A5100389826- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Parallel Computing and Optimization Techniques
- Colorectal Cancer Treatments and Studies
- Pluripotent Stem Cells Research
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Colorectal Cancer Surgical Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Colorectal and Anal Carcinomas
- Nerve injury and regeneration
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Photoreceptor and optogenetics research
- Embedded Systems Design Techniques
- Ion channel regulation and function
- Gastric Cancer Management and Outcomes
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Pregnancy and preeclampsia studies
- Advanced Data Storage Technologies
Beijing Jiaotong University
2023-2025
Sun Yat-sen University Cancer Center
2016-2025
Hunan Provincial Maternal and Child Health Hospital
2025
Sun Yat-sen University
2016-2025
Anhui Medical University
2020-2025
Chongqing Medical University
2024-2025
Second Affiliated Hospital of Chongqing Medical University
2024-2025
Beijing Anzhen Hospital
2025
Capital Medical University
2025
Dalian Medical University
2024-2025
Migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) that does not correlate with formula molecular weights, termed "gel shifting," appears to be common for membrane proteins but has yet conclusively explained. In the present work, we investigate anomalous mobility of helical using a library wild-type and mutant helix-loop-helix ("hairpin") sequences derived from transmembrane segments 3 4 human cystic fibrosis conductance regulator (CFTR), including...
Atrial fibrillation (AF) is frequently associated with enhanced inflammatory response. The NLRP3 (NACHT, LRR, and PYD domain containing protein 3) inflammasome mediates caspase-1 activation interleukin-1β release in immune cells but not known to play a role cardiomyocytes (CMs). Here, we assessed the of CM AF.
Adult mammalian brains have largely lost neuroregeneration capability except for a few niches. Previous studies converted glial cells into neurons, but the total number of neurons generated is limited and therapeutic potential unclear. Here, we demonstrate that NeuroD1-mediated in situ astrocyte-to-neuron conversion can regenerate large functional new after ischemic injury. Specifically, using NeuroD1 adeno-associated virus (AAV)-based gene therapy, were able to one third caused by injury...
Significance Rett syndrome is a devastating neurodevelopmental disorder that currently has no cure. In this work, we demonstrate human neurons derived from patients with show significant deficit in neuron-specific K + -Cl − cotransporter2 (KCC2) expression, resulting delayed GABA functional switch. Restoring KCC2 level rescues deficits neurons. We further methyl CpG binding protein 2 regulates expression through inhibiting RE1-silencing transcriptional factor. Our data suggest potential...
Abstract Huntington’s disease (HD) is caused by Huntingtin (Htt) gene mutation resulting in the loss of striatal GABAergic neurons and motor functional deficits. We report here an vivo cell conversion technology to reprogram astrocytes into both R6/2 YAC128 HD mouse models through AAV-mediated ectopic expression NeuroD1 Dlx2 transcription factors. found that astrocyte-to-neuron (AtN) rate reached 80% striatum >50% converted were DARPP32 + medium spiny neurons. The astrocyte-converted...
1. Gramicidin-perforated patch clamp recording was employed to study GABA-mediated responses in rat hypothalamic neurones (n = 102) with an intracellular Cl- concentration unaltered by the pipette solution. 2. In young cultures after 1-7 days vitro (DIV), GABA induced depolarizing membrane potentials (+16.5 +/- 1.3 mV) that often surpassed threshold for firing of action (-42 1 and resulted increase neuronal activity. The were dose dependent. necessary evoke half-maximal depolarization (EC50)...
GABA is the dominant inhibitory neurotransmitter in CNS. By opening Cl − channels, generally hyperpolarizes membrane potential, decreases neuronal activity, and reduces intracellular Ca 2+ of mature neurons. In present experiment, we show that after trauma, GABA, both synaptically released exogenously applied, exerted a novel opposite effect, depolarizing neurons increasing . Different types trauma were effective included neurite transection, replating, osmotic imbalance, excess heat. The...
Golgi-specific DHHC (Asp-His-His-Cys) zinc finger protein (GODZ) is a family palmitoyl acyltransferase that implicated in palmitoylation and regulated trafficking of diverse substrates function either at inhibitory or excitatory synapses. Of particular interest the γ2 subunit GABA A receptors, which required for targeting these receptors to Here, we report GODZ and, lesser extent, its close paralog sertoli cell gene with domain-β (SERZ-β) are main members enzymes able palmitoylate...
MiR-637 (microRNA-637) is a primate-specific miRNA belonging to the small noncoding RNA family, which represses gene regulation at post-transcriptional expression level. Although it was discovered approximately 5 years ago, its biomedical significance and regulatory mechanism remain obscure. Our preliminary data showed that miR-637 significantly suppressed in four HCC cell lines and, also, most of hepatocellular carcinoma (HCC) specimens, thereby suggesting would be tumor suppressor HCC....
Schizophrenia is a severe chronic mental disorder with high genetic component in its etiology.Several lines of study have suggested that synaptic dysfunction may underlie the pathogenesis schizophrenia.Neuroligin proteins function as cell-adhesion molecules at post-synaptic membrane and play critical roles synaptogenesis maturation.In this study, we systemically sequenced all exons promoter region neuroligin-2 (NLGN2) gene sample 584 schizophrenia patients 549 control subjects from Taiwan.In...
ABSTRACT Colistin-induced nephrotoxicity is a dose-limiting adverse effect when colistin used against Gram-negative pathogens. This study examined the nephroprotective of melatonin in rats. Rats ( n = 7 per group) were treated intravenously twice daily with saline, (at increasing doses from 0.5 to 4.0 mg/kg), (5 or both and for days. The severity renal alteration was biochemically histologically. coadministration on pharmacokinetics investigated. Significantly lower urinary N -acetyl-β- d...
Abstract Surgical stress and inflammatory response induce the release of catecholamines PGs, which may be key factors in facilitating cancer recurrence through immunosuppression. Animal studies have suggested efficacy perioperative blockades PGs reducing In this study, to our knowledge, we present first report effects propranolol and/or parecoxib on peripheral regulatory T cells (Tregs) breast patients. Patients were randomly assigned control, propranolol, parecoxib, plus groups. We...