A5073999198- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Antimicrobial Peptides and Activities
- Plant Disease Management Techniques
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Luminescence and Fluorescent Materials
- Pharmacological Effects of Natural Compounds
- Mycotoxins in Agriculture and Food
- Chromatography in Natural Products
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Click Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Plant Pathogens and Fungal Diseases
- Peptidase Inhibition and Analysis
- Plant Pathogens and Resistance
- Hepatitis C virus research
- Polyamine Metabolism and Applications
- CRISPR and Genetic Engineering
- Protein Kinase Regulation and GTPase Signaling
- Fungal Plant Pathogen Control
Manchester Metropolitan University
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
North China University of Water Resources and Electric Power
2025
Nantong University
2022-2024
University of Manchester
2016-2024
Fujian Agriculture and Forestry University
2024
China Agricultural University
2014-2023
Genomics (United Kingdom)
2022
Centre for Inflammation Research
2020
Manchester Academic Health Science Centre
2019-2020
Abstract Fluorescent antimicrobial peptides are promising structures for in situ , real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly human pulmonary tissue. We have developed Trp-BODIPY amino acid with spacer-free C-C linkage between Trp and BODIPY fluorogen, which shows remarkable fluorescence enhancement hydrophobic microenvironments. The incorporation our short does not impair their selectivity cells, enables rapid direct...
Abstract Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show an endogenously produced secreted oxylipin, 5,8-diHODE, induces cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus flavus , appressorium formation the rice blast pathogen Magnaporthe grisea . The response is specific to a subset of oxylipins...
Aspergillus fumigatus is a saprophytic fungal pathogen that the cause of more than 300,000 life-threatening infections annually. Our understanding pathogenesis and factors contributing to disease progression are limited. Development rapid versatile gene editing methodologies for A. essential. CRISPR-Cas9 mediated transformation has been widely used as novel genome tool variety techniques, such protein tagging, deletions site-directed mutagenesis in fumigatus. However, successful relies on...
Fungal infections caused by Candida species are among the most prevalent in hospitalized patients. However, current methods for detection of fungal cells clinical samples rely on time-consuming assays that hamper rapid and reliable diagnosis. Herein, we describe rational development new Phe-BODIPY amino acids as small fluorogenic building blocks their application to generate fluorescent antimicrobial peptides labelling urine. We have used computational analyse behaviour BODIPY-substituted...
With the advent of antimicrobial resistance, there is an urgent need for new strategies to treat infectious diseases. Antimicrobial peptides are considered as promising candidates, and therefore a understand their mechanism action in order exploit therapeutic potential. To this end, fluorescent analogs powerful tools analyze behavior subcellular localization cells vivo. However, conjugation fluorophores peptides, especially short sequences, can impair biological activity, making selection...
Aspergillus fumigatus is a human pathogen and the principal etiologic agent of invasive chronic aspergillosis leading to several hundreds thousands deaths every year. Very few antifungals are available treat infections caused by A. fumigatus, resistance developing those we have. Our understanding molecular mechanisms that drive pathogenicity drug have been hampered lack large mutant collections, which limits our ability perform functional genomics analysis. Here present high-throughput gene...
Pathogen-pathogen interactions in polymicrobial infections are known to directly impact, often worsen, disease outcomes. For example, co-infection with Pseudomonas aeruginosa and Aspergillus fumigatus, respectively the most common bacterial fungal pathogens isolated from cystic fibrosis (CF) airways, leads a worsened prognosis. Recent studies of vitro microbial cross-talk demonstrated that P. aeruginosa-derived volatile sulfur compounds (VSCs) can promote A. fumigatus growth vitro. However,...
Sulfur metabolism is an essential aspect of fungal physiology and pathogenicity. Fungal sulfur comprises anabolic catabolic routes that are not well conserved in mammals, therefore considered a promising source prospective novel antifungal targets. To gain insight into Aspergillus fumigatus sulfur-related during infection, we used NanoString custom nCounter-TagSet compared the expression 68 key metabolic genes different murine models invasive pulmonary aspergillosis, at 3 time-points, under...
