A5101553052- Immune Cell Function and Interaction
- interferon and immune responses
- Dialysis and Renal Disease Management
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Cellular transport and secretion
- Restless Legs Syndrome Research
- Acute Myeloid Leukemia Research
- Atherosclerosis and Cardiovascular Diseases
- Chronic Kidney Disease and Diabetes
- Plant and Fungal Species Descriptions
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Genomics, phytochemicals, and oxidative stress
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Forensic Toxicology and Drug Analysis
- Acute Lymphoblastic Leukemia research
- Endoplasmic Reticulum Stress and Disease
- Sleep and related disorders
- CAR-T cell therapy research
- Identification and Quantification in Food
Guangdong Academy of Agricultural Sciences
2020-2024
Tianjin First Center Hospital
2021-2024
Tianjin Medical University
2021-2024
Second Hospital of Tianjin Medical University
2022
West China Medical Center of Sichuan University
2021-2022
Second Military Medical University
2009-2021
Pudong Medical Center
2016-2021
Fudan University
2015-2021
University of Sussex
2019-2020
Xian Yang Central Hospital
2019
Abstract N6-methyladenosine (m 6 A) modification plays important roles in various cellular responses by regulating mRNA biology. However, how m A is involved innate immunity via affecting the translation of immune transcripts remains to be further investigated. Here we report that RNA methyltransferase Mettl3-mediated methylation promotes dendritic cell (DC) activation and function. Specific depletion Mettl3 DC resulted impaired phenotypic functional maturation DC, with decreased expression...
Two mitotic cyclin types, A and B, exist in higher eukaryotes, but their specialised functions mitosis are incompletely understood.Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis.Loss G2-phase prevents entry.Cells lacking B can enter phosphorylate most proteins, because parallel PP2A:B55 phosphatase inactivation by Greatwall kinase.The final barrier to establishment corresponds nuclear envelope breakdown, which requires...
Abstract Regulatory T (Treg) cells are important for the maintenance of immune homoeostasis and prevention autoimmune diseases. Epigenetic modifications have been reported to modulate autoimmunity by altering Treg cell fate. Here we show that H3K4 methyltransferase Ash1l facilitates TGF-β-induced polarization in vitro protects mice from cell-mediated colitis vivo . upregulates Smad3 expression directly targeting promoter increase local trimethylation. Furthermore, identify an lncRNA, namely...
Cdc42-associated tyrosine kinase 1 (ACK1) is a specific down-stream effector of Cdc42, Rho family small G-protein. Previous studies have shown that ACK1 interacts with clathrin heavy chain and involved in clathrin-coated vesicle endocytosis. Here we report interacted epidermal growth factor receptor (EGFR) upon EGF stimulation via region at carboxy terminus highly homologous to Gene-33/Mig-6/RALT. The interaction EGFR was dependent on the activity or phosphorylation EGFR. Immunofluorescent...
Protein ubiquitination regulated by ubiquitin ligases plays important roles in innate immunity. However, key regulators of during response and new types (apart from Lys48- Lys63-linkage) control signaling have not been clearly understood. Here we report that F-box only protein Fbxo21, a functionally unknown component SCF (Skp1–Cul1–F-box protein) complex, facilitates Lys29-linkage activation ASK1 (apoptosis signal-regulating kinase 1), promotes type I interferon production upon viral...
To explore whether epigallocatechin-3-gallate (EGCG) improves renal damage in diabetic db/db mice and high-glucose- (HG-) induced injury HK-2 cells by regulating the level of Klotho gene promoter methylation. Western blotting was used to detect protein expression levels DNA methyltransferase 1 (DNMT1), DNMT3a, DNMT3b, transforming growth factor- β (TGF- 1), α -smooth muscle actin ( -SMA), Klotho. The methylation detected pyrosequencing. Chromatin immunoprecipitation binding DNMT1 DNMT3a....
