting from an αvβ3 antagonist lead and through simple variation in the nature position aryl substituent, discovery compounds with improved activity described. The also have physicochemical properties commensurate oral bioavailability are high quality starting points for drug program. Compounds 33S 43E1 pan αv antagonists having ca. 100 nM potency against αvβ3, αvβ5,...
Stimulated Raman scattering (SRS) microscopy is a powerful technique for visualising the cellular uptake and distribution of drugs small molecules in live cells under biocompatible imaging conditions. The use bio-orthogonal groups within drug molecule, including alkynes nitriles, has enabled direct detection plethora bioactive minimally perturbative fashion. Limited progress been made towards real-time into physiological conditions, despite accordant potential it presents preclinical...