Small-scale, dispersed agroforestry spaces in the urban fringe constitute ecological land that serves dual public benefit functions: natural conservation and rural-urban services. The purpose of this study is to construct a green infrastructure network by integrating existing potential an fringe. Zhengzhou was chosen as site. First, identified based on multi-source data artificial intelligence, followed extraction elements through morphological spatial pattern analysis. Then, output...
To sustain iron homeostasis, microorganisms have evolved fine-tuned mechanisms for uptake, storage and detoxification of the essential metal iron. In human pathogen Aspergillus fumigatus, fungal-specific bZIP-type transcription factor HapX coordinates adaption to both starvation excess is thereby crucial virulence. Previous studies indicated that a homodimer interacts with CCAAT-binding complex (CBC) cooperatively bind bipartite DNA motifs; however, mode HapX-DNA recognition had not been...
Six novel mycoviruses that collectively represent the mycovirome of Rhizoctonia solani anastomosis group (AG)-3 PT strain ZJ-2H, which causes potato black scurf, were identified through metatranscriptome sequencing and putatively designated as fusarivirus 4 [RsFV4, positive single-stranded RNA (+ssRNA)], 5 (RsFV5, +ssRNA), mitovirus 40 (RsMV40, partitivirus 10 [RsPV10, double-stranded (dsRNA)], 11 (RsPV11, dsRNA), virus (RsRV11, dsRNA). Whole genome sequences RsFV4, RsMV40, RsPV10, RsPV11,...
A dual-enzyme-responsive indicator for the non-invasive diagnosis of bacterial and non-bacterial inflammations has been developed. This responds to NAD(P)H NTR in inflammations, emitting two distinct turn-on fluorescence signals.
More than 10 million people suffer from lung diseases caused by the pathogenic fungus Aspergillus fumigatus. Azole antifungals represent first-line therapeutics for most of these infections but resistance is rising, therefore identification antifungal targets whose inhibition synergises with azoles could improve therapeutic outcomes. Here, we generate a library 111 genetically barcoded null mutants fumigatus in genes encoding protein kinases, and show that loss function kinase YakA results...
Abstract N ‐Myristoyltransferase (NMT) is a cytosolic monomeric enzyme present in eukaryotes such as fungi and protozoa, but not found prokaryotes. The attachment of 14‐carbon saturated fatty acid, myristate, from myristoyl‐CoA (14:0 CoA) to the N‐terminal glycine residue specific set cellular proteins commonly called protein N‐myristoylation. myristoylation reaction catalyzed by myristoyl CoA:NMT both necessary for growth various organisms conclusive proliferation. Therefore, NMT has been...
Abstract Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While not problematic for single isolates, can be time consuming large numbers strains if using traditional procedures. Therefore, in article we describe fast efficient thermal‐shock method to release spores A. other filamentous fungi without the need methods. This especially important...
In this study, the diversity of putative mycoviruses present in 66 strains binucleate Rhizoctonia (BNR, including anastomosis group (AG)-A, AG-Fa, AG-K, and AG-W) 192 multinucleate (MNR, AG-1-IA, AG-2-1, AG-3 PT, AG-4HGI, AG-4HGII, AG-4HGIII, AG-5), which are causal agents potato stem canker or black scurf, was studied using metatranscriptome sequencing. The number contigs related to identified from BNR MNR 173 485, respectively. On average, each strain accommodated 2.62 mycoviruses, while...
The human Caco-2 cell monolayer model was used to investigate the absorption property, mechanism, and structure-property relationship of seven representative flavonoids, namely, orientin, vitexin, 2"-O-β-L-galactopyranosylorientin, 2"-O-β-L-galactopyranosylvitexin, isoswertisin, isoswertiajaponin, 2"-O-(2"'-methylbutanoyl)isoswertisin from flowers Trollius chinensis. results showed that these flavonoids were hardly transported through monolayer. compounds with 7-OCH3 including...