Background: The aim of this study was to characterize the disorder lipid metabolism in hepatocellular carcinoma (HCC). HCC is a worldwide disease. research into very limited. Study liver cancer tissue may have potential provide new insight mechanisms. Methods: A lipidomics based on Ultra high performance liquid chromatography-electronic spray ionization-QTOF mass spectrometer (UPLC-ESI-QTOF MS) and Matrix assisted laser desorption ionization-fourier transform ion cyclotron resonance...

Pathological retinal angiogenesis resulting from a variety of ocular diseases including oxygen induced retinopathy, diabetic retinopathy and vein occlusion, is one the major reasons for vision loss, yet therapeutic option limited. Multiple nanoparticles have been reported to alleviate angiogenic retinopathy. However, adverse effect cannot be ignored due relatively large scale. Graphene quantum dots (GQDs) shown potential in drug delivery proved biocompatible. In this study, are extensively...

To investigate the value of chaperonin containing TCP1, subunit 3 (CCT3) to predict prognosis patients with hepatocellular carcinoma (HCC) and determine its function in HCC progression.CCT3 expression levels were examined human non-cancerous liver tissues a variety cell lines by quantitative real-time PCR immunoblotting. CCT3 was suppressed small interfering RNA. The effects reducing cells tested. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay, counting experiment,...

Tumor heterogeneity dominates tumor biological behavior and shapes the microenvironment. However, mechanisms of genetic features modulate immunity response were not clearly clarified. associated macrophages (TAMs) exert distinct immune functions in progression hepatocellular carcinoma (HCC) based on inducible phenotype. FOXO family members sense changes extracellular or intracellular environment by activating a series signaling pathways. FOXO1, transcription factor that common suppressor...

Black phosphorus quantum dots (BPQDs) have shown potential in tumor therapy, however, their anti-angiogenic functions not been studied. Although BPQDs are easily degraded to non-toxic phosphrous, the reported toxicity, poor stability, and non-selectivity largely limit further application medicine. In this study, a vascular targeting, biocompatible, cell metabolism-disrupting nanoplatform is engineered by incorporating into exosomes modified with Arg-Gly-Asp (RGD) peptide (BPQDs@RGD-EXO...

Abstract Interferon regulatory factor 1 (IRF1) has been found to serve as a tumor suppressor in cholangiocarcinoma, and enabled prediction of clinical progression prognosis our previous study. The objective the current study is screen identify valuable microRNAs (miR), which target IRF1 regulate cholangiocarcinoma cell proliferation, migration, invasion. High expression miR‐383 was observed tissues cells. Meanwhile, we predicted binding site on 3′‐untranslated region (3′‐UTR) according miR...

Organ-like microenviroment and 3-dimensional (3D) cell culture conformations have been suggested as promising approaches to mimic in a micro-scale whole organ cellular functions interactions present vivo. We used this approach examine biologic features of hepatocellular carcinoma (HCC) cells. In study, we demonstrate that cells, fibroblasts, endothelial cells extracellular matrix can generate organoid-like spheroids enhanced numerous human HCC observed show the addition non-parenchymal such...

Scalp acupuncture has shown a remarkable treatment efficacy on motor dysfunction in patients with stroke China, especially the area of Jiao's scalp acupuncture, which is most widely used treatment. However, previous studies have summarized that clinical curative effect for remains uncertain. Meanwhile, no randomized controlled trials been performed. The aim this study to evaluate and safety ischemic stroke. This an assessor- analyst-blinded, trial. One hundred eight meeting inclusion...

CHK1 is considered an oncogene with overexpression in numerous cancers. However, signalling regulation hepatocellular carcinoma (HCC) remains unclear. CHEK1 mRNA, protein, pri-miR-195 and miR-195 expression HCC tissue was determined by qPCR, WB IF staining assay. Survival analyses high- low-CHEK1 mRNA performed using TCGA database. Relative luciferase activity investigated cells transfected p-CHEK1 3'UTR. Apoptosis detected TUNEL NK CD8+ T were analysed flow cytometry. increased human...

Abstract Background Cancer development is strictly correlated to composition and physical properties of the extracellular matrix. Particularly, a higher matrix stiffness has been demonstrated promote tumor sustained growth. Our purpose was explore role in liver cancer development. Methods The tissues determined by atomic force microscopy (AFM) analysis. In vitro, we used tunable Polyacrylamide (PA) hydrogels culture system for cells culture. expression level integrin β1, phosphorylated FAK,...

Inflammation is a core mechanism for oncogenesis. Chemokines act as important mediators of chronic inflammation and the tumour inflammatory response. However, there limited information on chemokines in hepatocellular carcinoma (HCC), disease which almost all cases are derived from liver inflammation. Here, we explored protumor effects CXCL1, commonly elevated chemokine cirrhosis, HCC. The role was confirmed clinical samples HCC patients. CXCL1 enhanced tumorigenesis hepatic microenvironment...

