A5100655961- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Nanomaterials in Catalysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Carbon and Quantum Dots Applications
- CRISPR and Genetic Engineering
- Quantum Dots Synthesis And Properties
- Advanced Photocatalysis Techniques
- Selenium in Biological Systems
- Electrochemical sensors and biosensors
- Analytical Chemistry and Chromatography
- DNA and Nucleic Acid Chemistry
- Nanocluster Synthesis and Applications
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Microfluidic and Capillary Electrophoresis Applications
- Plant Virus Research Studies
- Nanoplatforms for cancer theranostics
- Pesticide Residue Analysis and Safety
- Copper-based nanomaterials and applications
- Alkaline Phosphatase Research Studies
- Advanced Chemical Sensor Technologies
- Pharmacological Effects and Assays
- Graphene research and applications
- Analytical Chemistry and Sensors
Ministry of Agriculture and Rural Affairs
2022-2025
Southwest University
2016-2025
Citrus Research Institute
2016-2025
Wuhan University
2010-2024
Ningbo Polytechnic
2024
Jilin University
2024
Nantong University
2024
Guangzhou Experimental Station
2023
Fudan University
2021
Jiangsu Provincial Center for Disease Control and Prevention
2021
Mucin 1 (MUC1) which presents in epithelial malignancies, is a well-known tumor biomarker. In this paper, highly sensitive and selective fluorescent aptasensor for detection constructed, utilizing graphene oxide (GO) as quencher can quench the fluorescence of single-stranded dye-labeled MUC1 specific aptamer. absence MUC1, adsorption aptamer on GO brings dyes close proximity to surface resulting high efficiency quenching dye fluorescence. Therefore, designed completely quenched by GO, system...
A sensitive colorimetric aptasensor has been developed based on structure-switching of an OTA aptamer coupled with alkaline phosphatase-MnO<sub>2</sub> cascade catalytic reaction.
Cardiotonic steroids (CTSs) are ancient and effective clinical drugs for treating heart failure. Glycosylation of the CTS, especially at C-3 position, is great significance in nowadays pharmacological applications because it can lead to increased solubility, reduced toxicity, expanded bioactivity CTS. Plant steroid 3-O-glycosyltransferase (S3GT) a practical tool achieve glycosylation However, S3GT with both substrate sugar donor promiscuity remains unidentified. Mechanisms plant CTS...
Peroxidase-mimicking nanozymes offer unique advantages in terms of high stability and low cost over natural peroxidase for applications bioanalysis, biomedicine, the treatment pollution. However, design high-efficiency peroxidase-mimicking remains a great challenge. In this study, we adopted structural-design approach through hybridization cube-CeO2 Pt nanoparticles to create new nanozyme with efficiency excellent stability. Relative pure nanoparticles, as-hybridized Pt/cube-CeO2...
Species-specific enzyme detection refers to an important modality in the field of monitoring foodborne pathogens. Particularly, β-galactosidase (β-gal) has been extensively employed for ascertaining concentration Escherichia coli (E. coli). The major challenges facing recent works include improving accessibility, reducing analysis time, and increasing sensitivity techniques. In current study, a multicolor colorimetric platform involving enzyme–nanozyme cascade reaction-triggered etching gold...
A Cell Endocytosis-Activated Fluorescent (CEAF) probe triggered by disaggregation and protonation is designed for high contrast <italic>in vivo</italic> bioimaging diagnostics in the second near-infrared window (1000–1700 nm).
Alternaria is an endemic fungus associated with brown spot disease, which one of the most serious citrus diseases. In addition, mycotoxins metabolized by threaten human health seriously. Herein, a novel homogeneous and portable qualitative photothermal method based on recombinase polymerase amplification (RPA), CRISPR/Cas12a, rolling circle (RCA) for detection described. Using RCA primers as substrates CRISPR/Cas12a trans-cleavage, two systems, RPA-CRISPR/Cas12a RCA-enriched...
Abstract Selenium is an essential multifunctional trace element in diverse organisms. The only Se -glycosyltransferase identified that catalyzes the incorporation of selenium selenoneine biosynthesis SenB from Variovorax paradoxus . Although biochemical function has been investigated, its substrate specificity, structure, and catalytic mechanism have not elucidated. Here, we reveal exhibits sugar donor promiscuity can utilize six UDP-sugars to generate selenosugars. We report crystal...
We explore the interactions between a fluorescein (FAM)-labeled single-stranded DNA (P), graphene oxide (GO), and cationic conjugated polymer, poly [(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP). It is found that fluorescence change of P-GO-PFP system dependent on addition order P PFP. When adding PFP into P/GO complex, resonance energy transfer (FRET) from to inefficient. If added PFP/GO efficient FRET obtained. This may be attributed equal binding...
A fluorescence method for protein detection is developed based on terminal protection of small-molecule-linked DNA by target and a graphene oxide-assisted assay strategy. This design results in fluorescence-enhanced that sensitive selective the protein. As service to our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical support issues arising from...
The mechanism for short ssDNA having weaker affinity to graphene oxide than long was systematically investigated.
Clinical applications of current photodynamic therapy (PDT) agents are often restricted to be activated only by UV and visible light, which have very poor tissue penetration depths. In this study, a new near infrared (NIR)-absorbing nanoagent based on graphene oxide decorated with iron hydroxide/oxide (GO–FeOxH) was developed for light-activated nanomaterial-mediated PDT. This nanocomposite, GO–FeOxH prepared via the one-step electrooxidation nails in an aqueous GO solution. The as-prepared...
Glycosyltransferases are effective enzymes for glycosylating natural products (NPs), and some of them have the unusual property being exceedingly promiscuous catalytically toward a range substrates. UGT74AN3 is plant glycosyltransferase identified from Catharanthus roseus in our previous work. In this study, we found that exhibits high substrate promiscuity 78 acceptors 6 sugar donors also N-/S-glycosylation activity simple aromatic compounds. The crystal structures complex with various NPs...
Abstract A novel colorimetric pad with porous structure, good antimicrobial activity, and pH‐sensitivity has been developed based on polyvinyl alcohol (PVA), paper fibre, silver nanoparticles (AgNPs) purple sweet potato anthocyanins (PSPA), aiming for the preservation freshness indication of fresh meat. The effects AgNPs content physical properties colour‐changing behaviour in response to pH were investigated. results showed that physically interacted polymer matrix improved uniformity...