A5053420449- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
- Lipid metabolism and biosynthesis
- Metal complexes synthesis and properties
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
- Bipolar Disorder and Treatment
- Diet and metabolism studies
- Advanced biosensing and bioanalysis techniques
- Eicosanoids and Hypertension Pharmacology
- Mercury impact and mitigation studies
- Synthesis and Characterization of Heterocyclic Compounds
- Peroxisome Proliferator-Activated Receptors
- Diet, Metabolism, and Disease
- Circular RNAs in diseases
- Hemoglobin structure and function
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- Mental Health Research Topics
- Parasites and Host Interactions
- Quinazolinone synthesis and applications
- Cancer Mechanisms and Therapy
- Ion-surface interactions and analysis
National Taipei University of Nursing and Health Science
2025
Chinese Academy of Sciences
2013-2022
Tongji University
2013-2022
Fujian Normal University
2022
Ningbo Institute of Industrial Technology
2022
Beijing National Laboratory for Molecular Sciences
2013-2021
Center for Excellence in Education
2020-2021
Yuhuangding Hospital
2021
Qingdao University
2021
Fifth Affiliated Hospital of Sun Yat-sen University
2021
Background: Stroke is a leading cause of adult disability that can severely compromise the quality life patients, yet no effective medication currently exists to accelerate rehabilitation. A variety circular RNA (circRNA) molecules are known function in ischemic brain injury. Lentivirus-based expression systems have been widely used basic studies circRNAs, but safety issues with such delivery limited exploration potential therapeutic roles for circRNAs. Methods: Circular SCMH1 (circSCMH1)...
Stroke is a leading cause of death and disability but most the clinical trials have failed in past, partially because majority current approaches are focusing on neural protection rather than neuroregeneration. In this study, we report an vivo regeneration approach through AAV NeuroD1-based gene therapy to repair damaged brains after ischemic stroke adult non-human primates (NHPs). We demonstrate that ectopic expression transcription factor NeuroD1 reactive astrocytes monkey cortical can...
In contrast to the wide spectrum of cytochrome P450 monooxygenases, there are only 2 heme-based dioxygenases in humans: tryptophan dioxygenase (hTDO) and indoleamine 2,3-dioxygenase (hIDO). hTDO hIDO catalyze same oxidative ring cleavage reaction L-tryptophan N-formyl kynurenine, initial rate-limiting step kynurenine pathway. Despite immense interest, mechanism by which enzymes execute remains elusive. Here, we report experimental evidence for a key ferryl intermediate that supports atoms...
The recent escalation of US military suicide deaths to record numbers has been a sentinel for impaired force efficacy and accelerated the search reversible risk factors.To determine whether deficiencies neuroactive, highly unsaturated omega-3 essential fatty acids (n-3 HUFAs), in particular docosahexaenoic acid (DHA), are associated with increased death among large random sample active-duty military.In this retrospective case-control study, serum were quantified as percentage total (n = 800)...
Dietary intake of linoleic acid (LNA, 18:2n-6) has increased dramatically during the 20th century and is associated with greater prevalence obesity. The endocannabinoid system involved in regulation energy balance a sustained hyperactivity may contribute to Arachidonic (ARA, 20:4n-6) precursor for 2-AG anandamide (AEA), we sought determine if low fat diets (LFD) could be made obesogenic by increasing pool ARA, causing excessive signaling leading weight gain metabolic profile Mice (C57BL/6j,...
Background: The aim of this study was to characterize the disorder lipid metabolism in hepatocellular carcinoma (HCC). HCC is a worldwide disease. research into very limited. Study liver cancer tissue may have potential provide new insight mechanisms. Methods: A lipidomics based on Ultra high performance liquid chromatography-electronic spray ionization-QTOF mass spectrometer (UPLC-ESI-QTOF MS) and Matrix assisted laser desorption ionization-fourier transform ion cyclotron resonance...
Dietary intake of linoleic acid (LA) has increased dramatically during the twentieth century and is associated with a greater prevalence obesity. Vegetable oils are recognised as suitable alternatives to fish oil (FO) in feed for Atlantic salmon (Salmo salar L.) but introduce high amounts LA fillet. The effect on consumers such replacement remains be elucidated. Here, we investigate excessive dietary from soyabean (SO) endocannabinoid levels mice, study metabolic effects mice when SO...
Abstract MALDI MSI has been recently applied as an innovative tool for detection of molecular distribution within a specific tissue. requires deposition organic compound, known matrix, on the tissue interest to assist analyte desorption and ionization, in which matrix crystal homogeneity size greatly influence imaging reproducibility spatial resolution MSI. In this work, homemade electrospray device was developed The could be used achieve 1 μm homogeneous crystals analysis. Moreover, it...
Numerous studies have demonstrated the apoptotic and anti‑proliferative effects of resveratrol, a natural polyphenolic phytoalexin, on various cancer cell lines. However, resveratrol regulation human cervical carcinoma, mechanisms underlying these effects, remain to be elucidated. In present study, potential in HeLa carcinoma cells were investigated. The results revealed that inhibited proliferation induced apoptosis dose‑dependent time‑dependent manner. Resveratrol shrinkage accompanied by...
