A5035485196- Spectroscopy Techniques in Biomedical and Chemical Research
- Acute Lymphoblastic Leukemia research
- Molecular Biology Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Virology and Viral Diseases
- Digital Imaging for Blood Diseases
- Radiopharmaceutical Chemistry and Applications
- Metabolomics and Mass Spectrometry Studies
- Infrared Thermography in Medicine
- Metabolism and Genetic Disorders
- Hepatitis B Virus Studies
- Cancer Diagnosis and Treatment
- Animal Virus Infections Studies
- Microfluidic and Bio-sensing Technologies
- S100 Proteins and Annexins
- Biosensors and Analytical Detection
- Hematopoietic Stem Cell Transplantation
- Advanced Fluorescence Microscopy Techniques
- Bacterial Infections and Vaccines
- Chemical Reactions and Isotopes
- Medical Imaging and Pathology Studies
- Cerebral Palsy and Movement Disorders
- Protein Interaction Studies and Fluorescence Analysis
- Optical Imaging and Spectroscopy Techniques
- Bone health and treatments
Jagiellonian University
2020-2023
Human peripheral blood mononuclear cells (PBMCs) are a heterogeneous population of that includes T and B lymphocytes. The total number lymphocytes their percentage in the can be marker for diagnosis several human diseases. Currently, cytometric methods widely used to distinguish subtypes leukocytes quantify number. These techniques use cell immunophenotyping, which is limited by fluorochrome-labeled antibodies applied simultaneously. were isolated from obtained healthy donors. immunomagnetic...
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) with chromosome translocations like
Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from progenitors B lymphocytes. Traditionally, risk factors stratifying therapy ALL patients included age at diagnosis, initial leukocytosis, and response to chemotherapy. Currently, treatment intensity modified according presence specific gene alterations leukemic genome. Raman imaging a promising diagnostic tool, which enables molecular...
Abstract Here we report a new Raman probe for cellular studies on lipids detection and distribution. It is (3S, 3'S)-astaxanthin (AXT), natural xanthophyll of hydrophobic properties high solubility in lipids. contains chromophore group, long polyene chain eleven conjugated C=C bonds including two the terminal rings, absorbing light visible range that coincides with excitation lasers commonly used spectroscopy studying biological samples. Depending laser, resonance (excitation range) or...
Endothelial cells (EC) constitute a single layer of the lining blood vessels and play an important role in maintaining cardiovascular homeostasis. dysfunction has been recognized as primary or secondary cause many diseases it manifests itself, among others, by increased lipid content change composition EC. Therefore, analysis cellular lipids is crucial to understand mechanisms disease development. Tumor necrosis factor alpha (TNF-α)-induced inflammation EC alters cells, which can be detected...
Endothelial dysfunction contributes to the pathophysiology of multiple civilization diseases. Biochemical changes dysfunctional endothelium are linked cellular organelles, including mitochondria, nucleus or lysosomes, so organelle-specific insight is needed for better understanding endothelial pathobiology. Raman imaging, combining chemical specificity with microscopic resolution, able detect biochemical at subcellular level. However, detection spectroscopic markers associated specific cell...