A5101612696- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Surface Modification and Superhydrophobicity
- Electrowetting and Microfluidic Technologies
- Cellular Mechanics and Interactions
- Electrohydrodynamics and Fluid Dynamics
- Streptococcal Infections and Treatments
- Biosensors and Analytical Detection
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Aerosol Filtration and Electrostatic Precipitation
- Extracellular vesicles in disease
- Cyclone Separators and Fluid Dynamics
- Antimicrobial Resistance in Staphylococcus
- Fungal Biology and Applications
- Bacterial Identification and Susceptibility Testing
- Hydraulic flow and structures
- Fluid Dynamics and Thin Films
- Inhalation and Respiratory Drug Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Sensor and Energy Harvesting Materials
University of Washington
2017-2025
Tasso (United States)
2017-2024
Seattle University
2017-2023
University of Idaho
2023
University of Wisconsin–Madison
2008-2020
University of Wisconsin Carbone Cancer Center
2013-2016
Madison Group (United States)
2015
PhylloTech (United States)
2014
Susquehanna University
2014
CEA Grenoble
2014
A microfluidic passive pumping method relying on surface tension properties is investigated and a physical model developed. When small inlet drop placed the entrance of channel it creates more pressure than large output at exit, causing fluid flow. The behavior input occurs in two characteristic phases. An analytical solution proposed verified by experimental results. We find that during first phase flow rate stable this can be prolonged refilling to produce continuous microchannel.
Although the field of microfluidics has made significant progress in bringing new tools to address biological questions, accessibility and adoption within life sciences are still limited. Open microfluidic systems have potential lower barriers adoption, but absence robust design rules hindered their use. Here, we present an open platform, suspended microfluidics, that uses surface tension fill maintain a fluid microscale structures devoid ceiling floor. We developed simple ubiquitous model...
Microfluidic cell-based systems have enabled the study of cellular phenomena with improved spatiotemporal control microenvironment and at increased throughput. While poly(dimethylsiloxane) (PDMS) has emerged as most popular material in microfluidics research, it specific limitations that prevent microfluidic platforms from achieving their full potential. We present here a complete process, ranging mold design to embossing bonding, describes fabrication polystyrene (PS) devices similar cost...
The utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work generally focused on the cells and, role fibroblast culture conditions tumor-stromal cell is still largely unknown. Here, we focus by comparing functional behaviors human mammary fibroblasts (HMFs) cultured 2D 3D their effects invasive progression breast (MCF10DCIS.com). We identified increased levels several paracrine factors...
Open microfluidic capillary systems are a rapidly evolving branch of microfluidics where fluids manipulated by forces in channels lacking physical walls on all sides. Typical channel geometries include grooves, rails, or beams and complex with multiple air–liquid interfaces. Removing allows access for retrieval (fluid sampling) addition (pipetting reagents adding objects like tissue scaffolds) at any point the channel; entire becomes "device-to-world" interface, whereas such interfaces...
Abstract The microbial secondary metabolome encompasses great synthetic diversity, empowering microbes to tune their chemical responses changing microenvironments. Traditional metabolomics methods are ill-equipped probe a wide variety of environments or environmental dynamics. Here we introduce class microscale culture platforms analyse diversity fungal and bacterial metabolomes. By leveraging stable biphasic interfaces integrate microculture with small molecule isolation via liquid–liquid...
We designed a device to investigate the limits of rapid injection molding for microscale features with cell culture applications.
Abstract We inhale respiratory pathogens continuously, and the subsequent signaling events between host microbe are complex, ultimately resulting in clearance of microbe, stable colonization host, or active disease. Traditional vitro methods ill-equipped to study these critical context lung microenvironment. Here we introduce a microscale organotypic model human bronchiole for studying pulmonary infection. By leveraging techniques, is designed approximate structure bronchiole, containing...
Background Efforts to minimize COVID-19 exposure during the current SARS-CoV-2 pandemic have led limitations in access medical care and testing. The Tasso-SST kit includes all of components necessary for remote, capillary blood self-collection. In this study, we sought investigate accuracy reliability device as a self-collection measurement IgG antibodies. Methods Capillary was obtained via unsupervised supervised application device, venous collected by standard venipuncture. Unsupervised...
Asthma is a chronic inflammatory disorder that affects more than 300 million people worldwide. management would benefit from additional tools establish biomarkers to identify phenotypes of asthma. We present microfluidic solution discriminates asthma allergic rhinitis based on patient's neutrophil chemotactic function. The handheld diagnostic device sorts neutrophils whole blood within 5 min, and generates gradient chemoattractant in the microchannels by placing lid with onto base device....
Abstract Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show an endogenously produced secreted oxylipin, 5,8-diHODE, induces cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus flavus , appressorium formation the rice blast pathogen Magnaporthe grisea . The response is specific to a subset of oxylipins...
Abstract Droplet microfluidics enables compartmentalized reactions in small scales and is utilized for a variety of applications across chemical analysis, material science, biology. While droplet successful technology, barriers include high “activation energy” to develop custom complex peripheral equipment. These limit the adoption labs or prototyping environments. This work demonstrates first time an open channel microfluidic system that autonomously generates droplets at low capillary...
Journal Article Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology proliferation of T47D breast carcinoma cells Get access Sara I. Montanez-Sauri, Montanez-Sauri Materials Science Program, University Wisconsin-Madison, Madison, WI, USAWisconsin Institutes for Medical Research, USAUniversity Wisconsin Carbone Cancer Center, USA Search other works by this author on: Oxford Academic Google Scholar Kyung Eun Sung, Sung USADepartment Biomedical...
The secondary metabolome provides pathogenic fungi with a plethoric and versatile panel of molecules that can be deployed during host ingress. While powerful genetic analytical chemistry methods have been developed to identify fungal metabolites (SMs), discovering the biological activity SMs remains an elusive yet critical task. Here, we describe process for identifying immunosuppressive properties Aspergillus by coupling cost-effective microfluidic neutrophil chemotaxis assay in vivo...
Microscale platforms are enabling for cell-based studies as they allow the recapitulation of physiological conditions such extracellular matrix (ECM) configurations and soluble factors interactions.
The hanging droplet technique for three-dimensional tissue culture has been used decades in biology labs, with the core technology remaining relatively unchanged. Recently microscale approaches have expanded capabilities of method, making it more user-friendly. We present a spontaneously driven, open platform to address many limitations current platforms. Our makes use two interconnected wells, larger well where cells are cultured and smaller user interface via pipette. two-well system...
We developed an open microfluidic hydrogel-patterning technology that creates networks of cell culture chambers in well plates for multiculture experiments.