Human indoleamine 2,3-dioxygenase (hIDO) is an intracellular heme-containing enzyme, which catalyzes the initial and rate-determining step of L-tryptophan (L-Trp) metabolism via kynurenine pathway. Due to its immunosuppressive function, hIDO has been recognized as important drug target for cancer. Here we report evidence supporting presence inhibitory substrate binding site (S(i)) in that capable molecules with a wide variety structures, including substrates (L-Trp 1-methyl-L-tryptophan),...
Little is known about the uptake or metabolism of essential fatty acids (EFAs) in various mammalian organs. Thus, distribution deuterated alpha-linolenic acid (18:3n-3) and linoleic (18:2n-6) their metabolites was studied using a stable isotope tracer technique. Rats were orally administered single dose mixture (20 mg each) ethyl D5-18:3n-3 D5-18:2n-6, 25 tissues per animal analyzed for D5-labeled PUFAs at 4, 8, 24, 96, 168, 240, 360, 600 h after dosing. Plasma, stomach, spleen contained...
Increasing macroalgal blooms as a consequence of climate warming and coastal eutrophication have profound effects on the marine environment. The outbreaks Ulva prolifera in Yellow Sea China occurring every summer since 2007 to present formed world's largest green tide. tide releases huge amounts dissolved organic matter (DOM) seawater, causing an overload. However, how bacteria respond this issue potential impact environment are still unclear. Here, we monitored highly temporally resolved...
Ruthenium-based anticancer complexes are promising antitumor agents for their low system toxicity and versatile chemical structures. Epidermal growth factor receptor (EGFR) has been found to be overexpressed in a broad range of tumor cells is regarded as drug target developing novel drugs. In this work, five ruthenium(II) polypyridyl containing EGFR-inhibiting 4-anilinoquinazoline pharmacophores were synthesized characterized. These showed both high activity strong DNA minor groove-binding...
Cyclometallated platinum complexes bearing 4-anilinoquinazolines exhibit high potential as luminescent probes for EGFR/DNA in living cells and dual-targeting anticancer agents.
Abstract Aim This study evaluated the effect of cooperative learning on attitude, satisfaction, and performance undergraduate nursing students enrolled in community courses. Design A one-group pretest–posttest quasi-experimental design. Methods was conducted at a university northern Taiwan. total 49 fourth-year were included study. The students’ attitude toward measured using Chinese version Group Cooperative Learning scale three time points: before intervention, after 8-week preclinical...
Background— Apoptosis repressor with caspase recruitment domain (ARC) is abundantly expressed in cardiomyocytes. Protein kinase CK2 can phosphorylate ARC at threonine-149, thereby enabling to antagonize apoptosis. phosphorylation occurs a constitutive manner. Nevertheless, cardiomyocytes still undergo apoptosis that related cardiac diseases such as myocardial infarction and heart failure. Whether the occurrence of loss protection by under pathological conditions remains unknown. Methods...
Large population studies show that polyunsaturated fatty acids are important for human health, but determining relationships between the health benefits and acid content has been hampered by unavailability of labor-effective high-throughput technologies. An automated high throughput analysis was developed from a previous procedure based on direct transesterification including automation chemical procedures, data acquisition automatic processing. The method validated applied to umbilical cord...
Methylmercury (MeHg) may affect fetal growth; however, prior research often lacked assessment of mercury speciation, confounders, and interactions.Our objective was to assess the relationship between MeHg growth as well potential for confounding or interaction this from speciated mercury, fatty acids, selenium, sex.This cross-sectional study includes 271 singletons born in Baltimore, Maryland, 2004-2005. Umbilical cord blood analyzed serum omega-3 highly unsaturated acids (n-3 HUFAs),...
Ruthenium based complexes are promising antitumour candidates due to their lower toxicity and better water-solubility compared the platinum complexes. An epidermal growth factor receptor (EGFR) has been found be overexpressed in a large set of tumour cells. In this work, series organoruthenium containing EGFR-inhibiting 4-anilinoquinazoline pharmacophores were synthesised characterised. These exhibited excellent inhibitory activity against EGFR high affinity interact with DNA via minor...
Abstract Endothelin-1 (ET-1), a vasoconstrictor, has recently been used to induce focal ischemia in rodents and marmoset monkeys. The rhesus monkey, however, numerous advantages the rodent that make it superior irreplaceable animal model for studying stroke brain. In present study, after mapping preferred hand representation two healthy male monkeys with intracortical micro-stimulation, ET-1 was microinjected into contralateral motor cortex (M1) its hand. had trained three manual dexterity...
Human indoleamine 2,3-dioxygenase (hIDO) is an intracellular heme-containing enzyme, which catalyzes the initial and rate-determining step of l-tryptophan (l-Trp) metabolism via kynurenine pathway in nonhepatic tissues. Steady-state kinetic data showed that hIDO exhibits substrate inhibition behavior, implying existence a second binding site although so far there no direct evidence supporting it. The also revealed K(m) l-Trp (15 microM) approximately 27-fold lower than K(d) (0.4 mM) for...