Abstract Metastasis is the major cause of cancer-related death in patients with colorectal cancer. Although inducible nitric oxide synthase (iNOS) a crucial regulator cancer development and progression, its roles epithelial–mesenchymal transition (EMT) pathogenesis metastatic have not been fully investigated. Primary liver tissue specimens were analyzed showing 90% reduced expressions iNOS compared 6% primary The Cancer Genome Atlas database analyses via cBioPortal reveal that mRNA...

Background: Scalp acupuncture has been found to be effective at improving motor function after ischemic stroke, but few studies examining its central mechanisms of action have carried out. The aim this study was investigate the clinical effects scalp on dysfunction and changes in spontaneous brain activity patients with stroke. Methods: This an evaluator- analyst-blinded, multi-center randomized controlled trial. A total 108 convalescent-stage stroke were allocated receive either combined...

Objective: To evaluate the effects of community-based rehabilitation therapy on neurological function deficit in stroke patients. Design: Prospective, single-blind, randomized controlled multicentre trial. Setting: At home, Shanghai, China. Subjects: A total 737 patients community. Intervention: The group received additional standardized at home for five months. Main outcome measures: Patients were evaluated using Clinical Neurological Function Deficit Scale before intervention and end two...

OBJECTIVE: Previous studies have indicated that diffusion tensor imaging (DTI) values are related to clinical outcome in stroke patients. This prospective study explored whether DTI were predictive for hand function chronic METHODS: The parameters (rλ 1 , rλ 23 fractional anisotropy [rFA] and mean diffusivity [rMD]) investigated patients with completely paralysed hands (CPH; n = 10) or partially (PPH; 10), by two methods of analysis: segment the corticospinal tract [sCST] analysis; pure...

Abstract As a major cause of blindness, diabetic retinopathy (DR) is often found in the developed countries. Our previous study identified down-regulated miRNA: miR-144-3p response to hyperglycemia. The present aims investigate role proliferation microvascular epithelial cells. Endothelial cells were treated with different concentrations glucose, after which detected real-time PCR assay. MiR-144-3p mimics or inhibitors used increase knockdown level this miRNA. Western blotting assay and...

Breast cancer is one of the most common cancers worldwide, posing a serious threat to human health. Recently, innate immunity has become widely discussed topic in antitumor research. The STING pathway an important component immunity, and several agonists have been developed applied Dimeric amidobenzimidazole (diABZI) agonist nucleotide analog with low serological stability cell membrane permeability. In this study, we prepared diABZI-encapsulated liposomes (dLNPs) using ammonium sulfate...

Transforming growth factor beta 1 (TGFβ1) is a pleiotropic cytokine in the skin that can function both as tumor promoter and suppressor chemically induced carcinogenesis, but ultraviolet B (UVB) carcinogenesis not well understood. Treatment of SKH1 hairless mice with activin-like kinase 5 (ALK5) inhibitor SB431542 to block UVB-induced activation cutaneous TGFβ1 signaling suppressed formation did alter size or cell proliferation. Tumors arose SB-treated after 30 weeks had significantly...

As a combination of visual stimulation and motor imagery, mirror feedback (MVF) is an effective treatment for impairment after stroke; however, few studies have investigated its effects on relevant cognitive processes such as perception imagery. Camera-based MVF (camMVF) overcomes the intrinsic limitations real mirrors recognised optimal setup. This study aims to investigate camMVF adjunct stroke patients, compare outcomes with those conventional therapy elucidate neural mechanisms through...

Purpose: Mirror therapy (MT) is an effective adjunct treatment for Bell's palsy (BP); however, a bifold mirror-based apparatus hindered the effectiveness. Besides, few studies have reported related factors of facial embodiment. The aim this study was to evaluate feasibility novel face mirroring System (FMS) and effects on embodiment in BP patients, comparing with conventional mirror book (MB) therapy.Method: This within-subject design trial. Thirty-six patients were recruited received...

To study the effect of discoidin I-like domaincontaining protein 3 (EDIL3) depletion on proliferation and epithelial-mesenchymal transition (EMT) in human lens epithelial cells (LECs).RNA interference was used to inhibit expression EDIL3 LECs vitro. The morphology observed using an inverted microscope. Cell assessed EdU kit. migration investigated Transwell chamber EMT confocal microscope Western blotting. transforming growth factor β (TGFβ) pathway blotting.The data showed that silencing...

Orthotopic liver transplantation is currently the best treatment option for selected patients with hepatocellular carcinoma (HCC). From 1980 to 2011, 8874 HCC in China underwent transplantation. The organ donation classification criteria of (China criteria), which are established by Government China, divided into three parts: I, after brain death; II, cardiac death and III, dual brain-cardiac death. Data from Liver Transplant Registry(CLTR) System shows that within Milan have higher survival...

To evaluate the effect of individualized scalp acupuncture base on location brain function for motor dysfunction in stroke patients.A total 180 patients were randomly assigned into an (ISA) group, a conventional (CSA) group and rehabilitation 60 cases each one. In ISA we stimulated Sishencong (EX-HN 1), area balance area, matched with pre-motor higher muscle tension, application NIE 's three-needle involuntary motion, poor coordination, forehead five-needle cognitive disorder, sensory...