Abstract Morinda officinalis is a well-known medicinal and edible plant that widely cultivated in the Lingnan region of southern China. Its dried roots (called bajitian traditional Chinese medicine) are broadly used to treat various diseases, such as impotence rheumatism. Here, we report high-quality chromosome-scale genome assembly M. using Nanopore single-molecule sequencing Hi-C technology. The assembled size was 484.85 Mb with scaffold N50 40.97 Mb, 90.77% sequences were anchored on...
Abstract Background Maternal air pollutants exposure is associated with a number of adverse pregnancy outcomes, including recurrent spontaneous abortion (RSA). However, the underlying mechanisms are still unknown. The present study aimed to understand mechanism RSA and its relationship pollution exposure. We compared data decidual tissue from individuals induced abortions those by bulk RNA sequencing (RNA-seq), reduced representation bisulfite (RRBS), single-cell (scRNA-seq). Differentially...
CYP6F1 (GenBank/EMBL accession No. AY662654), a novel gene with complete encoding sequence in the cytochrome P450 family 6, was cloned and sequenced from deltamethrin-resistant 4th instar larvae of Culex pipiens pallens. The cDNA has an open reading frame 1527 bp, which encodes putative protein 508 amino acid residues. deduced indicated that encoded conserved domains membrane-anchoring signal, reductase-binding sites, typical heme-binding site, ETLR motif substrate recognition sites....
Sensing of pathogenic nucleic acids by pattern recognition receptors (PRR) not only initiates anti-microbe defense but causes inflammatory and autoimmune diseases. E3 ubiquitin ligase(s) critical in innate response need to be further identified. Here we report that the tripartite motif-containing ligase TRIM41 is required antiviral through facilitating acids-triggered signaling pathway. deficiency impairs production cytokines type I interferons macrophages after transfection with acid-mimics...
Abstract The β2 integrins play a key role in inflammation and immune responses. integrin CD11b has been shown recently to be important the maintenance of tolerance; however, underlying mechanisms remain fully understood. Natural killer (NK) cells are an effector innate immunity but also regulator adaptive response. How activating inhibitory signals balanced determine NK cell function needs further identified. expression was dramatically up-regulated on once they matured became activated;...
Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: expression significantly reduced subsets ALL patients, low correlates with cell proliferation an elevation several poor prognostic markers B-cell ALL. directly...
Erythropoietin (EPO) can reduce insulin resistance (IR) in adipocytes; however, it is unknown whether EPO decrease IR skeletal muscle.Here we investigated could type 2 diabetic mouse muscle and its possible signaling mechanisms of action.Twelve-week-old db/db mice were employed this study.Systemic use improved glucose profiles after 4 8 weeks treatment.EPO up-regulated EPOR protein expression muscle, subsequently activated downstream molecules such as JAK2, IRS-1, PI3K, AKT, eNOS.We next...
SUMMARY The Phyllanthaceae family comprises a diverse range of plants with medicinal, edible, and ornamental value, extensively cultivated worldwide. Polyploid species commonly occur in Phyllanthaceae. Due to the rather complex genomes evolutionary histories, their speciation process has been still lacking research. In this study, we generated chromosome‐scale haplotype‐resolved two octoploid ( Phyllanthus emblica Sauropus spatulifolius ) family. Combined our previously reported one...
Patients with maintenance hemodialysis have experienced long-standing sleep disturbance. In this study, we attempted to explore whether long-term hemoperfusion could improve and increase the overall survival in patients.A total of 158 patients, who underwent routine hemodialysis, were assessed study. These patients computer-matched into two groups, one group including 80 absolute other consisting 78 cases combination hemoperfusion. Hemoperfusion was performed 1-2 times biweekly, each session...
Research suggests that fine particulate matter (PM2.5) contributes to the expansion and development of atherosclerosis. Infiltration proliferation vascular smooth muscle cells (VSMCs) from blood vessel media into intima, is an important step in atherosclerosis pathophysiology. Afrocyclamin A, oleanane-type triterpene saponin, isolated