A5000291310- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cardiac electrophysiology and arrhythmias
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Plant Virus Research Studies
- Molecular Biology Techniques and Applications
- Chromosomal and Genetic Variations
- Flowering Plant Growth and Cultivation
- Biosimilars and Bioanalytical Methods
- Acute Lymphoblastic Leukemia research
- Advanced Memory and Neural Computing
- Extracellular vesicles in disease
- Neurobiology and Insect Physiology Research
- Galectins and Cancer Biology
- stochastic dynamics and bifurcation
- Protein purification and stability
Northeast Agricultural University
2023-2025
Complete Genomics (United States)
2017-2024
BGI Group (China)
2016-2024
China National GeneBank
2019-2022
University of Chinese Academy of Sciences
2020-2021
Guangdong Academy of Agricultural Sciences
2020
Shenzhen Genoimmune Medical Institute
2017
Molecular Devices
2007
Jefferson College
1994-1996
Mutations in the skeletal muscle voltage-gated Na+ channel alpha-subunit have been found patients with two distinct hereditary disorders of sarcolemmal excitation: hyperkalemic periodic paralysis (HYPP) and paramyotonia congenita (PC). Six these mutations functionally expressed a heterologous cell line (tsA201 cells) using recombinant human cDNA hSkM1. PC mutants from diverse locations this subunit (T1313M, L1433R, R1448H, R1448C, A1156T) all exhibit similar disturbance inactivation...
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report compare cheaper BGISEQ-500 platform Illumina HiSeq for scRNA-seq. We generate a resource of 468 single cells and 1297 matched cDNA samples, performing SMARTer Smart-seq2 protocols on two cell lines with RNA spike-ins. sequence these libraries both platforms using single- paired-end reads. The have comparable sensitivity accuracy in terms quantification gene expression, low...
Predicting peptide binding affinity with human leukocyte antigen (HLA) is a crucial step in developing powerful antitumor vaccine for cancer immunotherapy. Currently available methods work quite well predicting HLA alleles such as HLA-A*0201, HLA-A*0101, and HLA-B*0702 terms of sensitivity specificity. However, few types that are present the majority populations including HLA-A*0202, HLA-A*0203, HLA-A*6802, HLA-B*5101, HLA-B*5301, HLA-B*5401, HLA-B*5701 still cannot be predicted satisfactory...
Compared to classical antibodies, camel heavy chain antibodies (HCAbs) are smaller in size due lack of the light and first constant domain (CH1 region). The variable regions HCAbs (VHHs) more soluble stable than that conventional (VHs). Even with such simple structure, they still functional antigen binding. Although have been extensively investigated over past two decades, most efforts based upon low throughput sequence analysis, there only limited reports trying analyze describe complete...
Chondrichthyan (cartilaginous fish) occupies a key phylogenetic position and is important for investigating evolutionary processes of vertebrates. However, limited whole genomes impede our in-depth knowledge issues such as chromosome evolution immunity. Here, we report the chromosome-level genome white-spotted bamboo shark. Combing it with other shark genomes, reconstructed 16 ancestral chromosomes illustrate dynamic rearrangement process. We found that genes on 13 fast-evolving can be...
SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading antibodies isolated earlier the pandemic. Investigation of memory B cells from patients primarily breakthrough infections Delta variant enables isolation a number neutralizing cross-reactive to heterologous concern (VOCs) including (BA.1-BA.4). Structural studies identify altered complementarity determining region (CDR) amino acids and unusual heavy chain CDR2 insertions respectively two...
<i>Xanthomonas euvesicatoria</i> has become a serious problem in <i>Physalis pubescens</i>, leading to substantial crop losses. In our previous investigation, we used rapid molecular detection techniques identify <i>X. euvesicatoria</i>; however, this pathogen’s diversity and population structure remain poorly understood, despite their importance disease management. To address knowledge gap, analyzed the of using BOX-PCR ERIC-PCR fingerprinting techniques....
The development of shark single domain antibodies (sdAbs) is hindered by the high cost and tediousness large-sized farming. Here, we demonstrated white-spotted bamboo sharks (Chiloscyllium plagiosum) being cultivated commercially as a promising small animal model to produce sdAbs. We found that immunoglobulin new antigen receptor (IgNAR) presented in genome, transcriptome, plasma. Four complete IgNAR clusters including variable domains (vNARs) were discovered germline, Variable-Joining pair...
