A5016841417- interferon and immune responses
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- Extracellular vesicles in disease
- Heat shock proteins research
- Melanoma and MAPK Pathways
- Retinoids in leukemia and cellular processes
- RNA Research and Splicing
- Cancer-related Molecular Pathways
Second Military Medical University
2009-2022
Institute of Immunology
2006-2022
University Medical Center Freiburg
2014
Chinese Academy of Medical Sciences & Peking Union Medical College
2014
Zhejiang University
2003-2006
Abstract Extracellular heat shock proteins (HSP) can activate dendritic cells (DC) and monocytes/macrophages, HSP derived from tumor have been regarded as potent adjuvant facilitating presentation of Ags induction antitumor immunity. However, the roles underlying mechanisms releasable in immunity not fully elucidated. In this study, we report that stress induce release various cells, which, turn, to produce chemokines for chemoattraction DC T via TLR4 signaling pathway. vivo, find...
Exosomes derived from dendritic cells or tumor are a population of nanometer-sized membrane vesicles that can induce specific antitumor immunity. During investigation the effects hyperthermia on immune response, we found exosomes heat-stressed (HS-TEX) could chemoattract and activate (DC) T more potently than by conventional tumor-derived exosomes. We show HS-TEX contain chemokines, such as CCL2, CCL3, CCL4, CCL5, CCL20, chemokine-containing functionally competent in chemoattracting CD11c(+)...
Abstract Purpose: Tumor-derived exosomes are proposed as a new type of cancer vaccine. Heat shock proteins potent Th1 adjuvant, and heat stress can induce protein MHC-I expression in tumor cells, leading to the increased immunogenicity cells. To improve vaccine, we prepared from heat-stressed carcinoembryonic antigen (CEA)–positive cells (CEA+/HS-Exo) tested efficacy these induction CEA-specific antitumor immunity. Experimental Design: First, identified composition CEA+/HS-Exo observed their...
Promoting apoptosis is a strategy for cancer drug discovery. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces in wide range of malignant cells. However, several cancers, including human hepatocellular carcinoma (HCC), exhibit major resistance to TRAIL-induced cell death. Melittin, water-soluble 26-amino acid peptide derived from bee venom Apis mellifera, can exert toxic or inhibitory effects on many types tumor Here we report that melittin induce HCC cells by...
RNA-binding proteins (RBPs) play important roles in cancer development and treatment. However, the tumor-promoting RBPs their partners, which may potentially serve as therapeutic targets, need to be further identified. Here, we report that zinc finger CCHC domain-containing protein 4 (ZCCHC4) is of aberrantly high expression multiple human tissues associated with poor prognosis chemoresistance patients hepatocellular carcinoma (HCC), pancreatic colon cancer. ZCCHC4 promotes HCC cells...
The carboxyl terminus of constitutive heat shock cognate 70 (HSC70)-interacting protein (CHIP, also known as Stub1) is a U box-containing E3 ubiquitin ligase that important for quality control. role CHIP in innate immunity not known. Here, we report knockdown inhibits Toll-like receptor (TLR) 4- and TLR9-driven signaling, but TLR3-driven signaling; proinflammatory cytokine type 1 interferon (IFN) production; maturation antigen-presenting cells, including macrophages dendritic cells. We...
microRNA (miRNA) dysregulation is involved in the development and progression of various human cancers, including hepatocellular carcinoma (HCC). However, how to identify miRNAs targeting specific mRNA cells a significant challenge because interaction complexity limited knowledge rules governing these processes. Some are not predictable by current computer algorithms available. Here, using p21 as target, we established new method, called miRNA vivo precipitation (miRIP), which kind can...
Pathological retinal angiogenesis resulting from a variety of ocular diseases including oxygen induced retinopathy, diabetic retinopathy and vein occlusion, is one the major reasons for vision loss, yet therapeutic option limited. Multiple nanoparticles have been reported to alleviate angiogenic retinopathy. However, adverse effect cannot be ignored due relatively large scale. Graphene quantum dots (GQDs) shown potential in drug delivery proved biocompatible. In this study, are extensively...
The endoplasmic reticulum (ER)–localized stimulator of interferon genes (STING) is the core adaptor for pathogenic-DNA–triggered innate response. Aberrant activation STING causes autoinflammatory and autoimmune diseases, raising concern about how finely tuned during response to pathogenic DNAs. Here, we report that transmembrane domain (TM)–containing ER-localized E3 ubiquitin ligase TRIM13 (tripartite motif containing 13) required restraining inflammatory deficiency enhances cytokine...
Protein ubiquitination regulated by ubiquitin ligases plays important roles in innate immunity. However, key regulators of during response and new types (apart from Lys48- Lys63-linkage) control signaling have not been clearly understood. Here we report that F-box only protein Fbxo21, a functionally unknown component SCF (Skp1–Cul1–F-box protein) complex, facilitates Lys29-linkage activation ASK1 (apoptosis signal-regulating kinase 1), promotes type I interferon production upon viral...
Various pattern recognition receptors (PRRs) are activated in response to viral infection stimulate the production of type I interferons (IFNs). However, central responses all these is their activation kinase TBK1, which stimulates transcription by IFN regulatory factor 3 (IRF3). We investigated mechanism activity TBK1 stimulated infection. found that tyrosine Src promoted phosphorylation on Tyr179 upon RAW264.7 macrophages. Mutation alanine resulted impaired autophosphorylation at Ser172,...
Abstract Chemokines play important roles in leukocyte trafficking as well function regulation. In this study, we described the identification and characterization of a novel CXC chemokine from human dendritic cell (DC) cDNA library, full-length which contains an open reading frame encoding 111 aa with putative signal peptide 34 aa. This shares greatest homology macrophage inflammatory protein (MIP)-2αβ, hence is designated MIP-2γ. Mouse MIP-2γ was identified by electrocloning highly...
The Siglecs (sialic acid-binding Ig-like lectins) are a subfamily of I-type lectins, which specifically recognize sialic acids. Nine members the family have been identified thus far. We obtained novel cDNA clone from human dendritic cell library encoding protein with sequence and structural features Siglec family, hence designated as Siglec-10. full-length Siglec-10 encodes type 1 transmembrane containing four extracellular immunoglobulin-like domains, region, cytoplasmic tail two classical...
Lysosomes have recently been identified as important apoptotic signal integrators in response to various stimuli. Here we report the functional characterization of LAPF, a novel lysosome-associated apoptosis-inducing protein containing PH and FYVE domains. LAPF is representative new family, Phafins (protein both domains), which consists 14 unidentified proteins from species. Overexpression L929 cells induces apoptosis also increases cell sensitivity TNFα-induced apoptosis, concomitant with...
Inappropriate activation of TLR9 has been found to be involved in the pathogenesis autoimmune diseases such as systemic lupus erythematosus. antagonists have proposed therapeutic for some kinds diseases. In contrast, new negative regulators signal pathway need identified, and mechanisms control response fully investigated. It is well known that will finally transported late endosome/lysosome once activated; however, exact mechanism biological significance redistribution not elucidated. Ras...