T-type Ca2+ channels encoded by voltage-gated channel (Cav) 3.1, 3.2, and 3.3 genes play important physiological roles serve as therapeutic targets for neurological cardiovascular disorders. Currently there is no selective T-channel blocker. To screen such a blocker, we developed three stable cell lines expressing human recombinant Cav3.1, or then examined their usefulness in high throughput screens. All displayed an increase intracellular response to changes extracellular detected with...
In this study, the diversity of putative mycoviruses present in 66 strains binucleate Rhizoctonia (BNR, including anastomosis group (AG)-A, AG-Fa, AG-K, and AG-W) 192 multinucleate (MNR, AG-1-IA, AG-2-1, AG-3 PT, AG-4HGI, AG-4HGII, AG-4HGIII, AG-5), which are causal agents potato stem canker or black scurf, was studied using metatranscriptome sequencing. The number contigs related to identified from BNR MNR 173 485, respectively. On average, each strain accommodated 2.62 mycoviruses, while...
Abstract Background The advent of proprotein convertase subtilisin/kexin type 9 (PCSK9)–inhibiting drugs have provided an effective, but extremely expensive treatment for the management low density lipoprotein (LDL). Our aim was to explore a cost‐effective application camelid anti‐PCSK9 single domain antibodies (sdAbs), which are high variable regions heavy chain (VHHs), as human PCSK9 (hPCSK9) inhibitor. One female llama immunized with hPCSK9. Screening affinity VHHs carried out based on...
SUMMARY In response to antigens, B cells undergo affinity maturation and class switching mediated by activation-induced cytidine deaminase (AID) in germinal centers (GCs) of secondary lymphoid organs, but uncontrolled AID activity can precipitate autoimmunity cancer. The regulation GC antibody diversification is fundamental importance not well understood. We found that autoimmune regulator (AIRE), the molecule essential for T cell tolerance, expressed a CD40-dependent manner, interacts with...
The 0.8-Mb Ig new Ag receptor (IgNAR) region of the whitespotted bamboo shark (Chiloscyllium plagiosum) is incompletely assembled in Chr_44 reference genome. Here we used Cas9-assisted targeting chromosome segments (CATCH) to enrich 2 Mb IgNAR loci and sequenced it by PacBio next-generation sequencing. A fragment >3.13 was isolated intact from RBCs sharks. target enriched 245.531-fold, sequences had up 94% coverage with a 255× mean depth. Compared previously published sequences, 20 holes...
Abstract Background/Purpose: Chimeric antigen receptor (CAR) T cell therapy has shown remarkable responses in hematologic malignancies. However, suboptimal clinical outcomes have been associated with decreased expansion and persistence of adoptive transferred CAR-T cells, antigen-negative relapses, impairment by an immunosuppressive tumor microenvironment. We previously developed CD19CAR-T cells secreting PD-1Ab21 fusion protein composed anti-PD-1 single chain antibody...
Sauropus spatulifolius Beille is one kind of Chinese herbal medicine with anti-inflammatory and analgesic activities. In this study, we reported the complete chloroplast genome S. Beille, assembled annotated high-throughput sequencing data, which would provide help for its taxonomy research. The sequence was 154,707 bp , two 87,438 19,427 single-copy regions, were separated by inverted repeat regions 23,921 . A total 129 genes predicted, GC content 36.61%. Phylogenetic analysis showed that...
In this report, one novel method has been developed to screen the monoclonal antibody against human pancreatic cancer biomarker glypican-1 (GPC1) through combination of fluorescent cell sorting and single B amplification. GPC1-positive cells were sorted out from peripheral blood mononuclear (PBMCs) by after GPC1 immunization New Zealand white rabbit. Then, total RNA was extracted reversely transcribed into cDNA, which used as template, variable region sequences both heavy light chains...
Abstract PCSK9 is an effective target for lowering LDL-c. Previously, a camelid-human chimeric heavy chain antibody VHH-B11-Fc targeting human was designed. It had potent hypolipidemic effect. However, the nanobody VHH-B11 interacts with at low affinity, while camelid VHH exhibits some immunogenicity. Moreover, interacting epitope yet to be identified, although shown have distinct hPCSK9-binding epitopes Evolocumab. This might impede molecule’s progress from bench bedside. In present study,...
Abstract Cartilaginous fishes have a very high phenotypical diversity, phenomenon for which the mechanisms been largely unexplored. Here, we report genome of white-spotted bamboo shark as first chromosome-level assembly cartilaginous fish. Using this genome, illustrated dynamic chromosome rearrangement process in shark, resulted formation 13 chromosomes, all were sparsely distributed with conserved genes and fast-evolving. We found fast-evolving chromosomes to be enriched immune-related two...